##################################
# NO CUT ENZYMES (   60 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AscI        
AseI        AsnI        Asp700I     Asp718I     AvrII       
BbvCI       BfrI        BlnI        BseAI       BsiWI       
BsmBI       BspDI       BspEI       Bst1107I    BstBI       
BstZ17I     ClaI        CpoI        DraI        EcoRI       
EcoT22I     HindIII     HpaI        KpnI        KspI        
MfeI        MluI        MroI        MseI        MunI        
NdeI        NheI        NruI        NsiI        NspV        
PacI        PmeI        PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SapI        SexAI       
SfiI        SfuI        SnaBI       SpeI        SspI        
SwaI        TfiI        Tru9I       XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccI         3558  GTCTAC               GT^[AC][GT]AC
AcsI          137  GAATTT               [GA]^AATT[CT]
AgeI         1261  ACCGGT               A^CCGGT
AhdI         4518  GACCGGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI          137  GAATTT               [GA]^AATT[CT]
AspEI        4518  GACCGGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          949  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        1610  GGATCC               G^GATCC
BclI         2671  TGATCA               T^GATCA
BglII        4813  AGATCT               A^GATCT
BsaBI        4634  GATGAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI         1398  GAATGC               GAATGC(1/-1)
Bsp1407I     2848  TGTACA               T^GTACA
BsrDI        4888  GCAATG               GCAATG(2/0)
BsrGI        2848  TGTACA               T^GTACA
BssHII        997  GCGCGC               G^CGCGC
BstEII        257  GGTCACC              G^GT[ACGT]ACC
BstPI         257  GGTCACC              G^GT[ACGT]ACC
Eam1105I     4518  GACCGGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I       257  GGTCACC              G^GT[ACGT]ACC
EcoRV         288  GATATC               GAT^ATC
FbaI         2671  TGATCA               T^GATCA
FseI         1214  GGCCGGCC             GGCCGG^CC
MamI         4634  GATGAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NotI         5206  GCGGCCGC             GC^GGCCGC
PaeR7I       2097  CTCGAG               C^TCGAG
PflFI         949  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        1261  ACCGGT               A^CCGGT
PshAI        1943  GACGGGCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI         1182  CCTGCAGG             CCTGCA^GG
ScaI           64  AGTACT               AGT^ACT
SgrAI         611  CACCGGCG             C[GA]^CCGG[CT]G
SmlI         2097  CTCGAG               C^T[CT][GA]AG
SphI         1302  GCATGC               GCATG^C
Sse8387I     1182  CCTGCAGG             CCTGCA^GG
SspBI        2848  TGTACA               T^GTACA
Tth111I       949  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         2097  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AviII        3621  TGCGCA               TGC^GCA
             4834  TGCGCA              
BcgI          747  CGATGCTACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3734  CGAAGTAGGTGC        
BciVI        2879  GGATAC               GTATCC(6/5)
             3831  GGATAC              
BfaI         2452  CTAG                 C^TAG
             2742  CTAG                
BlpI         2648  GCTCAGC              GC^T[ACGT]AGC
             4376  GCTGAGC             
Bpu1102I     2648  GCTCAGC              GC^T[ACGT]AGC
             4376  GCTGAGC             
BsaWI        1261  ACCGGT               [AT]^CCGG[AT]
             3828  ACCGGA              
BspHI        4759  TCATGA               T^CATGA
             5107  TCATGA              
BspLU11I     1119  ACATGT               A^CATGT
             1536  ACATGT              
CelII        2648  GCTCAGC              GC^T[ACGT]AGC
             4376  GCTGAGC             
DraIII       1759  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2855  CACCCTGTG           
DrdI          249  GACCCCACGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              830  GACGCCCTCGTC        
FspI         3621  TGCGCA               TGC^GCA
             4834  TGCGCA              
HincII        618  GTCAAC               GT[CT]^[GA]AC
             2214  GTCAAC              
HindII        618  GTCAAC               GT[CT]^[GA]AC
             2214  GTCAAC              
MaeI         2452  CTAG                 C^TAG
             2742  CTAG                
RcaI         4759  TCATGA               T^CATGA
             5107  TCATGA              
SmaI         2875  CCCGGG               CCC^GGG
             3016  CCCGGG              
StuI         1828  AGGCCT               AGG^CCT
             2497  AGGCCT              
Tsp509I       138  AATT                 ^AATT
              888  AATT                
XmaCI        2875  CCCGGG               C^CCGGG
             3016  CCCGGG              
XmaI         2875  CCCGGG               C^CCGGG
             3016  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  138 enzymes) #
#########################################

AatII       AccII       AciI        AcyI        AfaI        
AflIII      AluI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbeI        BbrPI       BbsI        BbvI        BcnI        
BglI        BmyI        BpmI        BpuAI       BsaAI       
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrFI       BsrI        BssKI       BssSI       BstAPI      
BstF5I      BstNI       BstUI       BstXI       BstYI       
Bsu36I      Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EagI        EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        KasI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeII       
NgoMIV      NlaIII      NlaIV       NspI        PflMI       
PleI        PmaCI       PmlI        PpuMI       PstI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfoI        StyI        
TaqI        TseI        Tsp45I      TspRI       TthHB8I     
Van91I      XcmI        XhoII