##################################
# NO CUT ENZYMES ( 43 enzymes) #
##################################
AatII Acc65I AflII AgeI Alw44I
ApaLI AscI Asp718I AviII AvrII
BalI BbvCI BcgI BfrI BlnI
BsiWI CpoI DrdI EcoRV FseI
FspI HindIII KpnI KspI MluI
MluNI MscI NruI PacI PinAI
PmeI PshAI RsrII SacII SalI
SbfI SexAI SfiI SgrAI SpeI
Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AclI 4151 AACGTT AA^CGTT
AocI 1722 CCTCAGG CC^T[ACGT]AGG
Aor51HI 7580 AGCGCT AGC^GCT
Asp700I 3598 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 6700 GGCGCC GGCGC^C
BlpI 7244 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 7244 GCTGAGC GC^T[ACGT]AGC
BsaI 218 GGTCTC GGTCTC(1/5)
BspLU11I 6424 ACATGT A^CATGT
BsrBI 7393 CCGCTC CCGCTC(-3/-3)
BssSI 2244 CACGAG CACGAG(-5/-1)
Bst1107I 2650 GTATAC GTA^TAC
BstBI 4911 TTCGAA TT^CGAA
BstEII 5603 GGTAACC G^GT[ACGT]ACC
BstPI 5603 GGTAACC G^GT[ACGT]ACC
BstZ17I 2650 GTATAC GTA^TAC
Bsu36I 1722 CCTCAGG CC^T[ACGT]AGG
CelII 7244 GCTGAGC GC^T[ACGT]AGC
DraI 156 TTTAAA TTT^AAA
Eco47III 7580 AGCGCT AGC^GCT
Eco81I 1722 CCTCAGG CC^T[ACGT]AGG
EcoO65I 5603 GGTAACC G^GT[ACGT]ACC
HpaI 6486 GTTAAC GTT^AAC
KasI 6700 GGCGCC G^GCGCC
MfeI 1187 CAATTG C^AATTG
MunI 1187 CAATTG C^AATTG
NarI 6700 GGCGCC GG^CGCC
NheI 2801 GCTAGC G^CTAGC
NotI 6967 GCGGCCGC GC^GGCCGC
NspV 4911 TTCGAA TT^CGAA
PaeR7I 5911 CTCGAG C^TCGAG
Psp1406I 4151 AACGTT AA^CGTT
PvuI 4309 CGATCG CGAT^CG
SacI 609 GAGCTC GAGCT^C
SfoI 6700 GGCGCC GGC^GCC
SfuI 4911 TTCGAA TT^CGAA
SspI 6255 AATATT AAT^ATT
XhoI 5911 CTCGAG C^TCGAG
XmnI 3598 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 2650 GTATAC GT^[AC][GT]AC
6054 GTCTAC
AcyI 6700 GGCGCC G[GA]^CG[CT]C
6765 GGCGTC
ApaI 1379 GGGCCC GGGCC^C
5628 GGGCCC
AseI 3155 ATTAAT AT^TAAT
6314 ATTAAT
AsnI 3155 ATTAAT AT^TAAT
6314 ATTAAT
AspI 2336 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
7001 GACGGTGTC
BsaHI 6700 GGCGCC G[GA]^CG[CT]C
6765 GGCGTC
BsmBI 3811 CGTCTC CGTCTC(1/5)
660 GAGACG
BsmI 849 GAATGC GAATGC(1/-1)
1452 GAATGC
BspDI 2814 ATCGAT AT^CGAT
4311 ATCGAT
ClaI 2814 ATCGAT AT^CGAT
4311 ATCGAT
EagI 6844 CGGCCG C^GGCCG
6968 CGGCCG
EclXI 6844 CGGCCG C^GGCCG
6968 CGGCCG
Eco52I 6844 CGGCCG C^GGCCG
6968 CGGCCG
EcoRI 329 GAATTC G^AATTC
6141 GAATTC
Hin1I 6700 GGCGCC G[GA]^CG[CT]C
6765 GGCGTC
PflFI 2336 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
7001 GACGGTGTC
PshBI 3155 ATTAAT AT^TAAT
6314 ATTAAT
SapI 445 GAAGAGC GCTCTTC(1/4)
491 GCTCTTC
SnaBI 2829 TACGTA TAC^GTA
7281 TACGTA
StuI 6655 AGGCCT AGG^CCT
7638 AGGCCT
Tth111I 2336 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
7001 GACGGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 154 enzymes) #
#########################################
AccII AccIII AciI AcsI AfaI
AflIII AhdI AluI AlwI AlwNI
ApoI AspEI AspHI AvaI AvaII
BamHI BanI BanII BbrPI BbsI
BbvI BciVI BclI BcnI BfaI
BglI BglII BmyI BpmI BpuAI
BsaAI BsaBI BsaJI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspEI BspHI BspMI BsrDI
BsrFI BsrGI BsrI BssHII BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI Eam1105I EarI EcoNI EcoO109I
EcoRII EcoT14I EcoT22I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MroI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeI
NdeII NgoMIV NlaIII NlaIV NsiI
NspI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmaI SmlI SphI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI