##################################
# NO CUT ENZYMES ( 40 enzymes) #
##################################
AflII AgeI AscI AspI AvrII
BfrI BglI BlnI BsiEI BsiWI
BspDI BspHI BssHII BssSI BstBI
ClaI CpoI DrdI EagI EclXI
Eco52I EcoRI MluI NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PvuI RcaI RsrII
SfiI SfuI SnaBI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 4740 GACGTC GACGT^C
AclI 4096 AACGTT AA^CGTT
AflIII 1892 ACATGT A^C[GA][CT]GT
AhdI 7181 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4180 GTGCAC G^TGCAC
Aor51HI 379 AGCGCT AGC^GCT
ApaI 4393 GGGCCC GGGCC^C
ApaLI 4180 GTGCAC G^TGCAC
AspEI 7181 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3254 TGCGCA TGC^GCA
BbeI 7443 GGCGCC GGCGC^C
BbrPI 1258 CACGTG CAC^GTG
BcgI 6544 GCAATTTTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 502 GTATCC GTATCC(6/5)
BlpI 2322 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2322 GCTCAGC GC^T[ACGT]AGC
BspLU11I 1892 ACATGT A^CATGT
Bst1107I 6900 GTATAC GTA^TAC
BstEII 3486 GGTGACC G^GT[ACGT]ACC
BstPI 3486 GGTGACC G^GT[ACGT]ACC
BstZ17I 6900 GTATAC GTA^TAC
CelII 2322 GCTCAGC GC^T[ACGT]AGC
Eam1105I 7181 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 379 AGCGCT AGC^GCT
EcoO65I 3486 GGTGACC G^GT[ACGT]ACC
EcoRV 3544 GATATC GAT^ATC
FseI 5261 GGCCGGCC GGCCGG^CC
FspI 3254 TGCGCA TGC^GCA
HpaI 5722 GTTAAC GTT^AAC
KasI 7443 GGCGCC G^GCGCC
NarI 7443 GGCGCC GG^CGCC
NcoI 4066 CCATGG C^CATGG
PmaCI 1258 CACGTG CAC^GTG
PmlI 1258 CACGTG CAC^GTG
Psp1406I 4096 AACGTT AA^CGTT
SalI 2885 GTCGAC G^TCGAC
SapI 1350 GCTCTTC GCTCTTC(1/4)
SbfI 451 CCTGCAGG CCTGCA^GG
SexAI 4873 ACCAGGT A^CC[AT]GGT
SfoI 7443 GGCGCC GGC^GCC
SgrAI 2573 CGCCGGTG C[GA]^CCGG[CT]G
SpeI 898 ACTAGT A^CTAGT
Sse8387I 451 CCTGCAGG CCTGCA^GG
SwaI 6588 ATTTAAAT ATTT^AAAT
XbaI 1155 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 4877 GGTACC G^GTACC
5735 GGTACC
AccI 2885 GTCGAC GT^[AC][GT]AC
6900 GTATAC
AccIII 2188 TCCGGA T^CCGGA
3716 TCCGGA
Asp718I 4877 GGTACC G^GTACC
5735 GGTACC
BamHI 1668 GGATCC G^GATCC
4019 GGATCC
BglII 4536 AGATCT A^GATCT
5060 AGATCT
BsaAI 1258 CACGTG [CT]AC^GT[GA]
3415 TACGTG
BseAI 2188 TCCGGA T^CCGGA
3716 TCCGGA
BspEI 2188 TCCGGA T^CCGGA
3716 TCCGGA
BsrDI 5094 CATTGC GCAATG(2/0)
6708 CATTGC
EcoNI 3396 CCTGTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3591 CCTGTTGAAGG
EcoT22I 3060 ATGCAT ATGCA^T
3754 ATGCAT
KpnI 4877 GGTACC GGTAC^C
5735 GGTACC
KspI 2946 CCGCGG CCGC^GG
4175 CCGCGG
MfeI 1373 CAATTG C^AATTG
7895 CAATTG
MroI 2188 TCCGGA T^CCGGA
3716 TCCGGA
MunI 1373 CAATTG C^AATTG
7895 CAATTG
NaeI 1864 GCCGGC GCC^GGC
5262 GCCGGC
NdeI 3751 CATATG CA^TATG
5171 CATATG
NgoMIV 1864 GCCGGC G^CCGGC
5262 GCCGGC
NsiI 3060 ATGCAT ATGCA^T
3754 ATGCAT
PshAI 313 GACATTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4816 GACCAAGGTC
SacI 2314 GAGCTC GAGCT^C
3775 GAGCTC
SacII 2946 CCGCGG CCGC^GG
4175 CCGCGG
StuI 810 AGGCCT AGG^CCT
7271 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
AseI AsnI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BclI BcnI BfaI
BmyI BpmI BpuAI BsaBI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspMI BsrBI BsrFI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PshBI PstI
PvuII RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmaI SmlI
SphI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI XmnI