##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AccIII AclI AgeI AhdI Aor51HI
AscI AspEI BbeI BciVI BglII
BlpI Bpu1102I BsaBI BsaWI BseAI
BsiWI BsmBI BspDI BspEI BsrBI
BsrDI BsrFI BssHII Bst1107I BstEII
BstPI BstZ17I CelII Cfr10I ClaI
CpoI DrdI Eam1105I Eco47III EcoO65I
EcoRV FseI HgaI HindIII KasI
MamI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PinAI
PmeI PshAI Psp1406I RsrII SalI
SbfI SfoI SgrAI SpeI Sse8387I
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 689 GACGTC GACGT^C
Acc65I 572 GGTACC G^GTACC
AccI 5915 GTAGAC GT^[AC][GT]AC
AcyI 689 GACGTC G[GA]^CG[CT]C
AflII 4132 CTTAAG C^TTAAG
Alw44I 3542 GTGCAC G^TGCAC
ApaLI 3542 GTGCAC G^TGCAC
Asp718I 572 GGTACC G^GTACC
AvaI 241 CCCGGG C^[CT]CG[GA]G
BbrPI 301 CACGTG CAC^GTG
BcgI 4729 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4132 CTTAAG C^TTAAG
BglI 253 GCCGATCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 689 GACGTC G[GA]^CG[CT]C
BsoBI 241 CCCGGG C^[CT]CG[GA]G
Bsp1407I 3806 TGTACA T^GTACA
BsrGI 3806 TGTACA T^GTACA
EagI 251 CGGCCG C^GGCCG
EclXI 251 CGGCCG C^GGCCG
Eco52I 251 CGGCCG C^GGCCG
Hin1I 689 GACGTC G[GA]^CG[CT]C
HpaI 3139 GTTAAC GTT^AAC
KpnI 572 GGTACC GGTAC^C
KspI 106 CCGCGG CCGC^GG
NcoI 3326 CCATGG C^CATGG
PmaCI 301 CACGTG CAC^GTG
PmlI 301 CACGTG CAC^GTG
PvuI 255 CGATCG CGAT^CG
SacII 106 CCGCGG CCGC^GG
SapI 1698 GCTCTTC GCTCTTC(1/4)
SexAI 1376 ACCAGGT A^CC[AT]GGT
SfiI 252 GGCCGATCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 241 CCCGGG CCC^GGG
SnaBI 971 TACGTA TAC^GTA
SspBI 3806 TGTACA T^GTACA
XcmI 3097 CCAAGAGGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 241 CCCGGG C^CCGGG
XmaI 241 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 107 CGCG CG^CG
210 CGCG
AflIII 498 ACATGT A^C[GA][CT]GT
5175 ACATGT
AlwNI 598 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
5720 CAGCTTCTG
ApaI 5425 GGGCCC GGGCC^C
5967 GGGCCC
AspI 3418 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3588 GACAGGGTC
AviII 116 TGCGCA TGC^GCA
269 TGCGCA
BclI 953 TGATCA T^GATCA
7048 TGATCA
BsiEI 251 CGGCCG CG[GA][CT]^CG
255 CGATCG
BspLU11I 498 ACATGT A^CATGT
5175 ACATGT
BssSI 1457 CACGAG CACGAG(-5/-1)
2900 CTCGTG
BstBI 1055 TTCGAA TT^CGAA
1447 TTCGAA
BstUI 107 CGCG CG^CG
210 CGCG
DraIII 5399 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
6999 CACTTAGTG
DsaI 106 CCGCGG C^C[GA][CT]GG
3326 CCATGG
EcoRI 763 GAATTC G^AATTC
3483 GAATTC
EcoT22I 1513 ATGCAT ATGCA^T
4212 ATGCAT
FbaI 953 TGATCA T^GATCA
7048 TGATCA
FspI 116 TGCGCA TGC^GCA
269 TGCGCA
HaeII 229 AGCGCC [GA]GCGC^[CT]
5547 AGCGCC
MvnI 107 CGCG CG^CG
210 CGCG
NsiI 1513 ATGCAT ATGCA^T
4212 ATGCAT
NspV 1055 TTCGAA TT^CGAA
1447 TTCGAA
PflFI 3418 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3588 GACAGGGTC
PpuMI 2168 AGGTCCC [GA]G^G[AT]CC[CT]
5158 AGGACCC
ScaI 868 AGTACT AGT^ACT
3724 AGTACT
SfuI 1055 TTCGAA TT^CGAA
1447 TTCGAA
SphI 1344 GCATGC GCATG^C
6053 GCATGC
Tth111I 3418 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3588 GACAGGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI AseI AsnI Asp700I
AspHI AvaII AvrII BalI BamHI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BlnI BmyI BpmI
BpuAI BsaAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspHI BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PshBI PstI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XhoII XmnI