##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AocI AscI AseI AsnI Asp718I
BamHI BbrPI BsaAI BsaBI BseAI
BsiWI BsmBI BspDI BspEI BspLU11I
BssHII Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI Eco81I EcoRV FseI
KpnI KspI MamI MfeI MluI
MroI MunI NheI NruI NspV
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII ScaI SfuI
SnaBI SpeI SwaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AflII 1890 CTTAAG C^TTAAG
AgeI 231 ACCGGT A^CCGGT
Alw44I 7154 GTGCAC G^TGCAC
ApaLI 7154 GTGCAC G^TGCAC
AspI 2447 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 5812 TGCGCA TGC^GCA
AvrII 5936 CCTAGG C^CTAGG
BbeI 2780 GGCGCC GGCGC^C
BfrI 1890 CTTAAG C^TTAAG
BlnI 5936 CCTAGG C^CTAGG
BsaWI 231 ACCGGT [AT]^CCGG[AT]
Bsp1407I 5660 TGTACA T^GTACA
BspHI 502 TCATGA T^CATGA
BsrGI 5660 TGTACA T^GTACA
DraIII 3036 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1011 GACGGCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 5812 TGCGCA TGC^GCA
HindIII 4921 AAGCTT A^AGCTT
KasI 2780 GGCGCC G^GCGCC
NaeI 2777 GCCGGC GCC^GGC
NarI 2780 GGCGCC GG^CGCC
NdeI 5770 CATATG CA^TATG
NgoMIV 2777 GCCGGC G^CCGGC
NotI 2387 GCGGCCGC GC^GGCCGC
PacI 5346 TTAATTAA TTAAT^TAA
PaeR7I 7116 CTCGAG C^TCGAG
PflFI 2447 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 231 ACCGGT A^CCGGT
PshAI 1387 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 502 TCATGA T^CATGA
SapI 638 GCTCTTC GCTCTTC(1/4)
SbfI 2528 CCTGCAGG CCTGCA^GG
SexAI 3037 ACCAGGT A^CC[AT]GGT
SfoI 2780 GGCGCC GGC^GCC
SgrAI 3133 CACCGGCG C[GA]^CCGG[CT]G
Sse8387I 2528 CCTGCAGG CCTGCA^GG
SspBI 5660 TGTACA T^GTACA
SspI 7305 AATATT AAT^ATT
Tth111I 2447 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 7116 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AhdI 4337 GACTGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6318 GACACAAAGTC
AspEI 4337 GACTGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6318 GACACAAAGTC
BcgI 3704 CGACACACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3596 GCATGAAGGTCG
BciVI 4159 GTATCC GTATCC(6/5)
4806 GTATCC
BclI 119 TGATCA T^GATCA
1252 TGATCA
BglII 381 AGATCT A^GATCT
851 AGATCT
BstEII 2198 GGTCACC G^GT[ACGT]ACC
5955 GGTGACC
BstPI 2198 GGTCACC G^GT[ACGT]ACC
5955 GGTGACC
Eam1105I 4337 GACTGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6318 GACACAAAGTC
EcoO65I 2198 GGTCACC G^GT[ACGT]ACC
5955 GGTGACC
EcoT22I 4518 ATGCAT ATGCA^T
4587 ATGCAT
FbaI 119 TGATCA T^GATCA
1252 TGATCA
HpaI 924 GTTAAC GTT^AAC
4673 GTTAAC
NcoI 3988 CCATGG C^CATGG
4552 CCATGG
NsiI 4518 ATGCAT ATGCA^T
4587 ATGCAT
SfiI 2300 GGCCTCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2508 GGCCCACCCGGCC
SmaI 2973 CCCGGG CCC^GGG
4064 CCCGGG
XbaI 5648 TCTAGA T^CTAGA
6380 TCTAGA
XmaCI 2973 CCCGGG C^CCGGG
4064 CCCGGG
XmaI 2973 CCCGGG C^CCGGG
4064 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 145 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI Aor51HI
ApaI ApoI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EagI EarI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI SalI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmnI