##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
Acc65I AccIII AclI AgeI AscI
AseI AsnI Asp718I AviII AvrII
BlnI BseAI BsiWI BspDI BspEI
BsrBI Bst1107I BstBI BstZ17I ClaI
CpoI DraI EagI EclXI Eco52I
EcoRV EcoT22I FseI FspI KpnI
MfeI MluI MroI MunI NaeI
NgoMIV NheI NotI NsiI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI RsrII SalI SfiI SfuI
SgrAI SnaBI SpeI SspI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AflII 5751 CTTAAG C^TTAAG
Alw44I 4096 GTGCAC G^TGCAC
Aor51HI 4829 AGCGCT AGC^GCT
ApaLI 4096 GTGCAC G^TGCAC
Asp700I 1707 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 5824 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1335 GGCGCC GGCGC^C
BcgI 172 GCAGCTGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 642 TGATCA T^GATCA
BfrI 5751 CTTAAG C^TTAAG
BsaBI 4465 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 2928 CGACCG CG[GA][CT]^CG
BsmBI 1823 GAGACG CGTCTC(1/5)
BspLU11I 944 ACATGT A^CATGT
BsrDI 3022 CATTGC GCAATG(2/0)
DrdI 233 GACTCTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4829 AGCGCT AGC^GCT
FbaI 642 TGATCA T^GATCA
HpaI 1882 GTTAAC GTT^AAC
KasI 1335 GGCGCC G^GCGCC
KspI 3990 CCGCGG CCGC^GG
MamI 4465 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1335 GGCGCC GG^CGCC
NdeI 57 CATATG CA^TATG
NruI 4278 TCGCGA TCG^CGA
PaeR7I 333 CTCGAG C^TCGAG
PflFI 5824 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3178 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 3990 CCGCGG CCGC^GG
SbfI 1927 CCTGCAGG CCTGCA^GG
ScaI 5586 AGTACT AGT^ACT
SfoI 1335 GGCGCC GGC^GCC
Sse8387I 1927 CCTGCAGG CCTGCA^GG
Tth111I 5824 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 333 CTCGAG C^TCGAG
XmnI 1707 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 4175 GAATTC [GA]^AATT[CT]
5628 GAATTC
ApoI 4175 GAATTC [GA]^AATT[CT]
5628 GAATTC
BbrPI 3124 CACGTG CAC^GTG
4691 CACGTG
BbsI 3557 GAAGAC GAAGAC(2/6)
3982 GAAGAC
BciVI 5092 GGATAC GTATCC(6/5)
2173 GTATCC
BglII 2146 AGATCT A^GATCT
4251 AGATCT
BpuAI 3557 GAAGAC GAAGAC(2/6)
3982 GAAGAC
BsmI 962 GCATTC GAATGC(1/-1)
2905 GCATTC
Bsp1407I 39 TGTACA T^GTACA
1551 TGTACA
BspHI 2760 TCATGA T^CATGA
3978 TCATGA
BsrGI 39 TGTACA T^GTACA
1551 TGTACA
BssHII 1962 GCGCGC G^CGCGC
1964 GCGCGC
BssSI 5300 CTCGTG CACGAG(-5/-1)
5426 CTCGTG
EcoRI 4175 GAATTC G^AATTC
5628 GAATTC
HindIII 1386 AAGCTT A^AGCTT
2955 AAGCTT
PmaCI 3124 CACGTG CAC^GTG
4691 CACGTG
PmlI 3124 CACGTG CAC^GTG
4691 CACGTG
RcaI 2760 TCATGA T^CATGA
3978 TCATGA
SapI 1409 GAAGAGC GCTCTTC(1/4)
4832 GCTCTTC
SphI 616 GCATGC GCATG^C
1279 GCATGC
SspBI 39 TGTACA T^GTACA
1551 TGTACA
StuI 329 AGGCCT AGG^CCT
4858 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
AatII AccI AccII AciI AcyI
AfaI AflIII AhdI AluI AlwI
AlwNI AocI ApaI AspEI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI Eam1105I EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI