##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
ApaI AscI Asp718I AviII BamHI
BbrPI BciVI BclI BfrI BglI
BsiEI BsiWI BspDI BspHI BssHII
BstBI ClaI CpoI EagI EclXI
Eco52I EcoRV FbaI FseI FspI
HpaI KpnI KspI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 268 TCCGGA T^CCGGA
AocI 3502 CCTAAGG CC^T[ACGT]AGG
Aor51HI 868 AGCGCT AGC^GCT
AvrII 2194 CCTAGG C^CTAGG
BbeI 711 GGCGCC GGCGC^C
BcgI 5853 CGAGGGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 2194 CCTAGG C^CTAGG
BlpI 6379 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 6379 GCTGAGC GC^T[ACGT]AGC
BsaAI 5200 CACGTA [CT]AC^GT[GA]
BseAI 268 TCCGGA T^CCGGA
BspEI 268 TCCGGA T^CCGGA
BsrBI 4462 CCGCTC CCGCTC(-3/-3)
Bst1107I 7302 GTATAC GTA^TAC
BstZ17I 7302 GTATAC GTA^TAC
Bsu36I 3502 CCTAAGG CC^T[ACGT]AGG
CelII 6379 GCTGAGC GC^T[ACGT]AGC
Eco47III 868 AGCGCT AGC^GCT
Eco81I 3502 CCTAAGG CC^T[ACGT]AGG
KasI 711 GGCGCC G^GCGCC
MfeI 649 CAATTG C^AATTG
MluI 6408 ACGCGT A^CGCGT
MroI 268 TCCGGA T^CCGGA
MunI 649 CAATTG C^AATTG
NarI 711 GGCGCC GG^CGCC
PpuMI 3853 AGGTCCT [GA]G^G[AT]CC[CT]
SacI 5486 GAGCTC GAGCT^C
SexAI 2155 ACCAGGT A^CC[AT]GGT
SfoI 711 GGCGCC GGC^GCC
SmaI 1807 CCCGGG CCC^GGG
XmaCI 1807 CCCGGG C^CCGGG
XmaI 1807 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccII 6409 CGCG CG^CG
6431 CGCG
AcyI 711 GGCGCC G[GA]^CG[CT]C
3544 GACGCC
AhdI 60 GACTTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1122 GACGAGCTGTC
Alw44I 4382 GTGCAC G^TGCAC
4814 GTGCAC
ApaLI 4382 GTGCAC G^TGCAC
4814 GTGCAC
AseI 4704 ATTAAT AT^TAAT
5148 ATTAAT
AsnI 4704 ATTAAT AT^TAAT
5148 ATTAAT
AspEI 60 GACTTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1122 GACGAGCTGTC
AspI 1281 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2746 GACTGAGTC
BsaBI 2722 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
7309 GATTGTCATC
BsaHI 711 GGCGCC G[GA]^CG[CT]C
3544 GACGCC
BsaWI 64 TCCGGT [AT]^CCGG[AT]
268 TCCGGA
BsmBI 1300 CGTCTC CGTCTC(1/5)
4076 GAGACG
BssSI 1450 CTCGTG CACGAG(-5/-1)
3022 CTCGTG
BstAPI 5711 GCAAGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5772 GCACACCCTGC
BstEII 1155 GGTTACC G^GT[ACGT]ACC
6183 GGTAACC
BstPI 1155 GGTTACC G^GT[ACGT]ACC
6183 GGTAACC
BstUI 6409 CGCG CG^CG
6431 CGCG
BstXI 3277 CCATGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6644 CCAATTTACTGG
DsaI 1427 CCATGG C^C[GA][CT]GG
4136 CCATGG
Eam1105I 60 GACTTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1122 GACGAGCTGTC
EcoNI 219 CCTGTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3416 CCTAAAACAGG
EcoO65I 1155 GGTTACC G^GT[ACGT]ACC
6183 GGTAACC
EcoRI 2381 GAATTC G^AATTC
5917 GAATTC
EcoT22I 7088 ATGCAT ATGCA^T
7283 ATGCAT
Hin1I 711 GGCGCC G[GA]^CG[CT]C
3544 GACGCC
MamI 2722 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
7309 GATTGTCATC
MvnI 6409 CGCG CG^CG
6431 CGCG
NaeI 5833 GCCGGC GCC^GGC
6679 GCCGGC
NcoI 1427 CCATGG C^CATGG
4136 CCATGG
NdeI 1105 CATATG CA^TATG
1659 CATATG
NgoMIV 5833 GCCGGC G^CCGGC
6679 GCCGGC
NsiI 7088 ATGCAT ATGCA^T
7283 ATGCAT
PflFI 1281 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2746 GACTGAGTC
PshBI 4704 ATTAAT AT^TAAT
5148 ATTAAT
PvuII 301 CAGCTG CAG^CTG
1557 CAGCTG
SphI 1781 GCATGC GCATG^C
4024 GCATGC
TaqI 1700 TCGA T^CGA
4904 TCGA
Tth111I 1281 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2746 GACTGAGTC
TthHB8I 1700 TCGA T^CGA
4904 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspLU11I BspMI
BsrDI BsrFI BsrGI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DraIII DrdI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PstI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SspBI SspI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XbaI XcmI XhoII
XmnI