##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII AccII AccIII AcyI AflII
AgeI ApaI AscI AspI AviII
BalI BamHI BbeI BbrPI BciVI
BfrI BglI BlpI Bpu1102I BsaAI
BsaBI BsaHI BseAI BsiWI BspDI
BspEI BspHI BsrBI BssHII BstAPI
BstBI BstEII BstPI BstUI CelII
ClaI CpoI DrdI EcoNI EcoO65I
EcoT22I FseI FspI HgaI Hin1I
HindIII HpaI KasI KspI MamI
MfeI MluI MluNI MroI MscI
MunI MvnI NarI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 1821 GGTACC G^GTACC
AclI 1391 AACGTT AA^CGTT
AflIII 1771 ACATGT A^C[GA][CT]GT
AhdI 1179 GACAGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 139 GTGCAC G^TGCAC
AocI 2912 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1165 AGCGCT AGC^GCT
ApaLI 139 GTGCAC G^TGCAC
Asp700I 921 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1821 GGTACC G^GTACC
AspEI 1179 GACAGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 139 GTGCAC G[AT]GC[AT]^C
AvaI 1189 CCCGAG C^[CT]CG[GA]G
AvrII 3458 CCTAGG C^CTAGG
BanI 1821 GGTACC G^G[CT][GA]CC
BanII 1172 GGGCTC G[GA]GC[CT]^C
BbvCI 1332 CCTCAGC CCTCAGC(-5/-2)
BcgI 131 CGAGATCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 383 AGATCT A^GATCT
BlnI 3458 CCTAGG C^CTAGG
BsaI 1349 GAGACC GGTCTC(1/5)
BsaWI 1 ACCGGA [AT]^CCGG[AT]
BsgI 2970 CTGCAC GTGCAG(16/14)
BsiEI 161 CGGCCG CG[GA][CT]^CG
BsiHKAI 139 GTGCAC G[AT]GC[AT]^C
BsmBI 2866 GAGACG CGTCTC(1/5)
BsoBI 1189 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1604 TGTACA T^GTACA
BspLU11I 1771 ACATGT A^CATGT
BsrFI 1135 GCCGGC [GA]^CCGG[CT]
BsrGI 1604 TGTACA T^GTACA
Bsu36I 2912 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1135 GCCGGC [GA]^CCGG[CT]
DraII 1185 GGGTCCC [GA]G^G[ACGT]CC[CT]
EaeI 161 CGGCCG [CT]^GGCC[GA]
EagI 161 CGGCCG C^GGCCG
Eam1105I 1179 GACAGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 161 CGGCCG C^GGCCG
Eco47III 1165 AGCGCT AGC^GCT
Eco52I 161 CGGCCG C^GGCCG
Eco81I 2912 CCTCAGG CC^T[ACGT]AGG
EcoO109I 1185 GGGTCCC [GA]G^G[ACGT]CC[CT]
FauI 76 CCCGC CCCGC(4/6)
HincII 892 GTCAAC GT[CT]^[GA]AC
HindII 892 GTCAAC GT[CT]^[GA]AC
KpnI 1821 GGTACC GGTAC^C
NaeI 1135 GCCGGC GCC^GGC
NgoMIV 1135 GCCGGC G^CCGGC
PpuMI 1185 GGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 1391 AACGTT AA^CGTT
PvuII 112 CAGCTG CAG^CTG
ScaI 2462 AGTACT AGT^ACT
SspBI 1604 TGTACA T^GTACA
SspI 1661 AATATT AAT^ATT
StuI 8 AGGCCT AGG^CCT
XbaI 2562 TCTAGA T^CTAGA
XmnI 921 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AlwNI 1260 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
1269 CAGAAACTG
AseI 1753 ATTAAT AT^TAAT
2199 ATTAAT
AsnI 1753 ATTAAT AT^TAAT
2199 ATTAAT
BbsI 1494 GAAGAC GAAGAC(2/6)
2575 GAAGAC
BclI 734 TGATCA T^GATCA
1758 TGATCA
BcnI 96 CCGGG CC^[GC]GG
1043 CCGGG
BmyI 139 GTGCAC G[AGT]GC[ACT]^C
1172 GGGCTC
BpuAI 1494 GAAGAC GAAGAC(2/6)
2575 GAAGAC
BseRI 1330 CTCCTC GAGGAG(10/8)
1006 GAGGAG
Bsp1286I 139 GTGCAC G[AGT]GC[ACT]^C
1172 GGGCTC
BspMI 965 GCAGGT ACCTGC(4/8)
1139 GCAGGT
CfoI 1035 GCGC GCG^C
1166 GCGC
DraIII 211 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
1998 CACTTTGTG
DsaI 898 CCATGG C^C[GA][CT]GG
1956 CCATGG
EcoRI 757 GAATTC G^AATTC
1071 GAATTC
EcoRV 2263 GATATC GAT^ATC
3342 GATATC
FbaI 734 TGATCA T^GATCA
1758 TGATCA
HaeII 1034 AGCGCC [GA]GCGC^[CT]
1165 AGCGCT
HhaI 1035 GCGC GCG^C
1166 GCGC
HinP1I 1035 GCGC G^CGC
1166 GCGC
MaeII 846 ACGT A^CGT
1392 ACGT
MspA1I 112 CAGCTG C[AC]G^C[GT]G
1028 CAGCGG
NciI 96 CCGGG CC^[GC]GG
1043 CCGGG
NcoI 898 CCATGG C^CATGG
1956 CCATGG
PflMI 1399 CCATCATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2769 CCACCTCCTGG
PshBI 1753 ATTAAT AT^TAAT
2199 ATTAAT
PstI 1233 CTGCAG CTGCA^G
1266 CTGCAG
SphI 2514 GCATGC GCATG^C
2826 GCATGC
Van91I 1399 CCATCATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2769 CCACCTCCTGG
XcmI 889 CCAGTCAACCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1976 CCACAGGAAGAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AvaII BbvI BfaI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI BsmI BsrDI BsrI BssKI
BssSI Bst1107I BstF5I BstNI BstXI
BstYI BstZ17I Cac8I Cfr13I DdeI
DpnII DraI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NdeI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII