##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI AlwI Aor51HI AscI
AseI AsnI Asp718I AspEI AspI
BamHI BbeI BbvCI BciVI BfrI
BsaBI BsaI BseAI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraI DraIII
Eam1105I Eco47III EcoRV EcoT22I FseI
HincII HindII HindIII HpaI KasI
KpnI MamI MfeI MluI MroI
MunI NarI NdeI NheI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI SbfI ScaI SexAI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 1242 GTCTAC GT^[AC][GT]AC
AfaI 413 GTAC GT^AC
AflIII 684 ACGTGT A^C[GA][CT]GT
Alw44I 1686 GTGCAC G^TGCAC
AocI 1494 CCTGAGG CC^T[ACGT]AGG
ApaI 1823 GGGCCC GGGCC^C
ApaLI 1686 GTGCAC G^TGCAC
Asp700I 243 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 556 TGCGCA TGC^GCA
BcgI 1696 CGAGAGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 469 TGATCA T^GATCA
BglII 289 AGATCT A^GATCT
BlpI 1331 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1331 GCTCAGC GC^T[ACGT]AGC
BsaWI 1841 TCCGGT [AT]^CCGG[AT]
BsmBI 843 CGTCTC CGTCTC(1/5)
BssSI 897 CACGAG CACGAG(-5/-1)
BstAPI 1621 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 386 GGTCACC G^GT[ACGT]ACC
BstPI 386 GGTCACC G^GT[ACGT]ACC
BstXI 430 CCAGTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 289 AGATCT [GA]^GATC[CT]
Bsu36I 1494 CCTGAGG CC^T[ACGT]AGG
CelII 1331 GCTCAGC GC^T[ACGT]AGC
DrdI 366 GACAGGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1134 GAAGAG CTCTTC(1/4)
Eco81I 1494 CCTGAGG CC^T[ACGT]AGG
EcoO65I 386 GGTCACC G^GT[ACGT]ACC
EcoRI 1445 GAATTC G^AATTC
FbaI 469 TGATCA T^GATCA
FspI 556 TGCGCA TGC^GCA
HinfI 859 GAATC G^A[ACGT]TC
Ksp632I 1134 GAAGAG CTCTTC(1/4)
KspI 505 CCGCGG CCGC^GG
MflI 289 AGATCT [GA]^GATC[CT]
MseI 1405 TTAA T^TAA
NaeI 82 GCCGGC GCC^GGC
NcoI 124 CCATGG C^CATGG
NgoMIV 82 GCCGGC G^CCGGC
NotI 1889 GCGGCCGC GC^GGCCGC
NspI 946 ACATGC [GA]CATG^[CT]
RsaI 413 GTAC GT^AC
SacII 505 CCGCGG CCGC^GG
SfiI 1094 GGCCAGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 1117 AGGCCT AGG^CCT
TaqI 591 TCGA T^CGA
TfiI 859 GAATC G^A[AT]TC
Tru9I 1405 TTAA T^TAA
TthHB8I 591 TCGA T^CGA
XhoII 289 AGATCT [GA]^GATC[CT]
XmnI 243 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcsI 201 AAATTT [GA]^AATT[CT]
1445 GAATTC
AcyI 149 GGCGTC G[GA]^CG[CT]C
619 GGCGTC
ApoI 201 AAATTT [GA]^AATT[CT]
1445 GAATTC
AvrII 1371 CCTAGG C^CTAGG
1640 CCTAGG
BbrPI 683 CACGTG CAC^GTG
873 CACGTG
BlnI 1371 CCTAGG C^CTAGG
1640 CCTAGG
BsaAI 683 CACGTG [CT]AC^GT[GA]
873 CACGTG
BsaHI 149 GGCGTC G[GA]^CG[CT]C
619 GGCGTC
BsgI 1628 CTGCAC GTGCAG(16/14)
183 GTGCAG
BsiEI 108 CGGCCG CG[GA][CT]^CG
1890 CGGCCG
BsmI 1736 GAATGC GAATGC(1/-1)
1362 GCATTC
DpnII 290 GATC ^GATC
470 GATC
DsaI 124 CCATGG C^C[GA][CT]GG
505 CCGCGG
EagI 108 CGGCCG C^GGCCG
1890 CGGCCG
EclXI 108 CGGCCG C^GGCCG
1890 CGGCCG
Eco52I 108 CGGCCG C^GGCCG
1890 CGGCCG
EcoNI 1130 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1640 CCTAGGCCAGG
HaeII 750 AGCGCC [GA]GCGC^[CT]
1780 GGCGCT
Hin1I 149 GGCGTC G[GA]^CG[CT]C
619 GGCGTC
MaeII 684 ACGT A^CGT
874 ACGT
MboI 290 GATC ^GATC
470 GATC
MslI 869 CACTCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1001 CACCTTCGTG
NdeII 290 GATC ^GATC
470 GATC
PflMI 271 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
944 CCACATGCTGG
PmaCI 683 CACGTG CAC^GTG
873 CACGTG
PmlI 683 CACGTG CAC^GTG
873 CACGTG
PvuII 966 CAGCTG CAG^CTG
1625 CAGCTG
SacI 795 GAGCTC GAGCT^C
1230 GAGCTC
Sau3AI 290 GATC ^GATC
470 GATC
SmaI 175 CCCGGG CCC^GGG
256 CCCGGG
Van91I 271 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
944 CCACATGCTGG
XcmI 328 CCAGTGCCCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1069 CCACTATGCCTGTGG
XmaCI 175 CCCGGG C^CCGGG
256 CCCGGG
XmaI 175 CCCGGG C^CCGGG
256 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AluI AlwNI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DraII
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HpaII HphI ItaI
MaeI MaeIII MboII MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV PpuMI
PstI Sau96I ScrFI SfaNI SfcI
SmlI StyI TseI Tsp45I Tsp509I
TspRI