##################################
# NO CUT ENZYMES (   65 enzymes) #
##################################

Acc65I      AclI        AflII       AflIII      AgeI        
Alw44I      Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AvrII       BamHI       BclI        
BfrI        BlnI        BsaBI       BsiWI       BsmI        
Bsp1407I    BspDI       BspHI       BspLU11I    BsrGI       
Bst1107I    BstBI       BstZ17I     ClaI        DraI        
Eco47III    EcoRV       EcoT22I     FbaI        HindIII     
HpaI        KpnI        MamI        MluI        NdeI        
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
ScaI        SfuI        SgrAI       SpeI        SphI        
SspBI       SspI        SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AatII        3344  GACGTC               GACGT^C
AccI          437  GTCGAC               GT^[AC][GT]AC
AccIII        729  TCCGGA               T^CCGGA
Asp700I        91  GAACTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          488  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        4311  TGCGCA               TGC^GCA
BbrPI        3273  CACGTG               CAC^GTG
BcgI          384  CGAGGCACCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1175  GGATAC               GTATCC(6/5)
BseAI         729  TCCGGA               T^CCGGA
BspEI         729  TCCGGA               T^CCGGA
BsrDI        4225  CATTGC               GCAATG(2/0)
BssHII       1444  GCGCGC               G^CGCGC
BstXI        3078  CCATGGACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI         3876  CGGACCG              CG^G[AT]CCG
DraIII        699  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoRI        1759  GAATTC               G^AATTC
FspI         4311  TGCGCA               TGC^GCA
HincII        437  GTCGAC               GT[CT]^[GA]AC
HindII        437  GTCGAC               GT[CT]^[GA]AC
KspI          854  CCGCGG               CCGC^GG
MfeI         1463  CAATTG               C^AATTG
MroI          729  TCCGGA               T^CCGGA
MseI         4332  TTAA                 T^TAA
MunI         1463  CAATTG               C^AATTG
PflFI         488  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        3273  CACGTG               CAC^GTG
PmlI         3273  CACGTG               CAC^GTG
RsrII        3876  CGGACCG              CG^G[AT]CCG
SacII         854  CCGCGG               CCGC^GG
SalI          437  GTCGAC               G^TCGAC
SexAI         700  ACCAGGT              A^CC[AT]GGT
SfiI         2461  GGCCCTGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI        5212  TACGTA               TAC^GTA
Tru9I        4332  TTAA                 T^TAA
Tth111I       488  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI           91  GAACTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AcsI         1724  AAATTT               [GA]^AATT[CT]
             1759  GAATTC              
AhdI         2156  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2512  GACTGCACGTC         
AocI         1225  CCTGAGG              CC^T[ACGT]AGG
             4685  CCTGAGG             
ApoI         1724  AAATTT               [GA]^AATT[CT]
             1759  GAATTC              
AspEI        2156  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2512  GACTGCACGTC         
BsaAI        3273  CACGTG               [CT]AC^GT[GA]
             5212  TACGTA              
BsaWI         729  TCCGGA               [AT]^CCGG[AT]
             2283  TCCGGT              
BstEII       1135  GGTCACC              G^GT[ACGT]ACC
             3938  GGTAACC             
BstPI        1135  GGTCACC              G^GT[ACGT]ACC
             3938  GGTAACC             
Bsu36I       1225  CCTGAGG              CC^T[ACGT]AGG
             4685  CCTGAGG             
DrdI         1127  GACCTGAAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1142  GACATCATCGTC        
Eam1105I     2156  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2512  GACTGCACGTC         
Eco81I       1225  CCTGAGG              CC^T[ACGT]AGG
             4685  CCTGAGG             
EcoO65I      1135  GGTCACC              G^GT[ACGT]ACC
             3938  GGTAACC             
FseI          258  GGCCGGCC             GGCCGG^CC
             4471  GGCCGGCC            
MslI          157  CATGTTCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             3506  CATGAGCGTG          
NcoI         2799  CCATGG               C^CATGG
             3078  CCATGG              
SapI         4007  GAAGAGC              GCTCTTC(1/4)
              142  GCTCTTC             
SbfI         3517  CCTGCAGG             CCTGCA^GG
             4069  CCTGCAGG            
Sse8387I     3517  CCTGCAGG             CCTGCA^GG
             4069  CCTGCAGG            
StuI         1014  AGGCCT               AGG^CCT
             5277  AGGCCT              
TfiI         3802  GAATC                G^A[AT]TC
             5193  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (  134 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbeI        
BbsI        BbvCI       BbvI        BcnI        BfaI        
BglI        BglII       BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrBI       BsrFI       BsrI        
BssKI       BssSI       BstAPI      BstF5I      BstNI       
BstUI       BstYI       Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EagI        EarI        EclXI       
Eco52I      EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        KasI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NdeII       NgoMIV      
NlaIII      NlaIV       PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfoI        
SmaI        SmlI        StyI        TaqI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI        XhoII       XmaCI       XmaI