##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AclI Aor51HI AscI AviII AvrII
BamHI BbrPI BbvCI BlnI BlpI
Bpu1102I BsaAI BsiEI BsiWI BsrBI
BssHII BstBI BstEII BstPI CelII
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoO65I FseI FspI MluI
NaeI NgoMIV NotI NspV PacI
PflMI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SalI SbfI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI Van91I
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 3678 GACGTC GACGT^C
Acc65I 3496 GGTACC G^GTACC
AccIII 6288 TCCGGA T^CCGGA
AflII 7230 CTTAAG C^TTAAG
AgeI 2908 ACCGGT A^CCGGT
AhdI 5773 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 85 GTGCAC G^TGCAC
ApaLI 85 GTGCAC G^TGCAC
Asp718I 3496 GGTACC G^GTACC
AspEI 5773 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 249 GGCGCC GGCGC^C
BcgI 1738 CGAGGTCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 7230 CTTAAG C^TTAAG
BseAI 6288 TCCGGA T^CCGGA
Bsp1407I 7732 TGTACA T^GTACA
BspDI 4883 ATCGAT AT^CGAT
BspEI 6288 TCCGGA T^CCGGA
BspHI 1185 TCATGA T^CATGA
BsrFI 2908 ACCGGT [GA]^CCGG[CT]
BsrGI 7732 TGTACA T^GTACA
Cfr10I 2908 ACCGGT [GA]^CCGG[CT]
ClaI 4883 ATCGAT AT^CGAT
Eam1105I 5773 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 886 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 249 GGCGCC [GA]GCGC^[CT]
HindIII 6929 AAGCTT A^AGCTT
HpaI 372 GTTAAC GTT^AAC
KasI 249 GGCGCC G^GCGCC
KpnI 3496 GGTACC GGTAC^C
KspI 175 CCGCGG CCGC^GG
MroI 6288 TCCGGA T^CCGGA
NarI 249 GGCGCC GG^CGCC
NheI 64 GCTAGC G^CTAGC
NruI 274 TCGCGA TCG^CGA
PinAI 2908 ACCGGT A^CCGGT
PshAI 6886 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1185 TCATGA T^CATGA
SacII 175 CCGCGG CCGC^GG
SapI 2376 GAAGAGC GCTCTTC(1/4)
SfoI 249 GGCGCC GGC^GCC
SphI 6825 GCATGC GCATG^C
SspBI 7732 TGTACA T^GTACA
StuI 6944 AGGCCT AGG^CCT
XbaI 1987 TCTAGA T^CTAGA
XcmI 2157 CCAGTCTTCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BsaI 235 GAGACC GGTCTC(1/5)
3651 GAGACC
BsmBI 5354 CGTCTC CGTCTC(1/5)
3533 GAGACG
BssSI 5549 CACGAG CACGAG(-5/-1)
572 CTCGTG
Bst1107I 6699 GTATAC GTA^TAC
6854 GTATAC
BstXI 3188 CCACAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6917 CCAAGATCCTGG
BstZ17I 6699 GTATAC GTA^TAC
6854 GTATAC
EcoRI 2758 GAATTC G^AATTC
5460 GAATTC
EcoRV 5181 GATATC GAT^ATC
6168 GATATC
MfeI 1220 CAATTG C^AATTG
6754 CAATTG
MunI 1220 CAATTG C^AATTG
6754 CAATTG
PaeR7I 1546 CTCGAG C^TCGAG
1736 CTCGAG
SexAI 885 ACCTGGT A^CC[AT]GGT
4532 ACCAGGT
SmaI 253 CCCGGG CCC^GGG
1293 CCCGGG
SspI 5536 AATATT AAT^ATT
6793 AATATT
XhoI 1546 CTCGAG C^TCGAG
1736 CTCGAG
XmaCI 253 CCCGGG C^CCGGG
1293 CCCGGG
XmaI 253 CCCGGG C^CCGGG
1293 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApaI ApoI AseI AsnI
Asp700I AspHI AspI AvaI AvaII
BalI BanI BanII BbsI BbvI
BciVI BclI BcnI BfaI BglI
BglII BmyI BpmI BpuAI BsaBI
BsaHI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspLU11I BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I EcoT22I
FauI FbaI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeI NdeII NlaIII NlaIV
NsiI NspI PflFI PleI PpuMI
PshBI PstI PvuII RsaI SacI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XhoII XmnI