##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AflII AgeI Alw44I Aor51HI ApaLI
AscI AviII AvrII BbrPI BciVI
BfrI BlnI BsiWI BssHII Bst1107I
BstAPI BstZ17I Eco47III EcoRV EcoT22I
FseI FspI HindIII HpaI KspI
MluI NdeI NheI NotI NruI
NsiI PacI PaeR7I PinAI PmaCI
PmlI PshAI PvuI SacII SalI
SapI SbfI SfiI SgrAI SmlI
SnaBI SpeI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AatII 3337 GACGTC GACGT^C
AccIII 2240 TCCGGA T^CCGGA
AclI 631 AACGTT AA^CGTT
AhdI 1990 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 7675 ATTAAT AT^TAAT
AsnI 7675 ATTAAT AT^TAAT
AspEI 1990 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 129 GGATCC G^GATCC
BbvCI 4557 CCTCAGC CCTCAGC(-5/-2)
BseAI 2240 TCCGGA T^CCGGA
BsmBI 6745 GAGACG CGTCTC(1/5)
BspEI 2240 TCCGGA T^CCGGA
BsrBI 6042 GAGCGG CCGCTC(-3/-3)
BssSI 7532 CACGAG CACGAG(-5/-1)
CpoI 6831 CGGACCG CG^G[AT]CCG
DraIII 1086 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1990 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
MfeI 2688 CAATTG C^AATTG
MroI 2240 TCCGGA T^CCGGA
MunI 2688 CAATTG C^AATTG
NaeI 5463 GCCGGC GCC^GGC
NgoMIV 5463 GCCGGC G^CCGGC
PmeI 1944 GTTTAAAC GTTT^AAAC
PshBI 7675 ATTAAT AT^TAAT
Psp1406I 631 AACGTT AA^CGTT
RsrII 6831 CGGACCG CG^G[AT]CCG
SmaI 755 CCCGGG CCC^GGG
SphI 3041 GCATGC GCATG^C
XmaCI 755 CCCGGG C^CCGGG
XmaI 755 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
BbeI 3572 GGCGCC GGCGC^C
3806 GGCGCC
BcgI 4562 GCAAACACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
6014 GCACCACCATCG
BsaBI 3459 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4909 GATGGCTATC
BsmI 169 GAATGC GAATGC(1/-1)
466 GAATGC
Bsp1407I 2951 TGTACA T^GTACA
7433 TGTACA
BspDI 31 ATCGAT AT^CGAT
1849 ATCGAT
BspLU11I 6896 ACATGT A^CATGT
7645 ACATGT
BsrFI 607 ACCGGC [GA]^CCGG[CT]
5463 GCCGGC
BsrGI 2951 TGTACA T^GTACA
7433 TGTACA
BstBI 2423 TTCGAA TT^CGAA
4942 TTCGAA
BstEII 3267 GGTAACC G^GT[ACGT]ACC
5850 GGTGACC
BstPI 3267 GGTAACC G^GT[ACGT]ACC
5850 GGTGACC
Cfr10I 607 ACCGGC [GA]^CCGG[CT]
5463 GCCGGC
ClaI 31 ATCGAT AT^CGAT
1849 ATCGAT
EcoO65I 3267 GGTAACC G^GT[ACGT]ACC
5850 GGTGACC
KasI 3572 GGCGCC G^GCGCC
3806 GGCGCC
MamI 3459 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4909 GATGGCTATC
NarI 3572 GGCGCC GG^CGCC
3806 GGCGCC
NspV 2423 TTCGAA TT^CGAA
4942 TTCGAA
SacI 1291 GAGCTC GAGCT^C
1326 GAGCTC
SexAI 3731 ACCAGGT A^CC[AT]GGT
5999 ACCTGGT
SfoI 3572 GGCGCC GGC^GCC
3806 GGCGCC
SfuI 2423 TTCGAA TT^CGAA
4942 TTCGAA
SspBI 2951 TGTACA T^GTACA
7433 TGTACA
XbaI 1330 TCTAGA T^CTAGA
2403 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 153 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AcyI AfaI AflIII AluI AlwI
AlwNI AocI ApaI ApoI Asp700I
Asp718I AspHI AspI AvaI AvaII
BalI BanI BanII BbsI BbvI
BclI BcnI BfaI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrDI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DrdI
DsaI EaeI EagI EarI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoRI
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII XmnI