################################## # NO CUT ENZYMES ( 54 enzymes) # ################################## AatII AclI AcyI AgeI AhdI AscI AseI AsnI AspEI BbeI BciVI BsaHI BsiEI BsmBI BspDI BspLU11I BssHII ClaI CpoI EagI Eam1105I EclXI Eco52I FseI Hin1I HpaI KasI KspI MfeI MluI MunI NarI NdeI NheI NotI NruI PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SgrAI SnaBI Sse8387I SwaI XhoI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### Acc65I 628 GGTACC G^GTACC AflII 3946 CTTAAG C^TTAAG AocI 2196 CCTGAGG CC^T[ACGT]AGG Aor51HI 7465 AGCGCT AGC^GCT Asp718I 628 GGTACC G^GTACC AviII 1975 TGCGCA TGC^GCA AvrII 8115 CCTAGG C^CTAGG BfrI 3946 CTTAAG C^TTAAG BlnI 8115 CCTAGG C^CTAGG BsaBI 276 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiWI 2425 CGTACG C^GTACG BstBI 1159 TTCGAA TT^CGAA Bsu36I 2196 CCTGAGG CC^T[ACGT]AGG DraI 7504 TTTAAA TTT^AAA DraIII 8088 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 6148 GACTATGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 7465 AGCGCT AGC^GCT Eco81I 2196 CCTGAGG CC^T[ACGT]AGG EcoRV 4546 GATATC GAT^ATC EcoT22I 2850 ATGCAT ATGCA^T FspI 1975 TGCGCA TGC^GCA KpnI 628 GGTACC GGTAC^C MamI 276 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 461 GCCGGC GCC^GGC NgoMIV 461 GCCGGC G^CCGGC NsiI 2850 ATGCAT ATGCA^T NspV 1159 TTCGAA TT^CGAA PshAI 1047 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC ScaI 1529 AGTACT AGT^ACT SexAI 3239 ACCAGGT A^CC[AT]GGT SfuI 1159 TTCGAA TT^CGAA SmaI 2444 CCCGGG CCC^GGG SpeI 2149 ACTAGT A^CTAGT XmaCI 2444 CCCGGG C^CCGGG XmaI 2444 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccIII 1310 TCCGGA T^CCGGA 2990 TCCGGA AspI 1321 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC 6601 GACCACGTC BamHI 729 GGATCC G^GATCC 7085 GGATCC BglII 2069 AGATCT A^GATCT 2663 AGATCT BseAI 1310 TCCGGA T^CCGGA 2990 TCCGGA BspEI 1310 TCCGGA T^CCGGA 2990 TCCGGA BsrBI 6172 GAGCGG CCGCTC(-3/-3) 6403 GAGCGG Bst1107I 1466 GTATAC GTA^TAC 8222 GTATAC BstEII 636 GGTGACC G^GT[ACGT]ACC 8333 GGTGACC BstPI 636 GGTGACC G^GT[ACGT]ACC 8333 GGTGACC BstZ17I 1466 GTATAC GTA^TAC 8222 GTATAC EcoO65I 636 GGTGACC G^GT[ACGT]ACC 8333 GGTGACC HaeII 2835 GGCGCT [GA]GCGC^[CT] 7465 AGCGCT HgaI 4057 GACGC GACGC(5/10) 7915 GACGC HindIII 2298 AAGCTT A^AGCTT 5498 AAGCTT MroI 1310 TCCGGA T^CCGGA 2990 TCCGGA PflFI 1321 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC 6601 GACCACGTC SspI 3632 AATATT AAT^ATT 3893 AATATT Tth111I 1321 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC 6601 GACCACGTC ######################################### # 3 OR MORE CUT ENZYMES ( 150 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI Alw44I AlwI AlwNI ApaI ApaLI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbrPI BbsI BbvCI BbvI BcgI BclI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspHI BspMI BsrDI BsrFI BsrGI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RcaI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XcmI XhoII XmnI