##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AclI AflII AflIII AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI AseI AsnI AspEI AspI
AvaI AviII BcgI BfrI BsaBI
BsiWI BsoBI BspDI BspHI BspLU11I
BssHII BssSI BstBI Bsu36I ClaI
CpoI DrdI Eam1105I Eco81I EcoRI
FseI FspI HindIII MamI MluI
NaeI NcoI NgoMIV NheI NruI
NspV PacI PaeR7I PflFI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI SexAI SfiI
SfuI SgrAI SmaI SnaBI SphI
StuI SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 2092 GTATAC GT^[AC][GT]AC
AccIII 2496 TCCGGA T^CCGGA
Aor51HI 543 AGCGCT AGC^GCT
AvrII 4321 CCTAGG C^CTAGG
BamHI 260 GGATCC G^GATCC
BbeI 345 GGCGCC GGCGC^C
BbrPI 194 CACGTG CAC^GTG
BclI 1120 TGATCA T^GATCA
BlnI 4321 CCTAGG C^CTAGG
BsaAI 194 CACGTG [CT]AC^GT[GA]
BsaI 1014 GAGACC GGTCTC(1/5)
BsaWI 2496 TCCGGA [AT]^CCGG[AT]
BseAI 2496 TCCGGA T^CCGGA
BspEI 2496 TCCGGA T^CCGGA
BsrBI 51 GAGCGG CCGCTC(-3/-3)
Bst1107I 2092 GTATAC GTA^TAC
BstEII 1194 GGTCACC G^GT[ACGT]ACC
BstPI 1194 GGTCACC G^GT[ACGT]ACC
BstXI 2621 CCAGCCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2092 GTATAC GTA^TAC
DraI 1746 TTTAAA TTT^AAA
DraIII 4552 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 517 CCGCGG C^C[GA][CT]GG
Eco47III 543 AGCGCT AGC^GCT
EcoNI 752 CCTTTCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1194 GGTCACC G^GT[ACGT]ACC
EcoRV 3971 GATATC GAT^ATC
EcoT22I 4717 ATGCAT ATGCA^T
FbaI 1120 TGATCA T^GATCA
KasI 345 GGCGCC G^GCGCC
KspI 517 CCGCGG CCGC^GG
MroI 2496 TCCGGA T^CCGGA
NarI 345 GGCGCC GG^CGCC
NsiI 4717 ATGCAT ATGCA^T
NspI 4733 GCATGT [GA]CATG^[CT]
PflMI 2042 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 194 CACGTG CAC^GTG
PmlI 194 CACGTG CAC^GTG
PshAI 4211 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 517 CCGCGG CCGC^GG
SbfI 2604 CCTGCAGG CCTGCA^GG
SfoI 345 GGCGCC GGC^GCC
Sse8387I 2604 CCTGCAGG CCTGCA^GG
Van91I 2042 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1266 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Acc65I 479 GGTACC G^GTACC
624 GGTACC
Asp718I 479 GGTACC G^GTACC
624 GGTACC
BbsI 3572 GAAGAC GAAGAC(2/6)
2173 GTCTTC
BciVI 2218 GTATCC GTATCC(6/5)
2563 GTATCC
BglI 56 GCCGGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
79 GCCGACGCGGC
BlpI 762 GCTCAGC GC^T[ACGT]AGC
2550 GCTCAGC
Bpu1102I 762 GCTCAGC GC^T[ACGT]AGC
2550 GCTCAGC
BpuAI 3572 GAAGAC GAAGAC(2/6)
2173 GTCTTC
BsmI 1130 GAATGC GAATGC(1/-1)
3019 GAATGC
BsrFI 342 ACCGGC [GA]^CCGG[CT]
627 ACCGGC
CelII 762 GCTCAGC GC^T[ACGT]AGC
2550 GCTCAGC
Cfr10I 342 ACCGGC [GA]^CCGG[CT]
627 ACCGGC
EarI 938 CTCTTC CTCTTC(1/4)
1011 GAAGAG
KpnI 479 GGTACC GGTAC^C
624 GGTACC
Ksp632I 938 CTCTTC CTCTTC(1/4)
1011 GAAGAG
MslI 3871 CACTCATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4801 CATATAAATG
NotI 73 GCGGCCGC GC^GGCCGC
519 GCGGCCGC
SacI 3401 GAGCTC GAGCT^C
4105 GAGCTC
ScaI 1380 AGTACT AGT^ACT
4243 AGTACT
SspI 1515 AATATT AAT^ATT
2682 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BglII
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I Bsp1407I
BspMI BsrDI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EagI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MfeI MflI MluNI MnlI MscI
MseI MspA1I MspI MunI MvaI
MvnI MwoI NciI NdeI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SpeI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI