##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AccI AccIII AclI AgeI Aor51HI
AseI AsnI AspI BciVI BclI
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssSI
Bst1107I BstZ17I ClaI CpoI DraI
Eco47III EcoRI EcoRV FbaI FseI
HincII HindII HindIII HpaI MfeI
MluI MroI MunI PacI PflFI
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SalI SgrAI SnaBI
SpeI SspBI SspI SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AatII 4805 GACGTC GACGT^C
Acc65I 2999 GGTACC G^GTACC
AflIII 2168 ACGTGT A^C[GA][CT]GT
AhdI 2164 GACCACGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AscI 444 GGCGCGCC GG^CGCGCC
Asp718I 2999 GGTACC G^GTACC
AspEI 2164 GACCACGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1823 TGCGCA TGC^GCA
BcgI 1967 GCAGAGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 3858 AGATCT A^GATCT
BsaBI 285 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BstBI 3842 TTCGAA TT^CGAA
Eam1105I 2164 GACCACGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 1823 TGCGCA TGC^GCA
KpnI 2999 GGTACC GGTAC^C
KspI 137 CCGCGG CCGC^GG
MamI 285 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 1004 CATATG CA^TATG
NheI 211 GCTAGC G^CTAGC
NotI 1 GCGGCCGC GC^GGCCGC
NruI 2126 TCGCGA TCG^CGA
NspV 3842 TTCGAA TT^CGAA
PaeR7I 5587 CTCGAG C^TCGAG
PshAI 2250 GACTCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 137 CCGCGG CCGC^GG
SfiI 532 GGCCAGGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 3842 TTCGAA TT^CGAA
XhoI 5587 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 3228 AAATTC [GA]^AATT[CT]
3350 GAATTT
AflII 3344 CTTAAG C^TTAAG
5400 CTTAAG
ApoI 3228 AAATTC [GA]^AATT[CT]
3350 GAATTT
Asp700I 3329 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5187 GAAGCACTTC
AvrII 3014 CCTAGG C^CTAGG
3640 CCTAGG
BalI 1871 TGGCCA TGG^CCA
5324 TGGCCA
BfrI 3344 CTTAAG C^TTAAG
5400 CTTAAG
BlnI 3014 CCTAGG C^CTAGG
3640 CCTAGG
BlpI 501 GCTGAGC GC^T[ACGT]AGC
726 GCTGAGC
Bpu1102I 501 GCTGAGC GC^T[ACGT]AGC
726 GCTGAGC
BsmBI 1977 CGTCTC CGTCTC(1/5)
775 GAGACG
CelII 501 GCTGAGC GC^T[ACGT]AGC
726 GCTGAGC
DrdI 2591 GACTGTCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5583 GACACTCGAGTC
MluNI 1871 TGGCCA TGG^CCA
5324 TGGCCA
MscI 1871 TGGCCA TGG^CCA
5324 TGGCCA
MseI 3345 TTAA T^TAA
5401 TTAA
SbfI 1893 CCTGCAGG CCTGCA^GG
5475 CCTGCAGG
ScaI 4743 AGTACT AGT^ACT
5404 AGTACT
SexAI 200 ACCTGGT A^CC[AT]GGT
704 ACCTGGT
Sse8387I 1893 CCTGCAGG CCTGCA^GG
5475 CCTGCAGG
TfiI 2433 GATTC G^A[AT]TC
3692 GATTC
Tru9I 3345 TTAA T^TAA
5401 TTAA
XmnI 3329 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5187 GAAGCACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 151 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AspHI AvaI AvaII BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssHII BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EagI
EarI EclXI Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NsiI NspI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI SphI StuI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI