##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
Acc65I AclI AcsI AflII ApoI
AseI AsnI Asp718I BamHI BciVI
BclI BfrI BsaBI BsiWI BspDI
BsrDI Bst1107I BstZ17I ClaI CpoI
EcoRI EcoRV FbaI HpaI KpnI
MamI MfeI MluI MunI NdeI
NheI NotI NruI PacI PmeI
PshBI Psp1406I PvuI RsrII SalI
SbfI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 2044 GACGTC GACGT^C
AccI 4232 GTCTAC GT^[AC][GT]AC
AgeI 1974 ACCGGT A^CCGGT
AhdI 269 GACTGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4307 GTGCAC G^TGCAC
ApaLI 4307 GTGCAC G^TGCAC
AscI 1059 GGCGCGCC GG^CGCGCC
AspEI 269 GACTGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3731 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 5403 CCTAGG C^CTAGG
BglII 1357 AGATCT A^GATCT
BlnI 5403 CCTAGG C^CTAGG
BlpI 4939 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4939 GCTGAGC GC^T[ACGT]AGC
BsmBI 5653 CGTCTC CGTCTC(1/5)
Bsp1407I 4032 TGTACA T^GTACA
BsrGI 4032 TGTACA T^GTACA
BstAPI 3361 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3621 TTCGAA TT^CGAA
BstEII 3829 GGTCACC G^GT[ACGT]ACC
BstPI 3829 GGTCACC G^GT[ACGT]ACC
CelII 4939 GCTGAGC GC^T[ACGT]AGC
DraI 5397 TTTAAA TTT^AAA
DrdI 1318 GACTCAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 269 GACTGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3829 GGTCACC G^GT[ACGT]ACC
EcoT22I 5940 ATGCAT ATGCA^T
HindIII 3661 AAGCTT A^AGCTT
NsiI 5940 ATGCAT ATGCA^T
NspV 3621 TTCGAA TT^CGAA
PflFI 3731 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1974 ACCGGT A^CCGGT
SfiI 364 GGCCGTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 3621 TTCGAA TT^CGAA
SspBI 4032 TGTACA T^GTACA
TfiI 1412 GATTC G^A[AT]TC
Tsp509I 5479 AATT ^AATT
Tth111I 3731 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 1396 TCCGGA T^CCGGA
3972 TCCGGA
AflIII 4065 ACATGT A^C[GA][CT]GT
6011 ACATGT
AocI 165 CCTGAGG CC^T[ACGT]AGG
5812 CCTCAGG
Asp700I 1930 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4270 GAAGGTGTTC
BbrPI 2526 CACGTG CAC^GTG
5922 CACGTG
BseAI 1396 TCCGGA T^CCGGA
3972 TCCGGA
BsmI 3913 GAATGC GAATGC(1/-1)
5942 GCATTC
BspEI 1396 TCCGGA T^CCGGA
3972 TCCGGA
BspLU11I 4065 ACATGT A^CATGT
6011 ACATGT
Bsu36I 165 CCTGAGG CC^T[ACGT]AGG
5812 CCTCAGG
Eco81I 165 CCTGAGG CC^T[ACGT]AGG
5812 CCTCAGG
FseI 561 GGCCGGCC GGCCGG^CC
3323 GGCCGGCC
HincII 4484 GTCAAC GT[CT]^[GA]AC
5448 GTCAAC
HindII 4484 GTCAAC GT[CT]^[GA]AC
5448 GTCAAC
KspI 515 CCGCGG CCGC^GG
567 CCGCGG
MroI 1396 TCCGGA T^CCGGA
3972 TCCGGA
MseI 721 TTAA T^TAA
5398 TTAA
NspI 4065 ACATGT [GA]CATG^[CT]
6011 ACATGT
PmaCI 2526 CACGTG CAC^GTG
5922 CACGTG
PmlI 2526 CACGTG CAC^GTG
5922 CACGTG
PshAI 449 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
695 GACTCCAGTC
SacII 515 CCGCGG CCGC^GG
567 CCGCGG
ScaI 3882 AGTACT AGT^ACT
4566 AGTACT
SexAI 2377 ACCAGGT A^CC[AT]GGT
3732 ACCTGGT
Tru9I 721 TTAA T^TAA
5398 TTAA
XbaI 1797 TCTAGA T^CTAGA
5515 TCTAGA
XmnI 1930 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4270 GAAGGTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 145 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI Aor51HI ApaI AspHI
AvaI AvaII AviII BalI BanI
BanII BbeI BbsI BbvCI BbvI
BcgI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrBI
BsrFI BsrI BssHII BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EagI EarI EclXI Eco47III Eco52I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV PaeR7I
PflMI PleI PpuMI PstI PvuII
RcaI RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StuI StyI TaqI
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoI XhoII XmaCI XmaI