################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## Acc65I AccIII AclI AflII AscI AseI AsnI Asp700I Asp718I AvrII BbvCI BfrI BlnI BseAI BsiWI BsmBI BspDI BspEI Bst1107I BstBI BstZ17I ClaI CpoI DraI EcoRI EcoT22I HindIII HpaI KpnI KspI MfeI MluI MroI MseI MunI NdeI NheI NruI NsiI NspV PacI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SapI SexAI SfiI SfuI SnaBI SpeI SspI SwaI TfiI Tru9I XbaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccI 3558 GTCTAC GT^[AC][GT]AC AcsI 137 GAATTT [GA]^AATT[CT] AgeI 1261 ACCGGT A^CCGGT AhdI 4518 GACCGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 137 GAATTT [GA]^AATT[CT] AspEI 4518 GACCGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 949 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 1610 GGATCC G^GATCC BclI 2671 TGATCA T^GATCA BglII 4813 AGATCT A^GATCT BsaBI 4634 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmI 1398 GAATGC GAATGC(1/-1) Bsp1407I 2848 TGTACA T^GTACA BsrDI 4888 GCAATG GCAATG(2/0) BsrGI 2848 TGTACA T^GTACA BssHII 997 GCGCGC G^CGCGC BstEII 257 GGTCACC G^GT[ACGT]ACC BstPI 257 GGTCACC G^GT[ACGT]ACC Eam1105I 4518 GACCGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 257 GGTCACC G^GT[ACGT]ACC EcoRV 288 GATATC GAT^ATC FbaI 2671 TGATCA T^GATCA FseI 1214 GGCCGGCC GGCCGG^CC MamI 4634 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NotI 5206 GCGGCCGC GC^GGCCGC PaeR7I 2097 CTCGAG C^TCGAG PflFI 949 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 1261 ACCGGT A^CCGGT PshAI 1943 GACGGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SbfI 1182 CCTGCAGG CCTGCA^GG ScaI 64 AGTACT AGT^ACT SgrAI 611 CACCGGCG C[GA]^CCGG[CT]G SmlI 2097 CTCGAG C^T[CT][GA]AG SphI 1302 GCATGC GCATG^C Sse8387I 1182 CCTGCAGG CCTGCA^GG SspBI 2848 TGTACA T^GTACA Tth111I 949 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 2097 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AviII 3621 TGCGCA TGC^GCA 4834 TGCGCA BcgI 747 CGATGCTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3734 CGAAGTAGGTGC BciVI 2879 GGATAC GTATCC(6/5) 3831 GGATAC BfaI 2452 CTAG C^TAG 2742 CTAG BlpI 2648 GCTCAGC GC^T[ACGT]AGC 4376 GCTGAGC Bpu1102I 2648 GCTCAGC GC^T[ACGT]AGC 4376 GCTGAGC BsaWI 1261 ACCGGT [AT]^CCGG[AT] 3828 ACCGGA BspHI 4759 TCATGA T^CATGA 5107 TCATGA BspLU11I 1119 ACATGT A^CATGT 1536 ACATGT CelII 2648 GCTCAGC GC^T[ACGT]AGC 4376 GCTGAGC DraIII 1759 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG 2855 CACCCTGTG DrdI 249 GACCCCACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 830 GACGCCCTCGTC FspI 3621 TGCGCA TGC^GCA 4834 TGCGCA HincII 618 GTCAAC GT[CT]^[GA]AC 2214 GTCAAC HindII 618 GTCAAC GT[CT]^[GA]AC 2214 GTCAAC MaeI 2452 CTAG C^TAG 2742 CTAG RcaI 4759 TCATGA T^CATGA 5107 TCATGA SmaI 2875 CCCGGG CCC^GGG 3016 CCCGGG StuI 1828 AGGCCT AGG^CCT 2497 AGGCCT Tsp509I 138 AATT ^AATT 888 AATT XmaCI 2875 CCCGGG C^CCGGG 3016 CCCGGG XmaI 2875 CCCGGG C^CCGGG 3016 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 138 enzymes) # ######################################### AatII AccII AciI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AspHI AvaI AvaII BalI BanI BanII BbeI BbrPI BbsI BbvI BcnI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII