##################################
# NO CUT ENZYMES (   56 enzymes) #
##################################

AatII       AgeI        Aor51HI     AscI        AspI        
BamHI       BbrPI       BsiEI       BsiWI       BspDI       
BssHII      BstBI       BstEII      BstPI       ClaI        
CpoI        DrdI        EagI        EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRV       FseI        
HpaI        MluI        NaeI        NcoI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PvuI        RsrII       SalI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SphI        StuI        Tth111I     XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

Acc65I       3249  GGTACC               G^GTACC
AccIII       2582  TCCGGA               T^CCGGA
AclI         4383  AACGTT               AA^CGTT
AcyI         4000  GGCGCC               G[GA]^CG[CT]C
AflIII       6271  ACATGT               A^C[GA][CT]GT
AhdI         5218  GACCTGTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI         4105  GGGCCC               GGGCC^C
Asp718I      3249  GGTACC               G^GTACC
AspEI        5218  GACCTGTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         3511  CCCGAG               C^[CT]CG[GA]G
AviII        3946  TGCGCA               TGC^GCA
AvrII        1517  CCTAGG               C^CTAGG
BalI          991  TGGCCA               TGG^CCA
BbeI         4000  GGCGCC               GGCGC^C
BcgI          739  CGACCATCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          898  TGATCA               T^GATCA
BlnI         1517  CCTAGG               C^CTAGG
BlpI         1057  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1057  GCTCAGC              GC^T[ACGT]AGC
BsaBI        2004  GATCTGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        4000  GGCGCC               G[GA]^CG[CT]C
BseAI        2582  TCCGGA               T^CCGGA
BsmBI        3610  GAGACG               CGTCTC(1/5)
BsoBI        3511  CCCGAG               C^[CT]CG[GA]G
Bsp1407I     5753  TGTACA               T^GTACA
BspEI        2582  TCCGGA               T^CCGGA
BspLU11I     6271  ACATGT               A^CATGT
BsrDI        1062  GCAATG               GCAATG(2/0)
BsrGI        5753  TGTACA               T^GTACA
BssSI        3205  CTCGTG               CACGAG(-5/-1)
Bst1107I     4671  GTATAC               GTA^TAC
BstZ17I      4671  GTATAC               GTA^TAC
CelII        1057  GCTCAGC              GC^T[ACGT]AGC
Eam1105I     5218  GACCTGTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI          898  TGATCA               T^GATCA
FspI         3946  TGCGCA               TGC^GCA
HaeII        4000  GGCGCC               [GA]GCGC^[CT]
Hin1I        4000  GGCGCC               G[GA]^CG[CT]C
KasI         4000  GGCGCC               G^GCGCC
KpnI         3249  GGTACC               GGTAC^C
KspI         4128  CCGCGG               CCGC^GG
MamI         2004  GATCTGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         991  TGGCCA               TGG^CCA
MroI         2582  TCCGGA               T^CCGGA
MscI          991  TGGCCA               TGG^CCA
NarI         4000  GGCGCC               GG^CGCC
PflMI        1761  CCACTATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        4757  GACTGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     4383  AACGTT               AA^CGTT
SacII        4128  CCGCGG               CCGC^GG
SapI         1742  GCTCTTC              GCTCTTC(1/4)
SbfI          764  CCTGCAGG             CCTGCA^GG
SfoI         4000  GGCGCC               GGC^GCC
SnaBI        5732  TACGTA               TAC^GTA
SpeI         6110  ACTAGT               A^CTAGT
Sse8387I      764  CCTGCAGG             CCTGCA^GG
SspBI        5753  TGTACA               T^GTACA
SwaI         6033  ATTTAAAT             ATTT^AAAT
Van91I       1761  CCACTATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         5293  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

Alw44I        907  GTGCAC               G^TGCAC
             4564  GTGCAC              
AlwI         4084  GATCC                GGATC(4/5)
              418  GGATC               
AocI         2090  CCTCAGG              CC^T[ACGT]AGG
             3853  CCTCAGG             
ApaLI         907  GTGCAC               G^TGCAC
             4564  GTGCAC              
BbvCI        1044  CCTCAGC              CCTCAGC(-5/-2)
             1013  GCTGAGG             
BglI           30  GCCATGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3698  GCCTGCCGGGC         
BsaWI         415  ACCGGA               [AT]^CCGG[AT]
             2582  TCCGGA              
BspHI        1606  TCATGA               T^CATGA
             1622  TCATGA              
Bsu36I       2090  CCTCAGG              CC^T[ACGT]AGG
             3853  CCTCAGG             
DraIII       3625  CACGCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             5945  CACTTCGTG           
EaeI          991  TGGCCA               [CT]^GGCC[GA]
             3055  TGGCCG              
EarI         1743  CTCTTC               CTCTTC(1/4)
              537  GAAGAG              
Eco81I       2090  CCTCAGG              CC^T[ACGT]AGG
             3853  CCTCAGG             
EcoRI        2676  GAATTC               G^AATTC
             5026  GAATTC              
EcoT14I        11  CCTTGG               C^C[AT][AT]GG
             1517  CCTAGG              
HgaI         3268  GACGC                GACGC(5/10)
             3979  GCGTC               
Ksp632I      1743  CTCTTC               CTCTTC(1/4)
              537  GAAGAG              
MfeI         2797  CAATTG               C^AATTG
             5401  CAATTG              
MunI         2797  CAATTG               C^AATTG
             5401  CAATTG              
NdeI         5757  CATATG               CA^TATG
             5871  CATATG              
PpuMI         892  AGGACCT              [GA]G^G[AT]CC[CT]
             2560  AGGTCCT             
PvuII        2826  CAGCTG               CAG^CTG
             3289  CAGCTG              
RcaI         1606  TCATGA               T^CATGA
             1622  TCATGA              
SacI          647  GAGCTC               GAGCT^C
             1740  GAGCTC              
StyI           11  CCTTGG               C^C[AT][AT]GG
             1517  CCTAGG              


#########################################
# 3 OR MORE CUT ENZYMES (  117 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AflII       AluI        AlwNI       ApoI        AseI        
AsnI        Asp700I     AspHI       AvaII       BanI        
BanII       BbsI        BbvI        BciVI       BcnI        
BfaI        BfrI        BglII       BmyI        BpmI        
BpuAI       BsaAI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        Bsp1286I    BspMI       BsrBI       
BsrFI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraI        DraII       DsaI        EcoO109I    EcoRII      
EcoT22I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HincII      HindII      
HindIII     HinfI       HpaII       HphI        ItaI        
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NsiI        NspI        
PleI        PshBI       PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XcmI        
XhoII       XmnI