##################################
# NO CUT ENZYMES (   54 enzymes) #
##################################

AatII       AclI        AcyI        AgeI        AhdI        
AscI        AseI        AsnI        AspEI       BbeI        
BciVI       BsaHI       BsiEI       BsmBI       BspDI       
BspLU11I    BssHII      ClaI        CpoI        EagI        
Eam1105I    EclXI       Eco52I      FseI        Hin1I       
HpaI        KasI        KspI        MfeI        MluI        
MunI        NarI        NdeI        NheI        NotI        
NruI        PacI        PaeR7I      PinAI       PmeI        
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        SfiI        SfoI        SgrAI       
SnaBI       Sse8387I    SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

Acc65I        628  GGTACC               G^GTACC
AflII        3946  CTTAAG               C^TTAAG
AocI         2196  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      7465  AGCGCT               AGC^GCT
Asp718I       628  GGTACC               G^GTACC
AviII        1975  TGCGCA               TGC^GCA
AvrII        8115  CCTAGG               C^CTAGG
BfrI         3946  CTTAAG               C^TTAAG
BlnI         8115  CCTAGG               C^CTAGG
BsaBI         276  GATGCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI        2425  CGTACG               C^GTACG
BstBI        1159  TTCGAA               TT^CGAA
Bsu36I       2196  CCTGAGG              CC^T[ACGT]AGG
DraI         7504  TTTAAA               TTT^AAA
DraIII       8088  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         6148  GACTATGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     7465  AGCGCT               AGC^GCT
Eco81I       2196  CCTGAGG              CC^T[ACGT]AGG
EcoRV        4546  GATATC               GAT^ATC
EcoT22I      2850  ATGCAT               ATGCA^T
FspI         1975  TGCGCA               TGC^GCA
KpnI          628  GGTACC               GGTAC^C
MamI          276  GATGCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI          461  GCCGGC               GCC^GGC
NgoMIV        461  GCCGGC               G^CCGGC
NsiI         2850  ATGCAT               ATGCA^T
NspV         1159  TTCGAA               TT^CGAA
PshAI        1047  GACCAAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI         1529  AGTACT               AGT^ACT
SexAI        3239  ACCAGGT              A^CC[AT]GGT
SfuI         1159  TTCGAA               TT^CGAA
SmaI         2444  CCCGGG               CCC^GGG
SpeI         2149  ACTAGT               A^CTAGT
XmaCI        2444  CCCGGG               C^CCGGG
XmaI         2444  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AccIII       1310  TCCGGA               T^CCGGA
             2990  TCCGGA              
AspI         1321  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             6601  GACCACGTC           
BamHI         729  GGATCC               G^GATCC
             7085  GGATCC              
BglII        2069  AGATCT               A^GATCT
             2663  AGATCT              
BseAI        1310  TCCGGA               T^CCGGA
             2990  TCCGGA              
BspEI        1310  TCCGGA               T^CCGGA
             2990  TCCGGA              
BsrBI        6172  GAGCGG               CCGCTC(-3/-3)
             6403  GAGCGG              
Bst1107I     1466  GTATAC               GTA^TAC
             8222  GTATAC              
BstEII        636  GGTGACC              G^GT[ACGT]ACC
             8333  GGTGACC             
BstPI         636  GGTGACC              G^GT[ACGT]ACC
             8333  GGTGACC             
BstZ17I      1466  GTATAC               GTA^TAC
             8222  GTATAC              
EcoO65I       636  GGTGACC              G^GT[ACGT]ACC
             8333  GGTGACC             
HaeII        2835  GGCGCT               [GA]GCGC^[CT]
             7465  AGCGCT              
HgaI         4057  GACGC                GACGC(5/10)
             7915  GACGC               
HindIII      2298  AAGCTT               A^AGCTT
             5498  AAGCTT              
MroI         1310  TCCGGA               T^CCGGA
             2990  TCCGGA              
PflFI        1321  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             6601  GACCACGTC           
SspI         3632  AATATT               AAT^ATT
             3893  AATATT              
Tth111I      1321  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             6601  GACCACGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  150 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AflIII      AluI        Alw44I      AlwI        AlwNI       
ApaI        ApaLI       ApoI        Asp700I     AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbrPI       BbsI        BbvCI       BbvI        BcgI        
BclI        BcnI        BfaI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaI        BsaJI       BsaWI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspHI       
BspMI       BsrDI       BsrFI       BsrGI       BsrI        
BssKI       BssSI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoNI       
EcoO109I    EcoRI       EcoRII      EcoT14I     FauI        
FbaI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PmaCI       PmlI        
PpuMI       PstI        PvuII       RcaI        RsaI        
SacI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SphI        SspBI       
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XbaI        XcmI        XhoII       XmnI