##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AccI AccIII AclI AcsI AflII
AgeI ApoI AscI AseI AsnI
BbsI BciVI BclI BfrI BglII
BpuAI BsaBI BsaWI BseAI BsiWI
BsmI BspDI BspEI BspLU11I Bst1107I
BstBI BstZ17I ClaI DraI EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HpaI MamI MfeI MluI MroI
MunI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI SalI
SfiI SfuI SnaBI SpeI SphI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 4065 GGTACC G^GTACC
AhdI 4225 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3356 GTGCAC G^TGCAC
ApaLI 3356 GTGCAC G^TGCAC
Asp718I 4065 GGTACC G^GTACC
AspEI 4225 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 609 TGCGCA TGC^GCA
AvrII 1420 CCTAGG C^CTAGG
BamHI 1563 GGATCC G^GATCC
BlnI 1420 CCTAGG C^CTAGG
CpoI 4791 CGGACCG CG^G[AT]CCG
Eam1105I 4225 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 609 TGCGCA TGC^GCA
KpnI 4065 GGTACC GGTAC^C
NdeI 2873 CATATG CA^TATG
NheI 108 GCTAGC G^CTAGC
PflMI 170 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsrII 4791 CGGACCG CG^G[AT]CCG
SbfI 4033 CCTGCAGG CCTGCA^GG
ScaI 4475 AGTACT AGT^ACT
SexAI 5232 ACCAGGT A^CC[AT]GGT
SgrAI 753 CGCCGGTG C[GA]^CCGG[CT]G
Sse8387I 4033 CCTGCAGG CCTGCA^GG
SspI 4492 AATATT AAT^ATT
TfiI 2200 GATTC G^A[AT]TC
Tsp509I 1731 AATT ^AATT
Van91I 170 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AatII 1490 GACGTC GACGT^C
3537 GACGTC
AflIII 3249 ACGTGT A^C[GA][CT]GT
3768 ACACGT
Asp700I 2473 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3457 GAATATGTTC
AspI 1370 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1703 GACGTGGTC
BbvCI 5031 CCTCAGC CCTCAGC(-5/-2)
2366 GCTGAGG
Bsp1407I 2562 TGTACA T^GTACA
3435 TGTACA
BspHI 2649 TCATGA T^CATGA
2910 TCATGA
BsrGI 2562 TGTACA T^GTACA
3435 TGTACA
BssSI 3796 CACGAG CACGAG(-5/-1)
2972 CTCGTG
BstYI 1563 GGATCC [GA]^GATC[CT]
2054 GGATCT
MflI 1563 GGATCC [GA]^GATC[CT]
2054 GGATCT
PflFI 1370 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1703 GACGTGGTC
RcaI 2649 TCATGA T^CATGA
2910 TCATGA
SacI 593 GAGCTC GAGCT^C
3032 GAGCTC
SapI 208 GCTCTTC GCTCTTC(1/4)
1936 GCTCTTC
SspBI 2562 TGTACA T^GTACA
3435 TGTACA
StuI 1848 AGGCCT AGG^CCT
4701 AGGCCT
Tth111I 1370 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1703 GACGTGGTC
XhoII 1563 GGATCC [GA]^GATC[CT]
2054 GGATCT
XmnI 2473 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3457 GAATATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI Aor51HI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbrPI BbvI BcgI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssHII BssKI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DrdI DsaI EaeI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI StyI TaqI
Tru9I TseI Tsp45I TspRI TthHB8I
XcmI XmaCI XmaI