################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## Acc65I AclI AflII AflIII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AvrII BamHI BclI BfrI BlnI BsaBI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrGI Bst1107I BstBI BstZ17I ClaI DraI Eco47III EcoRV EcoT22I FbaI HindIII HpaI KpnI MamI MluI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI ScaI SfuI SgrAI SpeI SphI SspBI SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AatII 3344 GACGTC GACGT^C AccI 437 GTCGAC GT^[AC][GT]AC AccIII 729 TCCGGA T^CCGGA Asp700I 91 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 488 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 4311 TGCGCA TGC^GCA BbrPI 3273 CACGTG CAC^GTG BcgI 384 CGAGGCACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1175 GGATAC GTATCC(6/5) BseAI 729 TCCGGA T^CCGGA BspEI 729 TCCGGA T^CCGGA BsrDI 4225 CATTGC GCAATG(2/0) BssHII 1444 GCGCGC G^CGCGC BstXI 3078 CCATGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CpoI 3876 CGGACCG CG^G[AT]CCG DraIII 699 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRI 1759 GAATTC G^AATTC FspI 4311 TGCGCA TGC^GCA HincII 437 GTCGAC GT[CT]^[GA]AC HindII 437 GTCGAC GT[CT]^[GA]AC KspI 854 CCGCGG CCGC^GG MfeI 1463 CAATTG C^AATTG MroI 729 TCCGGA T^CCGGA MseI 4332 TTAA T^TAA MunI 1463 CAATTG C^AATTG PflFI 488 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 3273 CACGTG CAC^GTG PmlI 3273 CACGTG CAC^GTG RsrII 3876 CGGACCG CG^G[AT]CCG SacII 854 CCGCGG CCGC^GG SalI 437 GTCGAC G^TCGAC SexAI 700 ACCAGGT A^CC[AT]GGT SfiI 2461 GGCCCTGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SnaBI 5212 TACGTA TAC^GTA Tru9I 4332 TTAA T^TAA Tth111I 488 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 91 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 1724 AAATTT [GA]^AATT[CT] 1759 GAATTC AhdI 2156 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2512 GACTGCACGTC AocI 1225 CCTGAGG CC^T[ACGT]AGG 4685 CCTGAGG ApoI 1724 AAATTT [GA]^AATT[CT] 1759 GAATTC AspEI 2156 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2512 GACTGCACGTC BsaAI 3273 CACGTG [CT]AC^GT[GA] 5212 TACGTA BsaWI 729 TCCGGA [AT]^CCGG[AT] 2283 TCCGGT BstEII 1135 GGTCACC G^GT[ACGT]ACC 3938 GGTAACC BstPI 1135 GGTCACC G^GT[ACGT]ACC 3938 GGTAACC Bsu36I 1225 CCTGAGG CC^T[ACGT]AGG 4685 CCTGAGG DrdI 1127 GACCTGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1142 GACATCATCGTC Eam1105I 2156 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2512 GACTGCACGTC Eco81I 1225 CCTGAGG CC^T[ACGT]AGG 4685 CCTGAGG EcoO65I 1135 GGTCACC G^GT[ACGT]ACC 3938 GGTAACC FseI 258 GGCCGGCC GGCCGG^CC 4471 GGCCGGCC MslI 157 CATGTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 3506 CATGAGCGTG NcoI 2799 CCATGG C^CATGG 3078 CCATGG SapI 4007 GAAGAGC GCTCTTC(1/4) 142 GCTCTTC SbfI 3517 CCTGCAGG CCTGCA^GG 4069 CCTGCAGG Sse8387I 3517 CCTGCAGG CCTGCA^GG 4069 CCTGCAGG StuI 1014 AGGCCT AGG^CCT 5277 AGGCCT TfiI 3802 GAATC G^A[AT]TC 5193 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 134 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbeI BbsI BbvCI BbvI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI