##################################
# NO CUT ENZYMES (   80 enzymes) #
##################################

AccII       AclI        AflII       AgeI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        AvaI        
AviII       AvrII       BbeI        BbrPI       BcgI        
BfrI        BglI        BlnI        BsaBI       BsiEI       
BsiWI       BsmBI       BsoBI       Bsp1407I    BspDI       
BsrBI       BsrGI       BssHII      BssSI       BstEII      
BstPI       BstUI       ClaI        CpoI        DraIII      
EagI        EclXI       Eco47III    Eco52I      EcoO65I     
EcoT22I     FseI        FspI        HaeII       HgaI        
KasI        KspI        MamI        MluI        MvnI        
NaeI        NarI        NgoMIV      NheI        NotI        
NruI        NsiI        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        Psp1406I    PvuI        
RsrII       SacII       SalI        SbfI        SfiI        
SfoI        SmaI        SnaBI       Sse8387I    SspBI       
SwaI        XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AatII        1989  GACGTC               GACGT^C
Acc65I       1432  GGTACC               G^GTACC
AccI         4275  GTATAC               GT^[AC][GT]AC
AccIII       1213  TCCGGA               T^CCGGA
AcyI         1989  GACGTC               G[GA]^CG[CT]C
AhdI         3217  GACTACCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I      1432  GGTACC               G^GTACC
AspEI        3217  GACTACCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         5632  GACGTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        4169  GGATCC               G^GATCC
BbvCI         540  GCTGAGG              CCTCAGC(-5/-2)
BcnI         1784  CCCGG                CC^[GC]GG
BglII        2255  AGATCT               A^GATCT
BsaAI        3847  CACGTA               [CT]AC^GT[GA]
BsaHI        1989  GACGTC               G[GA]^CG[CT]C
BseAI        1213  TCCGGA               T^CCGGA
BseRI          90  CTCCTC               GAGGAG(10/8)
BsgI         3271  GTGCAG               GTGCAG(16/14)
BspEI        1213  TCCGGA               T^CCGGA
BsrDI        3333  CATTGC               GCAATG(2/0)
BsrFI        3551  GCCGGT               [GA]^CCGG[CT]
Bst1107I     4275  GTATAC               GTA^TAC
BstBI        1892  TTCGAA               TT^CGAA
BstZ17I      4275  GTATAC               GTA^TAC
Cfr10I       3551  GCCGGT               [GA]^CCGG[CT]
DrdI         3606  GACCCGCAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     3217  GACTACCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        1729  CCTGATGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1645  GAATTC               G^AATTC
FauI         3608  CCCGC                CCCGC(4/6)
Hin1I        1989  GACGTC               G[GA]^CG[CT]C
HincII       5166  GTTAAC               GT[CT]^[GA]AC
HindII       5166  GTTAAC               GT[CT]^[GA]AC
HpaI         5166  GTTAAC               GTT^AAC
KpnI         1432  GGTACC               GGTAC^C
MroI         1213  TCCGGA               T^CCGGA
NciI         1784  CCCGG                CC^[GC]GG
NspV         1892  TTCGAA               TT^CGAA
PflFI        5632  GACGTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI         431  GACAGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         1200  GAGCTC               GAGCT^C
SapI         1197  GAAGAGC              GCTCTTC(1/4)
SfuI         1892  TTCGAA               TT^CGAA
SgrAI        3550  CGCCGGTG             C[GA]^CCGG[CT]G
SpeI         1824  ACTAGT               A^CTAGT
SphI         4224  GCATGC               GCATG^C
Tth111I      5632  GACGTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AflIII       4244  ACATGT               A^C[GA][CT]GT
             4853  ACATGT              
BanII        1200  GAGCTC               G[GA]GC[CT]^C
             1739  GGGCTC              
BbsI         1518  GAAGAC               GAAGAC(2/6)
             3316  GTCTTC              
BciVI        3285  GGATAC               GTATCC(6/5)
              256  GTATCC              
BclI         3540  TGATCA               T^GATCA
             4487  TGATCA              
BpuAI        1518  GAAGAC               GAAGAC(2/6)
             3316  GTCTTC              
BsaWI        1213  TCCGGA               [AT]^CCGG[AT]
             1328  ACCGGA              
BspHI        1451  TCATGA               T^CATGA
             1641  TCATGA              
BspLU11I     4244  ACATGT               A^CATGT
             4853  ACATGT              
BstAPI       1354  GCAATACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2082  GCATAAGATGC         
BstXI        1616  CCATTAAAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3245  CCACATCTTTGG        
CfoI         1697  GCGC                 GCG^C
             3994  GCGC                
FbaI         3540  TGATCA               T^GATCA
             4487  TGATCA              
HhaI         1697  GCGC                 GCG^C
             3994  GCGC                
HinP1I       1697  GCGC                 G^CGC
             3994  GCGC                
HindIII      1915  AAGCTT               A^AGCTT
             4730  AAGCTT              
MfeI         4103  CAATTG               C^AATTG
             5192  CAATTG              
MunI         4103  CAATTG               C^AATTG
             5192  CAATTG              
NcoI         3011  CCATGG               C^CATGG
             3239  CCATGG              
NdeI         4865  CATATG               CA^TATG
             4919  CATATG              
RcaI         1451  TCATGA               T^CATGA
             1641  TCATGA              
ScaI         1077  AGTACT               AGT^ACT
             2066  AGTACT              
SexAI         137  ACCTGGT              A^CC[AT]GGT
              387  ACCTGGT             


#########################################
# 3 OR MORE CUT ENZYMES (  108 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       AocI        ApoI        AseI        AsnI        
Asp700I     AspHI       AvaII       BalI        BanI        
BbvI        BfaI        BlpI        BmyI        BpmI        
Bpu1102I    BsaI        BsaJI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Bsu36I      Cac8I       CelII       Cfr13I      
DdeI        DpnII       DraI        DraII       DsaI        
EaeI        EarI        Eco81I      EcoO109I    EcoRII      
EcoRV       EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PshBI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SspI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI        XhoII       XmnI