##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
Acc65I AccII AccIII AclI Aor51HI
AscI Asp718I AviII BbeI BcgI
BclI BlpI Bpu1102I BseAI BsiWI
BsmBI BsmI BspEI BspHI BssHII
BssSI Bst1107I BstBI BstUI BstZ17I
CelII CpoI DrdI EagI EclXI
Eco47III Eco52I EcoT22I FbaI FseI
FspI HaeII HpaI KasI KpnI
KspI MluI MroI MspA1I MvnI
NarI NdeI NotI NruI NsiI
NspV PacI PaeR7I PmeI PshAI
Psp1406I PvuII RcaI RsrII SacII
SapI SbfI SfiI SfoI SfuI
SnaBI SphI Sse8387I SspI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AflII 3687 CTTAAG C^TTAAG
AflIII 3573 ACATGT A^C[GA][CT]GT
AhdI 2559 GACTGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 659 GTGCAC G^TGCAC
ApaLI 659 GTGCAC G^TGCAC
AspEI 2559 GACTGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1754 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1382 GGATCC G^GATCC
BbrPI 3159 CACGTG CAC^GTG
BbsI 3224 GTCTTC GAAGAC(2/6)
BbvCI 32 CCTCAGC CCTCAGC(-5/-2)
BfrI 3687 CTTAAG C^TTAAG
BglI 3685 GCCTTAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 3224 GTCTTC GAAGAC(2/6)
BsaBI 3985 GATTAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1072 CGATCG CG[GA][CT]^CG
BspDI 1070 ATCGAT AT^CGAT
BspLU11I 3573 ACATGT A^CATGT
BstAPI 1482 GCAATGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 827 GGTCACC G^GT[ACGT]ACC
BstPI 827 GGTCACC G^GT[ACGT]ACC
ClaI 1070 ATCGAT AT^CGAT
DraIII 411 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1786 CCATGG C^C[GA][CT]GG
Eam1105I 2559 GACTGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 827 GGTCACC G^GT[ACGT]ACC
EcoRI 1847 GAATTC G^AATTC
EcoRV 267 GATATC GAT^ATC
HgaI 1276 GCGTC GACGC(5/10)
MamI 3985 GATTAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1786 CCATGG C^CATGG
NspI 3573 ACATGT [GA]CATG^[CT]
PflFI 1754 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3159 CACGTG CAC^GTG
PmlI 3159 CACGTG CAC^GTG
PstI 3292 CTGCAG CTGCA^G
PvuI 1072 CGATCG CGAT^CG
SacI 119 GAGCTC GAGCT^C
SalI 1365 GTCGAC G^TCGAC
ScaI 2297 AGTACT AGT^ACT
SgrAI 806 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 3049 CCCGGG CCC^GGG
Tth111I 1754 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3914 TCTAGA T^CTAGA
XmaCI 3049 CCCGGG C^CCGGG
XmaI 3049 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AatII 52 GACGTC GACGT^C
617 GACGTC
AcyI 52 GACGTC G[GA]^CG[CT]C
617 GACGTC
AgeI 1839 ACCGGT A^CCGGT
2708 ACCGGT
AseI 2144 ATTAAT AT^TAAT
3986 ATTAAT
AsnI 2144 ATTAAT AT^TAAT
3986 ATTAAT
AvrII 2912 CCTAGG C^CTAGG
3320 CCTAGG
BciVI 1544 GTATCC GTATCC(6/5)
1740 GTATCC
BlnI 2912 CCTAGG C^CTAGG
3320 CCTAGG
BsaAI 1008 TACGTG [CT]AC^GT[GA]
3159 CACGTG
BsaHI 52 GACGTC G[GA]^CG[CT]C
617 GACGTC
BsaWI 1839 ACCGGT [AT]^CCGG[AT]
2708 ACCGGT
Bsp1407I 195 TGTACA T^GTACA
441 TGTACA
BsrGI 195 TGTACA T^GTACA
441 TGTACA
EcoNI 490 CCTGGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1549 CCTCCCTCAGG
Hin1I 52 GACGTC G[GA]^CG[CT]C
617 GACGTC
HincII 1365 GTCGAC GT[CT]^[GA]AC
4150 GTCAAC
HindII 1365 GTCGAC GT[CT]^[GA]AC
4150 GTCAAC
HindIII 978 AAGCTT A^AGCTT
2313 AAGCTT
MfeI 1300 CAATTG C^AATTG
3830 CAATTG
MunI 1300 CAATTG C^AATTG
3830 CAATTG
NaeI 807 GCCGGC GCC^GGC
1596 GCCGGC
NgoMIV 807 GCCGGC G^CCGGC
1596 GCCGGC
NheI 1887 GCTAGC G^CTAGC
3952 GCTAGC
PflMI 976 CCAAGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2834 CCAGGGCATGG
PinAI 1839 ACCGGT A^CCGGT
2708 ACCGGT
PshBI 2144 ATTAAT AT^TAAT
3986 ATTAAT
SpeI 2954 ACTAGT A^CTAGT
4097 ACTAGT
SspBI 195 TGTACA T^GTACA
441 TGTACA
StuI 80 AGGCCT AGG^CCT
1716 AGGCCT
TaqI 1071 TCGA T^CGA
1366 TCGA
TthHB8I 1071 TCGA T^CGA
1366 TCGA
Van91I 976 CCAAGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2834 CCAGGGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BglII BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI XhoII XmnI