##################################
# NO CUT ENZYMES (   60 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
ApaI        AscI        Asp718I     AviII       BamHI       
BbrPI       BciVI       BclI        BfrI        BglI        
BsiEI       BsiWI       BspDI       BspHI       BssHII      
BstBI       ClaI        CpoI        EagI        EclXI       
Eco52I      EcoRV       FbaI        FseI        FspI        
HpaI        KpnI        KspI        NheI        NotI        
NruI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PshAI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SapI        SbfI        SfiI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AccIII        268  TCCGGA               T^CCGGA
AocI         3502  CCTAAGG              CC^T[ACGT]AGG
Aor51HI       868  AGCGCT               AGC^GCT
AvrII        2194  CCTAGG               C^CTAGG
BbeI          711  GGCGCC               GGCGC^C
BcgI         5853  CGAGGGCTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI         2194  CCTAGG               C^CTAGG
BlpI         6379  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     6379  GCTGAGC              GC^T[ACGT]AGC
BsaAI        5200  CACGTA               [CT]AC^GT[GA]
BseAI         268  TCCGGA               T^CCGGA
BspEI         268  TCCGGA               T^CCGGA
BsrBI        4462  CCGCTC               CCGCTC(-3/-3)
Bst1107I     7302  GTATAC               GTA^TAC
BstZ17I      7302  GTATAC               GTA^TAC
Bsu36I       3502  CCTAAGG              CC^T[ACGT]AGG
CelII        6379  GCTGAGC              GC^T[ACGT]AGC
Eco47III      868  AGCGCT               AGC^GCT
Eco81I       3502  CCTAAGG              CC^T[ACGT]AGG
KasI          711  GGCGCC               G^GCGCC
MfeI          649  CAATTG               C^AATTG
MluI         6408  ACGCGT               A^CGCGT
MroI          268  TCCGGA               T^CCGGA
MunI          649  CAATTG               C^AATTG
NarI          711  GGCGCC               GG^CGCC
PpuMI        3853  AGGTCCT              [GA]G^G[AT]CC[CT]
SacI         5486  GAGCTC               GAGCT^C
SexAI        2155  ACCAGGT              A^CC[AT]GGT
SfoI          711  GGCGCC               GGC^GCC
SmaI         1807  CCCGGG               CCC^GGG
XmaCI        1807  CCCGGG               C^CCGGG
XmaI         1807  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   40 enzymes) #
###################################

AccII        6409  CGCG                 CG^CG
             6431  CGCG                
AcyI          711  GGCGCC               G[GA]^CG[CT]C
             3544  GACGCC              
AhdI           60  GACTTCCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1122  GACGAGCTGTC         
Alw44I       4382  GTGCAC               G^TGCAC
             4814  GTGCAC              
ApaLI        4382  GTGCAC               G^TGCAC
             4814  GTGCAC              
AseI         4704  ATTAAT               AT^TAAT
             5148  ATTAAT              
AsnI         4704  ATTAAT               AT^TAAT
             5148  ATTAAT              
AspEI          60  GACTTCCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1122  GACGAGCTGTC         
AspI         1281  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2746  GACTGAGTC           
BsaBI        2722  GATTGGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             7309  GATTGTCATC          
BsaHI         711  GGCGCC               G[GA]^CG[CT]C
             3544  GACGCC              
BsaWI          64  TCCGGT               [AT]^CCGG[AT]
              268  TCCGGA              
BsmBI        1300  CGTCTC               CGTCTC(1/5)
             4076  GAGACG              
BssSI        1450  CTCGTG               CACGAG(-5/-1)
             3022  CTCGTG              
BstAPI       5711  GCAAGAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             5772  GCACACCCTGC         
BstEII       1155  GGTTACC              G^GT[ACGT]ACC
             6183  GGTAACC             
BstPI        1155  GGTTACC              G^GT[ACGT]ACC
             6183  GGTAACC             
BstUI        6409  CGCG                 CG^CG
             6431  CGCG                
BstXI        3277  CCATGTGTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             6644  CCAATTTACTGG        
DsaI         1427  CCATGG               C^C[GA][CT]GG
             4136  CCATGG              
Eam1105I       60  GACTTCCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1122  GACGAGCTGTC         
EcoNI         219  CCTGTGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3416  CCTAAAACAGG         
EcoO65I      1155  GGTTACC              G^GT[ACGT]ACC
             6183  GGTAACC             
EcoRI        2381  GAATTC               G^AATTC
             5917  GAATTC              
EcoT22I      7088  ATGCAT               ATGCA^T
             7283  ATGCAT              
Hin1I         711  GGCGCC               G[GA]^CG[CT]C
             3544  GACGCC              
MamI         2722  GATTGGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             7309  GATTGTCATC          
MvnI         6409  CGCG                 CG^CG
             6431  CGCG                
NaeI         5833  GCCGGC               GCC^GGC
             6679  GCCGGC              
NcoI         1427  CCATGG               C^CATGG
             4136  CCATGG              
NdeI         1105  CATATG               CA^TATG
             1659  CATATG              
NgoMIV       5833  GCCGGC               G^CCGGC
             6679  GCCGGC              
NsiI         7088  ATGCAT               ATGCA^T
             7283  ATGCAT              
PflFI        1281  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2746  GACTGAGTC           
PshBI        4704  ATTAAT               AT^TAAT
             5148  ATTAAT              
PvuII         301  CAGCTG               CAG^CTG
             1557  CAGCTG              
SphI         1781  GCATGC               GCATG^C
             4024  GCATGC              
TaqI         1700  TCGA                 T^CGA
             4904  TCGA                
Tth111I      1281  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2746  GACTGAGTC           
TthHB8I      1700  TCGA                 T^CGA
             4904  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (  126 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApoI        Asp700I     
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvCI       BbvI        BcnI        
BfaI        BglII       BmyI        BpmI        BpuAI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspLU11I    BspMI       
BsrDI       BsrFI       BsrGI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraI        
DraII       DraIII      DrdI        EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HincII      HindII      HindIII     
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PstI        RsaI        Sau3AI      
Sau96I      ScaI        ScrFI       SfaNI       SfcI        
SmlI        SspBI       SspI        StuI        StyI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Van91I      XbaI        XcmI        XhoII       
XmnI