##################################
# NO CUT ENZYMES (   73 enzymes) #
##################################

AatII       Acc65I      AccIII      AcyI        AgeI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp718I     AviII       BbeI        BbrPI       BcgI        
BglI        BsaBI       BsaHI       BseAI       BsiWI       
BsmBI       BspEI       BsrFI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     Cfr10I      DrdI        Eco47III    EcoO65I     
EcoRV       FseI        FspI        Hin1I       KasI        
KpnI        MamI        MroI        NaeI        NarI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       PstI        PvuI        
SalI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SphI        Sse8387I    SwaI        
XbaI        XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AclI         6386  AACGTT               AA^CGTT
AhdI         3858  GACATTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       4696  GTGCAC               G^TGCAC
ApaLI        4696  GTGCAC               G^TGCAC
AspEI        3858  GACATTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII        2250  CCTAGG               C^CTAGG
BanI         4420  GGTGCC               G^G[CT][GA]CC
BbvCI         455  GCTGAGG              CCTCAGC(-5/-2)
BclI         6982  TGATCA               T^GATCA
BlnI         2250  CCTAGG               C^CTAGG
BlpI         2442  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2442  GCTGAGC              GC^T[ACGT]AGC
CelII        2442  GCTGAGC              GC^T[ACGT]AGC
CpoI           27  CGGTCCG              CG^G[AT]CCG
DraIII       6869  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          597  CGGCCG               C^GGCCG
Eam1105I     3858  GACATTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         597  CGGCCG               C^GGCCG
Eco52I        597  CGGCCG               C^GGCCG
FbaI         6982  TGATCA               T^GATCA
HaeII         195  GGCGCT               [GA]GCGC^[CT]
HgaI          483  GCGTC                GACGC(5/10)
KspI          366  CCGCGG               CCGC^GG
MluI          481  ACGCGT               A^CGCGT
Psp1406I     6386  AACGTT               AA^CGTT
RsrII          27  CGGTCCG              CG^G[AT]CCG
SacII         366  CCGCGG               CCGC^GG
SmaI          391  CCCGGG               CCC^GGG
XmaCI         391  CCCGGG               C^CCGGG
XmaI          391  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AccI         4133  GTCTAC               GT^[AC][GT]AC
             6164  GTAGAC              
AflII        3381  CTTAAG               C^TTAAG
             7369  CTTAAG              
AspI          116  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2281  GACCCCGTC           
AvaI          391  CCCGGG               C^[CT]CG[GA]G
             2962  CTCGGG              
BbsI         2606  GAAGAC               GAAGAC(2/6)
             2287  GTCTTC              
BfrI         3381  CTTAAG               C^TTAAG
             7369  CTTAAG              
BglII        1637  AGATCT               A^GATCT
             3342  AGATCT              
BpuAI        2606  GAAGAC               GAAGAC(2/6)
             2287  GTCTTC              
BsaAI        4563  TACGTA               [CT]AC^GT[GA]
             7113  TACGTA              
BsaI          541  GGTCTC               GGTCTC(1/5)
              823  GGTCTC              
BsiEI         214  CGACCG               CG[GA][CT]^CG
              597  CGGCCG              
BsoBI         391  CCCGGG               C^[CT]CG[GA]G
             2962  CTCGGG              
BspDI        1666  ATCGAT               AT^CGAT
             4771  ATCGAT              
BspHI        1672  TCATGA               T^CATGA
             3251  TCATGA              
BspMI        1382  GCAGGT               ACCTGC(4/8)
             2681  GCAGGT              
BsrBI         489  GAGCGG               CCGCTC(-3/-3)
              612  GAGCGG              
CfoI          196  GCGC                 GCG^C
             4380  GCGC                
ClaI         1666  ATCGAT               AT^CGAT
             4771  ATCGAT              
EcoNI         732  CCTCTGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             6946  CCTGGCTAAGG         
EcoRI        4809  GAATTC               G^AATTC
             5622  GAATTC              
HhaI          196  GCGC                 GCG^C
             4380  GCGC                
HinP1I        196  GCGC                 G^CGC
             4380  GCGC                
MfeI          149  CAATTG               C^AATTG
             5377  CAATTG              
MunI          149  CAATTG               C^AATTG
             5377  CAATTG              
NcoI         4893  CCATGG               C^CATGG
             7388  CCATGG              
NdeI         5232  CATATG               CA^TATG
             5509  CATATG              
PflFI         116  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2281  GACCCCGTC           
PflMI        5045  CCAACTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5117  CCAGTTGCTGG         
PpuMI         506  GGGACCC              [GA]G^G[AT]CC[CT]
             2279  AGGACCC             
RcaI         1672  TCATGA               T^CATGA
             3251  TCATGA              
SnaBI        4563  TACGTA               TAC^GTA
             7113  TACGTA              
SpeI         6327  ACTAGT               A^CTAGT
             6780  ACTAGT              
Tth111I       116  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2281  GACCCCGTC           
Van91I       5045  CCAACTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5117  CCAGTTGCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  121 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       AocI        ApoI        
Asp700I     AspHI       AvaII       BalI        BamHI       
BanII       BbvI        BciVI       BcnI        BfaI        
BmyI        BpmI        BsaJI       BsaWI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        Bsp1286I    Bsp1407I    BspLU11I    
BsrDI       BsrGI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstXI       BstYI       Bsu36I      
Cac8I       Cfr13I      DdeI        DpnII       DraI        
DraII       DsaI        EaeI        EarI        Eco81I      
EcoO109I    EcoRII      EcoT14I     EcoT22I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HincII      
HindII      HindIII     HinfI       HpaI        HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NsiI        NspI        
PleI        PvuII       RsaI        SacI        SapI        
Sau3AI      Sau96I      ScaI        ScrFI       SfaNI       
SfcI        SmlI        SspBI       SspI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
XmnI