################################## # NO CUT ENZYMES ( 48 enzymes) # ################################## Acc65I AclI AcsI AflII ApoI AseI AsnI Asp718I BamHI BciVI BclI BfrI BsaBI BsiWI BspDI BsrDI Bst1107I BstZ17I ClaI CpoI EcoRI EcoRV FbaI HpaI KpnI MamI MfeI MluI MunI NdeI NheI NotI NruI PacI PmeI PshBI Psp1406I PvuI RsrII SalI SbfI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AatII 2044 GACGTC GACGT^C AccI 4232 GTCTAC GT^[AC][GT]AC AgeI 1974 ACCGGT A^CCGGT AhdI 269 GACTGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 4307 GTGCAC G^TGCAC ApaLI 4307 GTGCAC G^TGCAC AscI 1059 GGCGCGCC GG^CGCGCC AspEI 269 GACTGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 3731 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 5403 CCTAGG C^CTAGG BglII 1357 AGATCT A^GATCT BlnI 5403 CCTAGG C^CTAGG BlpI 4939 GCTGAGC GC^T[ACGT]AGC Bpu1102I 4939 GCTGAGC GC^T[ACGT]AGC BsmBI 5653 CGTCTC CGTCTC(1/5) Bsp1407I 4032 TGTACA T^GTACA BsrGI 4032 TGTACA T^GTACA BstAPI 3361 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 3621 TTCGAA TT^CGAA BstEII 3829 GGTCACC G^GT[ACGT]ACC BstPI 3829 GGTCACC G^GT[ACGT]ACC CelII 4939 GCTGAGC GC^T[ACGT]AGC DraI 5397 TTTAAA TTT^AAA DrdI 1318 GACTCAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 269 GACTGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 3829 GGTCACC G^GT[ACGT]ACC EcoT22I 5940 ATGCAT ATGCA^T HindIII 3661 AAGCTT A^AGCTT NsiI 5940 ATGCAT ATGCA^T NspV 3621 TTCGAA TT^CGAA PflFI 3731 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 1974 ACCGGT A^CCGGT SfiI 364 GGCCGTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 3621 TTCGAA TT^CGAA SspBI 4032 TGTACA T^GTACA TfiI 1412 GATTC G^A[AT]TC Tsp509I 5479 AATT ^AATT Tth111I 3731 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccIII 1396 TCCGGA T^CCGGA 3972 TCCGGA AflIII 4065 ACATGT A^C[GA][CT]GT 6011 ACATGT AocI 165 CCTGAGG CC^T[ACGT]AGG 5812 CCTCAGG Asp700I 1930 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4270 GAAGGTGTTC BbrPI 2526 CACGTG CAC^GTG 5922 CACGTG BseAI 1396 TCCGGA T^CCGGA 3972 TCCGGA BsmI 3913 GAATGC GAATGC(1/-1) 5942 GCATTC BspEI 1396 TCCGGA T^CCGGA 3972 TCCGGA BspLU11I 4065 ACATGT A^CATGT 6011 ACATGT Bsu36I 165 CCTGAGG CC^T[ACGT]AGG 5812 CCTCAGG Eco81I 165 CCTGAGG CC^T[ACGT]AGG 5812 CCTCAGG FseI 561 GGCCGGCC GGCCGG^CC 3323 GGCCGGCC HincII 4484 GTCAAC GT[CT]^[GA]AC 5448 GTCAAC HindII 4484 GTCAAC GT[CT]^[GA]AC 5448 GTCAAC KspI 515 CCGCGG CCGC^GG 567 CCGCGG MroI 1396 TCCGGA T^CCGGA 3972 TCCGGA MseI 721 TTAA T^TAA 5398 TTAA NspI 4065 ACATGT [GA]CATG^[CT] 6011 ACATGT PmaCI 2526 CACGTG CAC^GTG 5922 CACGTG PmlI 2526 CACGTG CAC^GTG 5922 CACGTG PshAI 449 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 695 GACTCCAGTC SacII 515 CCGCGG CCGC^GG 567 CCGCGG ScaI 3882 AGTACT AGT^ACT 4566 AGTACT SexAI 2377 ACCAGGT A^CC[AT]GGT 3732 ACCTGGT Tru9I 721 TTAA T^TAA 5398 TTAA XbaI 1797 TCTAGA T^CTAGA 5515 TCTAGA XmnI 1930 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4270 GAAGGTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 145 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI Aor51HI ApaI AspHI AvaI AvaII AviII BalI BanI BanII BbeI BbsI BbvCI BbvI BcgI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrBI BsrFI BsrI BssHII BssKI BssSI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EagI EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV PaeR7I PflMI PleI PpuMI PstI PvuII RcaI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI StuI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoI XhoII XmaCI XmaI