##################################
# NO CUT ENZYMES (   63 enzymes) #
##################################

Acc65I      AccIII      AgeI        AhdI        AocI        
Aor51HI     AscI        Asp718I     AspEI       AspI        
AviII       AvrII       BalI        BamHI       BbeI        
BbvCI       BcgI        BlnI        BsaWI       BseAI       
BsiWI       BsmBI       BspDI       BspEI       BsrBI       
BssHII      Bsu36I      ClaI        CpoI        Eam1105I    
Eco47III    Eco81I      FseI        FspI        KasI        
KpnI        KspI        MluNI       MroI        MscI        
NarI        NotI        NruI        PacI        PflFI       
PinAI       PmeI        PshAI       PvuI        RsrII       
SacII       SapI        SbfI        SfiI        SfoI        
SgrAI       SmaI        SnaBI       Sse8387I    SwaI        
Tth111I     XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AatII           3  GACGTC               GACGT^C
AcyI            3  GACGTC               G[GA]^CG[CT]C
Alw44I       5942  GTGCAC               G^TGCAC
ApaI         2619  GGGCCC               GGGCC^C
ApaLI        5942  GTGCAC               G^TGCAC
BanI         2359  GGCACC               G^G[CT][GA]CC
BbrPI         512  CACGTG               CAC^GTG
BclI         2166  TGATCA               T^GATCA
BsaBI        6234  GATCTGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI           3  GACGTC               G[GA]^CG[CT]C
BsiEI        2473  CGGCCG               CG[GA][CT]^CG
BsmI         2840  GCATTC               GAATGC(1/-1)
Bsp1407I     5787  TGTACA               T^GTACA
BsrGI        5787  TGTACA               T^GTACA
BstBI        2286  TTCGAA               TT^CGAA
BstXI        1458  CCAAAAAACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI         2473  CGGCCG               C^GGCCG
EclXI        2473  CGGCCG               C^GGCCG
Eco52I       2473  CGGCCG               C^GGCCG
EcoRI        4643  GAATTC               G^AATTC
EcoRV         581  GATATC               GAT^ATC
FbaI         2166  TGATCA               T^GATCA
HgaI          182  GACGC                GACGC(5/10)
Hin1I           3  GACGTC               G[GA]^CG[CT]C
MamI         6234  GATCTGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI          183  ACGCGT               A^CGCGT
NdeI         3362  CATATG               CA^TATG
NheI          694  GCTAGC               G^CTAGC
NspV         2286  TTCGAA               TT^CGAA
PflMI          13  CCAGGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         512  CACGTG               CAC^GTG
PmlI          512  CACGTG               CAC^GTG
SalI         1955  GTCGAC               G^TCGAC
ScaI         5038  AGTACT               AGT^ACT
SfuI         2286  TTCGAA               TT^CGAA
SpeI         5905  ACTAGT               A^CTAGT
SspBI        5787  TGTACA               T^GTACA
Van91I         13  CCAGGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AclI         2174  AACGTT               AA^CGTT
             3878  AACGTT              
BglI          117  GCCCCTCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              881  GCCATGCTGGC         
BsgI         3301  CTGCAC               GTGCAG(16/14)
             5152  GTGCAG              
BspMI         957  ACCTGC               ACCTGC(4/8)
             2400  GCAGGT              
BsrFI         113  GCCGGC               [GA]^CCGG[CT]
              254  GCCGGC              
BssSI         622  CACGAG               CACGAG(-5/-1)
             3275  CACGAG              
BstAPI        362  GCAGAATATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3303  GCACTGTTTGC         
BstEII       2403  GGTCACC              G^GT[ACGT]ACC
             4391  GGTTACC             
BstPI        2403  GGTCACC              G^GT[ACGT]ACC
             4391  GGTTACC             
Cfr10I        113  GCCGGC               [GA]^CCGG[CT]
              254  GCCGGC              
DraIII       2442  CACTAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             5945  CACTGTGTG           
DrdI          101  GACCAGCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1568  GACATTCAAGTC        
EcoO65I      2403  GGTCACC              G^GT[ACGT]ACC
             4391  GGTTACC             
FauI          160  CCCGC                CCCGC(4/6)
             2623  CCCGC               
HindIII      1762  AAGCTT               A^AGCTT
             3381  AAGCTT              
HpaI         4181  GTTAAC               GTT^AAC
             4472  GTTAAC              
MfeI         2060  CAATTG               C^AATTG
             4922  CAATTG              
MunI         2060  CAATTG               C^AATTG
             4922  CAATTG              
NaeI          113  GCCGGC               GCC^GGC
              254  GCCGGC              
NcoI         2049  CCATGG               C^CATGG
             2986  CCATGG              
NgoMIV        113  GCCGGC               G^CCGGC
              254  GCCGGC              
PaeR7I        272  CTCGAG               C^TCGAG
              303  CTCGAG              
Psp1406I     2174  AACGTT               AA^CGTT
             3878  AACGTT              
PvuII         104  CAGCTG               CAG^CTG
             1960  CAGCTG              
SexAI        1548  ACCAGGT              A^CC[AT]GGT
             3034  ACCAGGT             
SphI         5157  GCATGC               GCATG^C
             5396  GCATGC              
StuI         2609  AGGCCT               AGG^CCT
             5168  AGGCCT              
XcmI           13  CCAGGACCTGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4019  CCAGAAGCCAACTGG     
XhoI          272  CTCGAG               C^TCGAG
              303  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  128 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AflII       AflIII      AluI        AlwI        AlwNI       
ApoI        AseI        AsnI        Asp700I     AspHI       
AvaI        AvaII       BanII       BbsI        BbvI        
BciVI       BcnI        BfaI        BfrI        BglII       
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspHI       BspLU11I    BsrDI       BsrI        
BssKI       Bst1107I    BstF5I      BstNI       BstUI       
BstYI       BstZ17I     Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
DsaI        EaeI        EarI        EcoNI       EcoO109I    
EcoRII      EcoT14I     EcoT22I     Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NsiI        NspI        PleI        PpuMI       PshBI       
PstI        RcaI        RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XbaI        XhoII       XmnI