##################################
# NO CUT ENZYMES (   63 enzymes) #
##################################

AccI        AccIII      AclI        AcsI        AflII       
AgeI        ApoI        AscI        AseI        AsnI        
BbsI        BciVI       BclI        BfrI        BglII       
BpuAI       BsaBI       BsaWI       BseAI       BsiWI       
BsmI        BspDI       BspEI       BspLU11I    Bst1107I    
BstBI       BstZ17I     ClaI        DraI        EcoRI       
EcoRV       EcoT22I     FbaI        FseI        HindIII     
HpaI        MamI        MfeI        MluI        MroI        
MunI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        SalI        
SfiI        SfuI        SnaBI       SpeI        SphI        
SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   27 enzymes) #
###################################

Acc65I       4065  GGTACC               G^GTACC
AhdI         4225  GACTGAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       3356  GTGCAC               G^TGCAC
ApaLI        3356  GTGCAC               G^TGCAC
Asp718I      4065  GGTACC               G^GTACC
AspEI        4225  GACTGAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         609  TGCGCA               TGC^GCA
AvrII        1420  CCTAGG               C^CTAGG
BamHI        1563  GGATCC               G^GATCC
BlnI         1420  CCTAGG               C^CTAGG
CpoI         4791  CGGACCG              CG^G[AT]CCG
Eam1105I     4225  GACTGAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI          609  TGCGCA               TGC^GCA
KpnI         4065  GGTACC               GGTAC^C
NdeI         2873  CATATG               CA^TATG
NheI          108  GCTAGC               G^CTAGC
PflMI         170  CCAGCTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsrII        4791  CGGACCG              CG^G[AT]CCG
SbfI         4033  CCTGCAGG             CCTGCA^GG
ScaI         4475  AGTACT               AGT^ACT
SexAI        5232  ACCAGGT              A^CC[AT]GGT
SgrAI         753  CGCCGGTG             C[GA]^CCGG[CT]G
Sse8387I     4033  CCTGCAGG             CCTGCA^GG
SspI         4492  AATATT               AAT^ATT
TfiI         2200  GATTC                G^A[AT]TC
Tsp509I      1731  AATT                 ^AATT
Van91I        170  CCAGCTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AatII        1490  GACGTC               GACGT^C
             3537  GACGTC              
AflIII       3249  ACGTGT               A^C[GA][CT]GT
             3768  ACACGT              
Asp700I      2473  GAACCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3457  GAATATGTTC          
AspI         1370  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1703  GACGTGGTC           
BbvCI        5031  CCTCAGC              CCTCAGC(-5/-2)
             2366  GCTGAGG             
Bsp1407I     2562  TGTACA               T^GTACA
             3435  TGTACA              
BspHI        2649  TCATGA               T^CATGA
             2910  TCATGA              
BsrGI        2562  TGTACA               T^GTACA
             3435  TGTACA              
BssSI        3796  CACGAG               CACGAG(-5/-1)
             2972  CTCGTG              
BstYI        1563  GGATCC               [GA]^GATC[CT]
             2054  GGATCT              
MflI         1563  GGATCC               [GA]^GATC[CT]
             2054  GGATCT              
PflFI        1370  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1703  GACGTGGTC           
RcaI         2649  TCATGA               T^CATGA
             2910  TCATGA              
SacI          593  GAGCTC               GAGCT^C
             3032  GAGCTC              
SapI          208  GCTCTTC              GCTCTTC(1/4)
             1936  GCTCTTC             
SspBI        2562  TGTACA               T^GTACA
             3435  TGTACA              
StuI         1848  AGGCCT               AGG^CCT
             4701  AGGCCT              
Tth111I      1370  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1703  GACGTGGTC           
XhoII        1563  GGATCC               [GA]^GATC[CT]
             2054  GGATCT              
XmnI         2473  GAACCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3457  GAATATGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  148 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AocI        Aor51HI     ApaI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbeI        BbrPI       BbvI        BcgI        
BcnI        BfaI        BglI        BlpI        BmyI        
BpmI        Bpu1102I    BsaAI       BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrDI       
BsrFI       BsrI        BssHII      BssKI       BstAPI      
BstEII      BstF5I      BstNI       BstPI       BstUI       
BstXI       Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        KasI        Ksp632I     KspI        MaeI        
MaeII       MaeIII      MboI        MboII       MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NdeII       NgoMIV      
NlaIII      NlaIV       PleI        PmaCI       PmlI        
PpuMI       PstI        PvuII       RsaI        SacII       
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SfoI        SmaI        SmlI        StyI        TaqI        
Tru9I       TseI        Tsp45I      TspRI       TthHB8I     
XcmI        XmaCI       XmaI