##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccIII AflII AgeI AhdI
Aor51HI AscI AspEI AspI AviII
BamHI BcnI BfrI BglI BseAI
BsiEI BsiWI BspDI BspEI BsrFI
BssHII BstAPI BstEII BstPI Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
FseI FspI MfeI MluI MroI
MunI NaeI NciI NdeI NgoMIV
NotI NruI PacI PflFI PflMI
PinAI PmeI PvuI RsrII SalI
SbfI SfiI SgrAI SmaI SnaBI
Sse8387I SwaI Tth111I Van91I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AclI 7164 AACGTT AA^CGTT
Alw44I 7211 GTGCAC G^TGCAC
ApaLI 7211 GTGCAC G^TGCAC
Asp700I 4964 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 747 CTCGAG C^[CT]CG[GA]G
AvrII 212 CCTAGG C^CTAGG
BalI 9887 TGGCCA TGG^CCA
BbeI 62 GGCGCC GGCGC^C
BcgI 1102 CGATTTTTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 212 CCTAGG C^CTAGG
BsaWI 3371 ACCGGA [AT]^CCGG[AT]
BsmBI 3191 GAGACG CGTCTC(1/5)
BsoBI 747 CTCGAG C^[CT]CG[GA]G
BssSI 3567 CACGAG CACGAG(-5/-1)
BstBI 1718 TTCGAA TT^CGAA
EaeI 9887 TGGCCA [CT]^GGCC[GA]
EcoRI 1936 GAATTC G^AATTC
HgaI 2752 GACGC GACGC(5/10)
HpaI 4976 GTTAAC GTT^AAC
KasI 62 GGCGCC G^GCGCC
KspI 150 CCGCGG CCGC^GG
MluNI 9887 TGGCCA TGG^CCA
MscI 9887 TGGCCA TGG^CCA
NarI 62 GGCGCC GG^CGCC
NheI 3730 GCTAGC G^CTAGC
NspV 1718 TTCGAA TT^CGAA
PaeR7I 747 CTCGAG C^TCGAG
PpuMI 1474 GGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 7164 AACGTT AA^CGTT
PvuII 8792 CAGCTG CAG^CTG
SacI 6592 GAGCTC GAGCT^C
SacII 150 CCGCGG CCGC^GG
SexAI 5935 ACCAGGT A^CC[AT]GGT
SfoI 62 GGCGCC GGC^GCC
SfuI 1718 TTCGAA TT^CGAA
SphI 7370 GCATGC GCATG^C
XcmI 5592 CCAAGGCCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 747 CTCGAG C^TCGAG
XmnI 4964 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 2590 GGTACC G^GTACC
5939 GGTACC
AccII 151 CGCG CG^CG
863 CGCG
AcyI 62 GGCGCC G[GA]^CG[CT]C
2752 GACGCC
ApaI 6647 GGGCCC GGGCC^C
7806 GGGCCC
Asp718I 2590 GGTACC G^GTACC
5939 GGTACC
BbrPI 1970 CACGTG CAC^GTG
8718 CACGTG
BbvCI 1757 GCTGAGG CCTCAGC(-5/-2)
3587 GCTGAGG
BciVI 3140 GTATCC GTATCC(6/5)
10375 GTATCC
BsaHI 62 GGCGCC G[GA]^CG[CT]C
2752 GACGCC
BspMI 1363 GCAGGT ACCTGC(4/8)
2153 GCAGGT
Bst1107I 6754 GTATAC GTA^TAC
10693 GTATAC
BstUI 151 CGCG CG^CG
863 CGCG
BstZ17I 6754 GTATAC GTA^TAC
10693 GTATAC
HaeII 62 GGCGCC [GA]GCGC^[CT]
1086 AGCGCC
HapII 119 CCGG C^CGG
3372 CCGG
Hin1I 62 GGCGCC G[GA]^CG[CT]C
2752 GACGCC
HindIII 4485 AAGCTT A^AGCTT
8027 AAGCTT
HpaII 119 CCGG C^CGG
3372 CCGG
KpnI 2590 GGTACC GGTAC^C
5939 GGTACC
MspA1I 150 CCGCGG C[AC]G^C[GT]G
8792 CAGCTG
MspI 119 CCGG C^CGG
3372 CCGG
MvnI 151 CGCG CG^CG
863 CGCG
NcoI 914 CCATGG C^CATGG
7041 CCATGG
PmaCI 1970 CACGTG CAC^GTG
8718 CACGTG
PmlI 1970 CACGTG CAC^GTG
8718 CACGTG
PshAI 99 GACGGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
649 GACTCCAGTC
PstI 2078 CTGCAG CTGCA^G
5165 CTGCAG
SpeI 8438 ACTAGT A^CTAGT
8864 ACTAGT
StuI 50 AGGCCT AGG^CCT
1520 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AseI AsnI AspHI AvaII BanI
BanII BbsI BbvI BclI BfaI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspHI BspLU11I BsrBI BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EarI Eco81I EcoNI EcoO109I
EcoRII EcoRV EcoT14I EcoT22I FauI
FbaI Fnu4HI FokI HaeIII HhaI
HinP1I HincII HindII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV NsiI NspI PleI
PshBI RcaI RsaI SapI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XhoII