################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## Acc65I AccIII AgeI AhdI AocI Aor51HI AscI Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbvCI BcgI BlnI BsaWI BseAI BsiWI BsmBI BspDI BspEI BsrBI BssHII Bsu36I ClaI CpoI Eam1105I Eco47III Eco81I FseI FspI KasI KpnI KspI MluNI MroI MscI NarI NotI NruI PacI PflFI PinAI PmeI PshAI PvuI RsrII SacII SapI SbfI SfiI SfoI SgrAI SmaI SnaBI Sse8387I SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AatII 3 GACGTC GACGT^C AcyI 3 GACGTC G[GA]^CG[CT]C Alw44I 5942 GTGCAC G^TGCAC ApaI 2619 GGGCCC GGGCC^C ApaLI 5942 GTGCAC G^TGCAC BanI 2359 GGCACC G^G[CT][GA]CC BbrPI 512 CACGTG CAC^GTG BclI 2166 TGATCA T^GATCA BsaBI 6234 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 3 GACGTC G[GA]^CG[CT]C BsiEI 2473 CGGCCG CG[GA][CT]^CG BsmI 2840 GCATTC GAATGC(1/-1) Bsp1407I 5787 TGTACA T^GTACA BsrGI 5787 TGTACA T^GTACA BstBI 2286 TTCGAA TT^CGAA BstXI 1458 CCAAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EagI 2473 CGGCCG C^GGCCG EclXI 2473 CGGCCG C^GGCCG Eco52I 2473 CGGCCG C^GGCCG EcoRI 4643 GAATTC G^AATTC EcoRV 581 GATATC GAT^ATC FbaI 2166 TGATCA T^GATCA HgaI 182 GACGC GACGC(5/10) Hin1I 3 GACGTC G[GA]^CG[CT]C MamI 6234 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 183 ACGCGT A^CGCGT NdeI 3362 CATATG CA^TATG NheI 694 GCTAGC G^CTAGC NspV 2286 TTCGAA TT^CGAA PflMI 13 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 512 CACGTG CAC^GTG PmlI 512 CACGTG CAC^GTG SalI 1955 GTCGAC G^TCGAC ScaI 5038 AGTACT AGT^ACT SfuI 2286 TTCGAA TT^CGAA SpeI 5905 ACTAGT A^CTAGT SspBI 5787 TGTACA T^GTACA Van91I 13 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AclI 2174 AACGTT AA^CGTT 3878 AACGTT BglI 117 GCCCCTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 881 GCCATGCTGGC BsgI 3301 CTGCAC GTGCAG(16/14) 5152 GTGCAG BspMI 957 ACCTGC ACCTGC(4/8) 2400 GCAGGT BsrFI 113 GCCGGC [GA]^CCGG[CT] 254 GCCGGC BssSI 622 CACGAG CACGAG(-5/-1) 3275 CACGAG BstAPI 362 GCAGAATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3303 GCACTGTTTGC BstEII 2403 GGTCACC G^GT[ACGT]ACC 4391 GGTTACC BstPI 2403 GGTCACC G^GT[ACGT]ACC 4391 GGTTACC Cfr10I 113 GCCGGC [GA]^CCGG[CT] 254 GCCGGC DraIII 2442 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG 5945 CACTGTGTG DrdI 101 GACCAGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1568 GACATTCAAGTC EcoO65I 2403 GGTCACC G^GT[ACGT]ACC 4391 GGTTACC FauI 160 CCCGC CCCGC(4/6) 2623 CCCGC HindIII 1762 AAGCTT A^AGCTT 3381 AAGCTT HpaI 4181 GTTAAC GTT^AAC 4472 GTTAAC MfeI 2060 CAATTG C^AATTG 4922 CAATTG MunI 2060 CAATTG C^AATTG 4922 CAATTG NaeI 113 GCCGGC GCC^GGC 254 GCCGGC NcoI 2049 CCATGG C^CATGG 2986 CCATGG NgoMIV 113 GCCGGC G^CCGGC 254 GCCGGC PaeR7I 272 CTCGAG C^TCGAG 303 CTCGAG Psp1406I 2174 AACGTT AA^CGTT 3878 AACGTT PvuII 104 CAGCTG CAG^CTG 1960 CAGCTG SexAI 1548 ACCAGGT A^CC[AT]GGT 3034 ACCAGGT SphI 5157 GCATGC GCATG^C 5396 GCATGC StuI 2609 AGGCCT AGG^CCT 5168 AGGCCT XcmI 13 CCAGGACCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4019 CCAGAAGCCAACTGG XhoI 272 CTCGAG C^TCGAG 303 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 128 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflII AflIII AluI AlwI AlwNI ApoI AseI AsnI Asp700I AspHI AvaI AvaII BanII BbsI BbvI BciVI BcnI BfaI BfrI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspLU11I BsrDI BsrI BssKI Bst1107I BstF5I BstNI BstUI BstYI BstZ17I Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PleI PpuMI PshBI PstI RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII XmnI