################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AatII AccIII AclI AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AspEI AviII AvrII BbrPI BglII BlnI BlpI Bpu1102I BsaAI BsaWI BseAI BsiWI BspDI BspEI BssHII BssSI Bst1107I BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraIII Eam1105I Eco47III Eco81I EcoO65I FseI FspI HindIII HpaI MfeI MluI MroI MunI NruI PacI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SalI SbfI SexAI SgrAI SnaBI SphI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Acc65I 570 GGTACC G^GTACC AccI 3030 GTAGAC GT^[AC][GT]AC AcyI 287 GGCGCC G[GA]^CG[CT]C AflIII 756 ACATGT A^C[GA][CT]GT ApaI 3258 GGGCCC GGGCC^C Asp718I 570 GGTACC G^GTACC AspI 1747 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1875 TGGCCA TGG^CCA BbeI 287 GGCGCC GGCGC^C BbsI 3944 GTCTTC GAAGAC(2/6) BbvCI 1537 CCTCAGC CCTCAGC(-5/-2) BcgI 898 GCAAGGAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 4851 GTATCC GTATCC(6/5) BclI 2061 TGATCA T^GATCA BpuAI 3944 GTCTTC GAAGAC(2/6) BsaBI 2105 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 287 GGCGCC G[GA]^CG[CT]C BsiEI 96 CGGCCG CG[GA][CT]^CG BsmBI 731 GAGACG CGTCTC(1/5) BspLU11I 756 ACATGT A^CATGT BsrBI 37 GAGCGG CCGCTC(-3/-3) BstAPI 1115 GCACCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DrdI 361 GACCCGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 96 CGGCCG C^GGCCG EclXI 96 CGGCCG C^GGCCG Eco52I 96 CGGCCG C^GGCCG EcoT22I 2930 ATGCAT ATGCA^T FbaI 2061 TGATCA T^GATCA Hin1I 287 GGCGCC G[GA]^CG[CT]C KasI 287 GGCGCC G^GCGCC KpnI 570 GGTACC GGTAC^C MamI 2105 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1875 TGGCCA TGG^CCA MscI 1875 TGGCCA TGG^CCA NarI 287 GGCGCC GG^CGCC NdeI 5165 CATATG CA^TATG NheI 4253 GCTAGC G^CTAGC NotI 95 GCGGCCGC GC^GGCCGC NsiI 2930 ATGCAT ATGCA^T PaeR7I 187 CTCGAG C^TCGAG PflFI 1747 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC SapI 2642 GAAGAGC GCTCTTC(1/4) SfiI 3259 GGCCCCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 287 GGCGCC GGC^GCC SmaI 2356 CCCGGG CCC^GGG SpeI 5404 ACTAGT A^CTAGT Tth111I 1747 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 187 CTCGAG C^TCGAG XmaCI 2356 CCCGGG C^CCGGG XmaI 2356 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AflII 3861 CTTAAG C^TTAAG 4334 CTTAAG AseI 4450 ATTAAT AT^TAAT 5091 ATTAAT AsnI 4450 ATTAAT AT^TAAT 5091 ATTAAT Asp700I 1528 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3607 GAAAAAGTTC BfrI 3861 CTTAAG C^TTAAG 4334 CTTAAG BpmI 762 CTCCAG CTGGAG(16/14) 2867 CTCCAG BsmI 3109 GAATGC GAATGC(1/-1) 2932 GCATTC Bsp1407I 1275 TGTACA T^GTACA 3187 TGTACA BspHI 3873 TCATGA T^CATGA 4909 TCATGA BsrGI 1275 TGTACA T^GTACA 3187 TGTACA BstBI 2541 TTCGAA TT^CGAA 3604 TTCGAA DraI 5281 TTTAAA TTT^AAA 5466 TTTAAA EcoNI 843 CCTTAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 970 CCTTGACCAGG HaeII 203 AGCGCC [GA]GCGC^[CT] 287 GGCGCC HincII 920 GTTGAC GT[CT]^[GA]AC 1744 GTTGAC HindII 920 GTTGAC GT[CT]^[GA]AC 1744 GTTGAC NspV 2541 TTCGAA TT^CGAA 3604 TTCGAA PshAI 185 GACTCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1089 GACCAAGGTC PshBI 4450 ATTAAT AT^TAAT 5091 ATTAAT RcaI 3873 TCATGA T^CATGA 4909 TCATGA ScaI 933 AGTACT AGT^ACT 4673 AGTACT SfuI 2541 TTCGAA TT^CGAA 3604 TTCGAA SspBI 1275 TGTACA T^GTACA 3187 TGTACA SspI 492 AATATT AAT^ATT 647 AATATT StuI 1695 AGGCCT AGG^CCT 4876 AGGCCT XbaI 1012 TCTAGA T^CTAGA 5079 TCTAGA XmnI 1528 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3607 GAAAAAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BamHI BanI BanII BbvI BcnI BfaI BglI BmyI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRI EcoRII EcoRV EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII