##################################
# NO CUT ENZYMES (   64 enzymes) #
##################################

AatII       AccIII      AclI        AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
AspEI       AviII       AvrII       BbrPI       BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaWI       
BseAI       BsiWI       BspDI       BspEI       BssHII      
BssSI       Bst1107I    BstEII      BstPI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraIII      
Eam1105I    Eco47III    Eco81I      EcoO65I     FseI        
FspI        HindIII     HpaI        MfeI        MluI        
MroI        MunI        NruI        PacI        PinAI       
PmaCI       PmeI        PmlI        Psp1406I    PvuI        
RsrII       SalI        SbfI        SexAI       SgrAI       
SnaBI       SphI        Sse8387I    SwaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

Acc65I        570  GGTACC               G^GTACC
AccI         3030  GTAGAC               GT^[AC][GT]AC
AcyI          287  GGCGCC               G[GA]^CG[CT]C
AflIII        756  ACATGT               A^C[GA][CT]GT
ApaI         3258  GGGCCC               GGGCC^C
Asp718I       570  GGTACC               G^GTACC
AspI         1747  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1875  TGGCCA               TGG^CCA
BbeI          287  GGCGCC               GGCGC^C
BbsI         3944  GTCTTC               GAAGAC(2/6)
BbvCI        1537  CCTCAGC              CCTCAGC(-5/-2)
BcgI          898  GCAAGGAATTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        4851  GTATCC               GTATCC(6/5)
BclI         2061  TGATCA               T^GATCA
BpuAI        3944  GTCTTC               GAAGAC(2/6)
BsaBI        2105  GATGATAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         287  GGCGCC               G[GA]^CG[CT]C
BsiEI          96  CGGCCG               CG[GA][CT]^CG
BsmBI         731  GAGACG               CGTCTC(1/5)
BspLU11I      756  ACATGT               A^CATGT
BsrBI          37  GAGCGG               CCGCTC(-3/-3)
BstAPI       1115  GCACCCATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI          361  GACCCGGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI           96  CGGCCG               C^GGCCG
EclXI          96  CGGCCG               C^GGCCG
Eco52I         96  CGGCCG               C^GGCCG
EcoT22I      2930  ATGCAT               ATGCA^T
FbaI         2061  TGATCA               T^GATCA
Hin1I         287  GGCGCC               G[GA]^CG[CT]C
KasI          287  GGCGCC               G^GCGCC
KpnI          570  GGTACC               GGTAC^C
MamI         2105  GATGATAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1875  TGGCCA               TGG^CCA
MscI         1875  TGGCCA               TGG^CCA
NarI          287  GGCGCC               GG^CGCC
NdeI         5165  CATATG               CA^TATG
NheI         4253  GCTAGC               G^CTAGC
NotI           95  GCGGCCGC             GC^GGCCGC
NsiI         2930  ATGCAT               ATGCA^T
PaeR7I        187  CTCGAG               C^TCGAG
PflFI        1747  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SapI         2642  GAAGAGC              GCTCTTC(1/4)
SfiI         3259  GGCCCCCATGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          287  GGCGCC               GGC^GCC
SmaI         2356  CCCGGG               CCC^GGG
SpeI         5404  ACTAGT               A^CTAGT
Tth111I      1747  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI          187  CTCGAG               C^TCGAG
XmaCI        2356  CCCGGG               C^CCGGG
XmaI         2356  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AflII        3861  CTTAAG               C^TTAAG
             4334  CTTAAG              
AseI         4450  ATTAAT               AT^TAAT
             5091  ATTAAT              
AsnI         4450  ATTAAT               AT^TAAT
             5091  ATTAAT              
Asp700I      1528  GAACTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3607  GAAAAAGTTC          
BfrI         3861  CTTAAG               C^TTAAG
             4334  CTTAAG              
BpmI          762  CTCCAG               CTGGAG(16/14)
             2867  CTCCAG              
BsmI         3109  GAATGC               GAATGC(1/-1)
             2932  GCATTC              
Bsp1407I     1275  TGTACA               T^GTACA
             3187  TGTACA              
BspHI        3873  TCATGA               T^CATGA
             4909  TCATGA              
BsrGI        1275  TGTACA               T^GTACA
             3187  TGTACA              
BstBI        2541  TTCGAA               TT^CGAA
             3604  TTCGAA              
DraI         5281  TTTAAA               TTT^AAA
             5466  TTTAAA              
EcoNI         843  CCTTAAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              970  CCTTGACCAGG         
HaeII         203  AGCGCC               [GA]GCGC^[CT]
              287  GGCGCC              
HincII        920  GTTGAC               GT[CT]^[GA]AC
             1744  GTTGAC              
HindII        920  GTTGAC               GT[CT]^[GA]AC
             1744  GTTGAC              
NspV         2541  TTCGAA               TT^CGAA
             3604  TTCGAA              
PshAI         185  GACTCGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1089  GACCAAGGTC          
PshBI        4450  ATTAAT               AT^TAAT
             5091  ATTAAT              
RcaI         3873  TCATGA               T^CATGA
             4909  TCATGA              
ScaI          933  AGTACT               AGT^ACT
             4673  AGTACT              
SfuI         2541  TTCGAA               TT^CGAA
             3604  TTCGAA              
SspBI        1275  TGTACA               T^GTACA
             3187  TGTACA              
SspI          492  AATATT               AAT^ATT
              647  AATATT              
StuI         1695  AGGCCT               AGG^CCT
             4876  AGGCCT              
XbaI         1012  TCTAGA               T^CTAGA
             5079  TCTAGA              
XmnI         1528  GAACTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3607  GAAAAAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  117 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AspHI       AvaI        
AvaII       BamHI       BanI        BanII       BbvI        
BcnI        BfaI        BglI        BmyI        BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrDI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRI       EcoRII      EcoRV       EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     KspI        MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NciI        
NcoI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        SacI        SacII       Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI        XhoII