##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI AscI Asp718I AspEI
AviII BbeI BbrPI BglI BsaAI
BsaHI BseAI BsiEI BsiWI BspDI
BspEI BsrBI BsrFI BssHII Bst1107I
BstZ17I Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoRV FseI FspI Hin1I HpaI
KasI KpnI KspI MluI MroI
NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SnaBI
Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AflII 3200 CTTAAG C^TTAAG
AflIII 1982 ACATGT A^C[GA][CT]GT
Alw44I 1446 GTGCAC G^TGCAC
AocI 703 CCTAAGG CC^T[ACGT]AGG
Aor51HI 2240 AGCGCT AGC^GCT
ApaLI 1446 GTGCAC G^TGCAC
AseI 5282 ATTAAT AT^TAAT
AsnI 5282 ATTAAT AT^TAAT
AvrII 2816 CCTAGG C^CTAGG
BamHI 149 GGATCC G^GATCC
BbvCI 4839 GCTGAGG CCTCAGC(-5/-2)
BcgI 2035 CGAGCTCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3200 CTTAAG C^TTAAG
BglII 110 AGATCT A^GATCT
BlnI 2816 CCTAGG C^CTAGG
BlpI 126 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 126 GCTGAGC GC^T[ACGT]AGC
BsaBI 105 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1179 ACCGGA [AT]^CCGG[AT]
Bsp1407I 5810 TGTACA T^GTACA
BspLU11I 1982 ACATGT A^CATGT
BsrGI 5810 TGTACA T^GTACA
BssSI 2086 CTCGTG CACGAG(-5/-1)
BstBI 910 TTCGAA TT^CGAA
BstXI 4812 CCATGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 703 CCTAAGG CC^T[ACGT]AGG
CelII 126 GCTGAGC GC^T[ACGT]AGC
Eco47III 2240 AGCGCT AGC^GCT
Eco81I 703 CCTAAGG CC^T[ACGT]AGG
FauI 3479 CCCGC CCCGC(4/6)
HaeII 2240 AGCGCT [GA]GCGC^[CT]
HgaI 1262 GCGTC GACGC(5/10)
MamI 105 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspV 910 TTCGAA TT^CGAA
PaeR7I 5542 CTCGAG C^TCGAG
PshBI 5282 ATTAAT AT^TAAT
SfuI 910 TTCGAA TT^CGAA
SmaI 179 CCCGGG CCC^GGG
SpeI 3278 ACTAGT A^CTAGT
SphI 5786 GCATGC GCATG^C
SspBI 5810 TGTACA T^GTACA
XbaI 2142 TCTAGA T^CTAGA
XcmI 512 CCATCGCGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 5542 CTCGAG C^TCGAG
XmaCI 179 CCCGGG C^CCGGG
XmaI 179 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 516 CGCG CG^CG
1003 CGCG
Asp700I 5953 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6010 GAAATATTTC
AvaI 179 CCCGGG C^[CT]CG[GA]G
5542 CTCGAG
BanI 84 GGCACC G^G[CT][GA]CC
4871 GGCACC
BciVI 4514 GGATAC GTATCC(6/5)
2465 GTATCC
BclI 1292 TGATCA T^GATCA
1848 TGATCA
BsoBI 179 CCCGGG C^[CT]CG[GA]G
5542 CTCGAG
BsrDI 5296 CATTGC GCAATG(2/0)
944 GCAATG
BstEII 3368 GGTCACC G^GT[ACGT]ACC
5100 GGTTACC
BstPI 3368 GGTCACC G^GT[ACGT]ACC
5100 GGTTACC
BstUI 516 CGCG CG^CG
1003 CGCG
CfoI 1002 GCGC GCG^C
2241 GCGC
EcoNI 286 CCTGACTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1641 CCTCCCAGAGG
EcoO65I 3368 GGTCACC G^GT[ACGT]ACC
5100 GGTTACC
EcoT22I 4711 ATGCAT ATGCA^T
5659 ATGCAT
FbaI 1292 TGATCA T^GATCA
1848 TGATCA
HhaI 1002 GCGC GCG^C
2241 GCGC
HinP1I 1002 GCGC G^CGC
2241 GCGC
HincII 1420 GTTGAC GT[CT]^[GA]AC
1529 GTCAAC
HindII 1420 GTTGAC GT[CT]^[GA]AC
1529 GTCAAC
MaeII 3498 ACGT A^CGT
4029 ACGT
MfeI 782 CAATTG C^AATTG
4492 CAATTG
MunI 782 CAATTG C^AATTG
4492 CAATTG
MvnI 516 CGCG CG^CG
1003 CGCG
NsiI 4711 ATGCAT ATGCA^T
5659 ATGCAT
SacI 2036 GAGCTC GAGCT^C
2343 GAGCTC
SapI 12 GAAGAGC GCTCTTC(1/4)
4446 GAAGAGC
SspI 4562 AATATT AAT^ATT
6012 AATATT
StuI 172 AGGCCT AGG^CCT
3584 AGGCCT
XmnI 5953 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6010 GAAATATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AspI AvaII BalI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI Bsp1286I BspHI BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XhoII