##################################
# NO CUT ENZYMES (   59 enzymes) #
##################################

Acc65I      AccIII      AclI        AocI        Aor51HI     
AscI        AseI        AsnI        Asp718I     AviII       
AvrII       BbeI        BbrPI       BciVI       BlnI        
BsaAI       BseAI       BsiWI       BspEI       BspHI       
BspLU11I    BssHII      Bst1107I    BstBI       BstZ17I     
Bsu36I      CpoI        DraI        Eco47III    Eco81I      
EcoRV       FseI        FspI        HpaI        KasI        
KpnI        MluI        MroI        NarI        NdeI        
NheI        NruI        NspV        PacI        PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RcaI        RsrII       ScaI        SfoI        SfuI        
SnaBI       SpeI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AatII        6195  GACGTC               GACGT^C
AflII        1358  CTTAAG               C^TTAAG
AflIII       2682  ACACGT               A^C[GA][CT]GT
AgeI         3186  ACCGGT               A^CCGGT
AhdI         5386  GACCAGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI         3981  GGGCCC               GGGCC^C
Asp700I      1876  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        5386  GACCAGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI        5065  GGATCC               G^GATCC
BcgI         5125  CGAAGCTGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI         1542  CTAG                 C^TAG
BfrI         1358  CTTAAG               C^TTAAG
BglII        5208  AGATCT               A^GATCT
BsaBI        1321  GATGGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I     6400  TGTACA               T^GTACA
BsrBI         415  CCGCTC               CCGCTC(-3/-3)
BsrGI        6400  TGTACA               T^GTACA
BstEII       4540  GGTGACC              G^GT[ACGT]ACC
BstPI        4540  GGTGACC              G^GT[ACGT]ACC
Eam1105I     5386  GACCAGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1819  GAAGAG               CTCTTC(1/4)
EcoNI         472  CCTGAAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      4540  GGTGACC              G^GT[ACGT]ACC
HindIII      1308  AAGCTT               A^AGCTT
Ksp632I      1819  GAAGAG               CTCTTC(1/4)
KspI         1614  CCGCGG               CCGC^GG
MaeI         1542  CTAG                 C^TAG
MamI         1321  GATGGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI         5006  GCCGGC               GCC^GGC
NgoMIV       5006  GCCGGC               G^CCGGC
NotI         1616  GCGGCCGC             GC^GGCCGC
PinAI        3186  ACCGGT               A^CCGGT
SacII        1614  CCGCGG               CCGC^GG
SalI         3728  GTCGAC               G^TCGAC
SapI         1819  GAAGAGC              GCTCTTC(1/4)
SbfI         3271  CCTGCAGG             CCTGCA^GG
SgrAI        5005  CGCCGGCG             C[GA]^CCGG[CT]G
Sse8387I     3271  CCTGCAGG             CCTGCA^GG
SspBI        6400  TGTACA               T^GTACA
SspI         6205  AATATT               AAT^ATT
XmnI         1876  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccI         3728  GTCGAC               GT^[AC][GT]AC
             4764  GTAGAC              
AccII         660  CGCG                 CG^CG
             1615  CGCG                
Alw44I       4903  GTGCAC               G^TGCAC
             6058  GTGCAC              
ApaLI        4903  GTGCAC               G^TGCAC
             6058  GTGCAC              
BanI         3858  GGCACC               G^G[CT][GA]CC
             4601  GGCACC              
BsaWI        2426  ACCGGA               [AT]^CCGG[AT]
             3186  ACCGGT              
BsiEI         568  CGGCCG               CG[GA][CT]^CG
             1617  CGGCCG              
BspMI         478  GCAGGT               ACCTGC(4/8)
             5464  GCAGGT              
BsrFI        3186  ACCGGT               [GA]^CCGG[CT]
             5006  GCCGGC              
BssSI        3244  CACGAG               CACGAG(-5/-1)
             3930  CTCGTG              
BstUI         660  CGCG                 CG^CG
             1615  CGCG                
Cfr10I       3186  ACCGGT               [GA]^CCGG[CT]
             5006  GCCGGC              
DraIII        301  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2230  CACTCTGTG           
DrdI         5075  GACCCAACTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5579  GACAATCTTGTC        
EagI          568  CGGCCG               C^GGCCG
             1617  CGGCCG              
EclXI         568  CGGCCG               C^GGCCG
             1617  CGGCCG              
Eco52I        568  CGGCCG               C^GGCCG
             1617  CGGCCG              
EcoT22I      4108  ATGCAT               ATGCA^T
             4630  ATGCAT              
MfeI         2667  CAATTG               C^AATTG
             4623  CAATTG              
MunI         2667  CAATTG               C^AATTG
             4623  CAATTG              
MvnI          660  CGCG                 CG^CG
             1615  CGCG                
NsiI         4108  ATGCAT               ATGCA^T
             4630  ATGCAT              
PaeR7I       1644  CTCGAG               C^TCGAG
             2288  CTCGAG              
SexAI        2793  ACCTGGT              A^CC[AT]GGT
             3065  ACCTGGT             
SphI         2609  GCATGC               GCATG^C
             4106  GCATGC              
XhoI         1644  CTCGAG               C^TCGAG
             2288  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  132 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApoI        AspHI       AspI        
AvaI        AvaII       BalI        BanII       BbsI        
BbvCI       BbvI        BclI        BcnI        BglI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspDI       
BsrDI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstXI       BstYI       Cac8I       CelII       
CfoI        Cfr13I      ClaI        DdeI        DpnII       
DraII       DsaI        EaeI        EcoO109I    EcoRI       
EcoRII      EcoT14I     FauI        FbaI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PflFI       
PflMI       PleI        PpuMI       PshAI       PstI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfiI        SmaI        
SmlI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Tth111I     TthHB8I     Van91I      XcmI        XhoII       
XmaCI       XmaI