################################## # NO CUT ENZYMES ( 56 enzymes) # ################################## AatII AgeI Aor51HI AscI AspI BamHI BbrPI BsiEI BsiWI BspDI BssHII BstBI BstEII BstPI ClaI CpoI DrdI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV FseI HpaI MluI NaeI NcoI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PvuI RsrII SalI ScaI SexAI SfiI SfuI SgrAI SmaI SphI StuI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### Acc65I 3249 GGTACC G^GTACC AccIII 2582 TCCGGA T^CCGGA AclI 4383 AACGTT AA^CGTT AcyI 4000 GGCGCC G[GA]^CG[CT]C AflIII 6271 ACATGT A^C[GA][CT]GT AhdI 5218 GACCTGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 4105 GGGCCC GGGCC^C Asp718I 3249 GGTACC G^GTACC AspEI 5218 GACCTGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 3511 CCCGAG C^[CT]CG[GA]G AviII 3946 TGCGCA TGC^GCA AvrII 1517 CCTAGG C^CTAGG BalI 991 TGGCCA TGG^CCA BbeI 4000 GGCGCC GGCGC^C BcgI 739 CGACCATCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 898 TGATCA T^GATCA BlnI 1517 CCTAGG C^CTAGG BlpI 1057 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1057 GCTCAGC GC^T[ACGT]AGC BsaBI 2004 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 4000 GGCGCC G[GA]^CG[CT]C BseAI 2582 TCCGGA T^CCGGA BsmBI 3610 GAGACG CGTCTC(1/5) BsoBI 3511 CCCGAG C^[CT]CG[GA]G Bsp1407I 5753 TGTACA T^GTACA BspEI 2582 TCCGGA T^CCGGA BspLU11I 6271 ACATGT A^CATGT BsrDI 1062 GCAATG GCAATG(2/0) BsrGI 5753 TGTACA T^GTACA BssSI 3205 CTCGTG CACGAG(-5/-1) Bst1107I 4671 GTATAC GTA^TAC BstZ17I 4671 GTATAC GTA^TAC CelII 1057 GCTCAGC GC^T[ACGT]AGC Eam1105I 5218 GACCTGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FbaI 898 TGATCA T^GATCA FspI 3946 TGCGCA TGC^GCA HaeII 4000 GGCGCC [GA]GCGC^[CT] Hin1I 4000 GGCGCC G[GA]^CG[CT]C KasI 4000 GGCGCC G^GCGCC KpnI 3249 GGTACC GGTAC^C KspI 4128 CCGCGG CCGC^GG MamI 2004 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 991 TGGCCA TGG^CCA MroI 2582 TCCGGA T^CCGGA MscI 991 TGGCCA TGG^CCA NarI 4000 GGCGCC GG^CGCC PflMI 1761 CCACTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 4757 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 4383 AACGTT AA^CGTT SacII 4128 CCGCGG CCGC^GG SapI 1742 GCTCTTC GCTCTTC(1/4) SbfI 764 CCTGCAGG CCTGCA^GG SfoI 4000 GGCGCC GGC^GCC SnaBI 5732 TACGTA TAC^GTA SpeI 6110 ACTAGT A^CTAGT Sse8387I 764 CCTGCAGG CCTGCA^GG SspBI 5753 TGTACA T^GTACA SwaI 6033 ATTTAAAT ATTT^AAAT Van91I 1761 CCACTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 5293 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### Alw44I 907 GTGCAC G^TGCAC 4564 GTGCAC AlwI 4084 GATCC GGATC(4/5) 418 GGATC AocI 2090 CCTCAGG CC^T[ACGT]AGG 3853 CCTCAGG ApaLI 907 GTGCAC G^TGCAC 4564 GTGCAC BbvCI 1044 CCTCAGC CCTCAGC(-5/-2) 1013 GCTGAGG BglI 30 GCCATGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3698 GCCTGCCGGGC BsaWI 415 ACCGGA [AT]^CCGG[AT] 2582 TCCGGA BspHI 1606 TCATGA T^CATGA 1622 TCATGA Bsu36I 2090 CCTCAGG CC^T[ACGT]AGG 3853 CCTCAGG DraIII 3625 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG 5945 CACTTCGTG EaeI 991 TGGCCA [CT]^GGCC[GA] 3055 TGGCCG EarI 1743 CTCTTC CTCTTC(1/4) 537 GAAGAG Eco81I 2090 CCTCAGG CC^T[ACGT]AGG 3853 CCTCAGG EcoRI 2676 GAATTC G^AATTC 5026 GAATTC EcoT14I 11 CCTTGG C^C[AT][AT]GG 1517 CCTAGG HgaI 3268 GACGC GACGC(5/10) 3979 GCGTC Ksp632I 1743 CTCTTC CTCTTC(1/4) 537 GAAGAG MfeI 2797 CAATTG C^AATTG 5401 CAATTG MunI 2797 CAATTG C^AATTG 5401 CAATTG NdeI 5757 CATATG CA^TATG 5871 CATATG PpuMI 892 AGGACCT [GA]G^G[AT]CC[CT] 2560 AGGTCCT PvuII 2826 CAGCTG CAG^CTG 3289 CAGCTG RcaI 1606 TCATGA T^CATGA 1622 TCATGA SacI 647 GAGCTC GAGCT^C 1740 GAGCTC StyI 11 CCTTGG C^C[AT][AT]GG 1517 CCTAGG ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflII AluI AlwNI ApoI AseI AsnI Asp700I AspHI AvaII BanI BanII BbsI BbvI BciVI BcnI BfaI BfrI BglII BmyI BpmI BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EcoO109I EcoRII EcoT22I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PleI PshBI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI