##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AscI Asp718I AspI AvrII BbeI
BglI BlnI BsaHI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BsrBI
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I ClaI CpoI DrdI EagI
EclXI Eco52I EcoO65I FauI FseI
Hin1I KasI KpnI KspI MluI
MroI NaeI NarI NgoMIV NotI
NruI PacI PflFI PmeI Psp1406I
PvuI RsrII SacII SbfI SexAI
SfiI SfoI SnaBI Sse8387I Tth111I
XcmI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccII 5983 CGCG CG^CG
AflII 4661 CTTAAG C^TTAAG
AgeI 4913 ACCGGT A^CCGGT
AhdI 1979 GACAGTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4359 GTGCAC G^TGCAC
Aor51HI 7155 AGCGCT AGC^GCT
ApaI 4099 GGGCCC GGGCC^C
ApaLI 4359 GTGCAC G^TGCAC
AspEI 1979 GACAGTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 6455 GGATCC G^GATCC
BbrPI 6293 CACGTG CAC^GTG
BbvCI 5988 CCTCAGC CCTCAGC(-5/-2)
BciVI 246 GGATAC GTATCC(6/5)
BclI 5590 TGATCA T^GATCA
BfrI 4661 CTTAAG C^TTAAG
BlpI 6558 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 6558 GCTAAGC GC^T[ACGT]AGC
BsaAI 6293 CACGTG [CT]AC^GT[GA]
BsaI 3198 GGTCTC GGTCTC(1/5)
BsaWI 4913 ACCGGT [AT]^CCGG[AT]
Bsp1407I 350 TGTACA T^GTACA
BspHI 3869 TCATGA T^CATGA
BspMI 1728 GCAGGT ACCTGC(4/8)
BsrFI 4913 ACCGGT [GA]^CCGG[CT]
BsrGI 350 TGTACA T^GTACA
BstUI 5983 CGCG CG^CG
CelII 6558 GCTAAGC GC^T[ACGT]AGC
Cfr10I 4913 ACCGGT [GA]^CCGG[CT]
DraIII 1252 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1979 GACAGTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 7155 AGCGCT AGC^GCT
EcoNI 585 CCTTCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1078 GATATC GAT^ATC
FbaI 5590 TGATCA T^GATCA
MfeI 481 CAATTG C^AATTG
MspA1I 1653 CAGCTG C[AC]G^C[GT]G
MunI 481 CAATTG C^AATTG
MvnI 5983 CGCG CG^CG
PaeR7I 1852 CTCGAG C^TCGAG
PinAI 4913 ACCGGT A^CCGGT
PmaCI 6293 CACGTG CAC^GTG
PmlI 6293 CACGTG CAC^GTG
PvuII 1653 CAGCTG CAG^CTG
RcaI 3869 TCATGA T^CATGA
SacI 4571 GAGCTC GAGCT^C
SalI 1023 GTCGAC G^TCGAC
SgrAI 4912 CACCGGTG C[GA]^CCGG[CT]G
SmaI 5413 CCCGGG CCC^GGG
SpeI 2232 ACTAGT A^CTAGT
SspBI 350 TGTACA T^GTACA
XbaI 522 TCTAGA T^CTAGA
XhoI 1852 CTCGAG C^TCGAG
XmaCI 5413 CCCGGG C^CCGGG
XmaI 5413 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AocI 3193 CCTTAGG CC^T[ACGT]AGG
3669 CCTCAGG
AviII 6880 TGCGCA TGC^GCA
7196 TGCGCA
BalI 2120 TGGCCA TGG^CCA
7572 TGGCCA
BcgI 87 CGACCTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
6302 CGATCATGGTGC
BseRI 7110 CTCCTC GAGGAG(10/8)
7113 CTCCTC
BspLU11I 1613 ACATGT A^CATGT
3655 ACATGT
BstAPI 1899 GCATTACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4230 GCAAGTATTGC
Bsu36I 3193 CCTTAGG CC^T[ACGT]AGG
3669 CCTCAGG
EaeI 2120 TGGCCA [CT]^GGCC[GA]
7572 TGGCCA
Eco81I 3193 CCTTAGG CC^T[ACGT]AGG
3669 CCTCAGG
EcoRI 5199 GAATTC G^AATTC
6697 GAATTC
FspI 6880 TGCGCA TGC^GCA
7196 TGCGCA
HaeII 3976 AGCGCC [GA]GCGC^[CT]
7155 AGCGCT
HindIII 370 AAGCTT A^AGCTT
2563 AAGCTT
HpaI 6108 GTTAAC GTT^AAC
7427 GTTAAC
MluNI 2120 TGGCCA TGG^CCA
7572 TGGCCA
MscI 2120 TGGCCA TGG^CCA
7572 TGGCCA
NdeI 324 CATATG CA^TATG
5699 CATATG
NheI 2789 GCTAGC G^CTAGC
2849 GCTAGC
PflMI 1251 CCACTAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3957 CCAGATATTGG
PpuMI 2470 GGGACCC [GA]G^G[AT]CC[CT]
5470 AGGTCCT
PshAI 3483 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4030 GACGAAAGTC
SapI 5127 GCTCTTC GCTCTTC(1/4)
6626 GCTCTTC
SwaI 7308 ATTTAAAT ATTT^AAAT
7371 ATTTAAAT
Van91I 1251 CCACTAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3957 CCAGATATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaBI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BsrDI BsrI BssKI BstBI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EarI EcoO109I EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NsiI
NspI NspV PleI PshBI PstI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SfuI SmlI SphI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI