################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AccII AclI AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI AvaI AviII AvrII BbeI BbrPI BcgI BfrI BglI BlnI BsaBI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BsrBI BsrGI BssHII BssSI BstEII BstPI BstUI ClaI CpoI DraIII EagI EclXI Eco47III Eco52I EcoO65I EcoT22I FseI FspI HaeII HgaI KasI KspI MamI MluI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SmaI SnaBI Sse8387I SspBI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 1989 GACGTC GACGT^C Acc65I 1432 GGTACC G^GTACC AccI 4275 GTATAC GT^[AC][GT]AC AccIII 1213 TCCGGA T^CCGGA AcyI 1989 GACGTC G[GA]^CG[CT]C AhdI 3217 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp718I 1432 GGTACC G^GTACC AspEI 3217 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 5632 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 4169 GGATCC G^GATCC BbvCI 540 GCTGAGG CCTCAGC(-5/-2) BcnI 1784 CCCGG CC^[GC]GG BglII 2255 AGATCT A^GATCT BsaAI 3847 CACGTA [CT]AC^GT[GA] BsaHI 1989 GACGTC G[GA]^CG[CT]C BseAI 1213 TCCGGA T^CCGGA BseRI 90 CTCCTC GAGGAG(10/8) BsgI 3271 GTGCAG GTGCAG(16/14) BspEI 1213 TCCGGA T^CCGGA BsrDI 3333 CATTGC GCAATG(2/0) BsrFI 3551 GCCGGT [GA]^CCGG[CT] Bst1107I 4275 GTATAC GTA^TAC BstBI 1892 TTCGAA TT^CGAA BstZ17I 4275 GTATAC GTA^TAC Cfr10I 3551 GCCGGT [GA]^CCGG[CT] DrdI 3606 GACCCGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 3217 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1729 CCTGATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1645 GAATTC G^AATTC FauI 3608 CCCGC CCCGC(4/6) Hin1I 1989 GACGTC G[GA]^CG[CT]C HincII 5166 GTTAAC GT[CT]^[GA]AC HindII 5166 GTTAAC GT[CT]^[GA]AC HpaI 5166 GTTAAC GTT^AAC KpnI 1432 GGTACC GGTAC^C MroI 1213 TCCGGA T^CCGGA NciI 1784 CCCGG CC^[GC]GG NspV 1892 TTCGAA TT^CGAA PflFI 5632 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 431 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 1200 GAGCTC GAGCT^C SapI 1197 GAAGAGC GCTCTTC(1/4) SfuI 1892 TTCGAA TT^CGAA SgrAI 3550 CGCCGGTG C[GA]^CCGG[CT]G SpeI 1824 ACTAGT A^CTAGT SphI 4224 GCATGC GCATG^C Tth111I 5632 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AflIII 4244 ACATGT A^C[GA][CT]GT 4853 ACATGT BanII 1200 GAGCTC G[GA]GC[CT]^C 1739 GGGCTC BbsI 1518 GAAGAC GAAGAC(2/6) 3316 GTCTTC BciVI 3285 GGATAC GTATCC(6/5) 256 GTATCC BclI 3540 TGATCA T^GATCA 4487 TGATCA BpuAI 1518 GAAGAC GAAGAC(2/6) 3316 GTCTTC BsaWI 1213 TCCGGA [AT]^CCGG[AT] 1328 ACCGGA BspHI 1451 TCATGA T^CATGA 1641 TCATGA BspLU11I 4244 ACATGT A^CATGT 4853 ACATGT BstAPI 1354 GCAATACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2082 GCATAAGATGC BstXI 1616 CCATTAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3245 CCACATCTTTGG CfoI 1697 GCGC GCG^C 3994 GCGC FbaI 3540 TGATCA T^GATCA 4487 TGATCA HhaI 1697 GCGC GCG^C 3994 GCGC HinP1I 1697 GCGC G^CGC 3994 GCGC HindIII 1915 AAGCTT A^AGCTT 4730 AAGCTT MfeI 4103 CAATTG C^AATTG 5192 CAATTG MunI 4103 CAATTG C^AATTG 5192 CAATTG NcoI 3011 CCATGG C^CATGG 3239 CCATGG NdeI 4865 CATATG CA^TATG 4919 CATATG RcaI 1451 TCATGA T^CATGA 1641 TCATGA ScaI 1077 AGTACT AGT^ACT 2066 AGTACT SexAI 137 ACCTGGT A^CC[AT]GGT 387 ACCTGGT ######################################### # 3 OR MORE CUT ENZYMES ( 108 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AseI AsnI Asp700I AspHI AvaII BalI BanI BbvI BfaI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CelII Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoRV EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PshBI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI