##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AccIII AclI AgeI Aor51HI AscI
AviII AvrII BalI BamHI BbrPI
BlnI BsaAI BsaWI BseAI BsiWI
BspDI BspEI BsrBI BsrFI BssHII
Bst1107I BstBI BstZ17I Cfr10I ClaI
CpoI Eco47III FauI FseI FspI
KspI MfeI MluI MluNI MroI
MscI MunI NaeI NcoI NgoMIV
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SfuI SgrAI SnaBI Sse8387I
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 130 GACGTC GACGT^C
Acc65I 360 GGTACC G^GTACC
AccII 7876 CGCG CG^CG
AhdI 3281 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1813 GTGCAC G^TGCAC
ApaI 1374 GGGCCC GGGCC^C
ApaLI 1813 GTGCAC G^TGCAC
AseI 5170 ATTAAT AT^TAAT
AsnI 5170 ATTAAT AT^TAAT
Asp718I 360 GGTACC G^GTACC
AspEI 3281 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 7792 GGCGCC GGCGC^C
BcgI 6639 CGAAAATTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 4195 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 7640 CGGCCG CG[GA][CT]^CG
BssSI 7702 CACGAG CACGAG(-5/-1)
BstAPI 2314 GCACTAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 5678 GGTCACC G^GT[ACGT]ACC
BstPI 5678 GGTCACC G^GT[ACGT]ACC
BstUI 7876 CGCG CG^CG
DraIII 2615 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 3055 CCACGG C^C[GA][CT]GG
EaeI 7640 CGGCCG [CT]^GGCC[GA]
EagI 7640 CGGCCG C^GGCCG
Eam1105I 3281 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 7640 CGGCCG C^GGCCG
Eco52I 7640 CGGCCG C^GGCCG
EcoNI 1417 CCTCGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 5678 GGTCACC G^GT[ACGT]ACC
EcoRI 4961 GAATTC G^AATTC
EcoRV 5822 GATATC GAT^ATC
EcoT22I 35 ATGCAT ATGCA^T
HaeII 7792 GGCGCC [GA]GCGC^[CT]
HgaI 6685 GCGTC GACGC(5/10)
HpaI 1794 GTTAAC GTT^AAC
KasI 7792 GGCGCC G^GCGCC
KpnI 360 GGTACC GGTAC^C
MamI 4195 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 7876 CGCG CG^CG
NarI 7792 GGCGCC GG^CGCC
NheI 1243 GCTAGC G^CTAGC
NsiI 35 ATGCAT ATGCA^T
PshBI 5170 ATTAAT AT^TAAT
SapI 5849 GAAGAGC GCTCTTC(1/4)
ScaI 3134 AGTACT AGT^ACT
SexAI 6455 ACCTGGT A^CC[AT]GGT
SfoI 7792 GGCGCC GGC^GCC
SwaI 693 ATTTAAAT ATTT^AAAT
XbaI 3727 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 130 GACGTC G[GA]^CG[CT]C
7792 GGCGCC
AlwNI 3067 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
3590 CAGTAGCTG
AocI 979 CCTAAGG CC^T[ACGT]AGG
2663 CCTCAGG
AspI 72 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
7911 GACTCCGTC
BglI 969 GCCCCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1644 GCCAGCCAGGC
BsaHI 130 GACGTC G[GA]^CG[CT]C
7792 GGCGCC
BsaI 7719 GAGACC GGTCTC(1/5)
3288 GGTCTC
Bsp1407I 4103 TGTACA T^GTACA
5997 TGTACA
BspHI 1454 TCATGA T^CATGA
2744 TCATGA
BspLU11I 5614 ACATGT A^CATGT
7185 ACATGT
BsrGI 4103 TGTACA T^GTACA
5997 TGTACA
Bsu36I 979 CCTAAGG CC^T[ACGT]AGG
2663 CCTCAGG
CfoI 7793 GCGC GCG^C
7877 GCGC
Eco81I 979 CCTAAGG CC^T[ACGT]AGG
2663 CCTCAGG
HhaI 7793 GCGC GCG^C
7877 GCGC
Hin1I 130 GACGTC G[GA]^CG[CT]C
7792 GGCGCC
HinP1I 7793 GCGC G^CGC
7877 GCGC
PflFI 72 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
7911 GACTCCGTC
PflMI 2016 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5903 CCACTCCTTGG
PpuMI 2011 GGGTCCC [GA]G^G[AT]CC[CT]
5534 AGGACCC
PshAI 1710 GACCCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5471 GACTACTGTC
RcaI 1454 TCATGA T^CATGA
2744 TCATGA
SacI 1215 GAGCTC GAGCT^C
2957 GAGCTC
SmaI 3378 CCCGGG CCC^GGG
7844 CCCGGG
SphI 1400 GCATGC GCATG^C
2964 GCATGC
SspBI 4103 TGTACA T^GTACA
5997 TGTACA
SspI 481 AATATT AAT^ATT
4460 AATATT
Tth111I 72 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
7911 GACTCCGTC
Van91I 2016 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5903 CCACTCCTTGG
XmaCI 3378 CCCGGG C^CCGGG
7844 CCCGGG
XmaI 3378 CCCGGG C^CCGGG
7844 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI AlwI ApoI Asp700I
AspHI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BciVI BclI
BcnI BfaI BfrI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CelII Cfr13I DdeI DpnII DraI
DraII DrdI EarI EcoO109I EcoRII
EcoT14I FbaI Fnu4HI FokI HaeIII
HapII HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SpeI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI