##################################
# NO CUT ENZYMES ( 36 enzymes) #
##################################
AatII AclI AgeI Alw44I ApaLI
AscI BsiWI BspDI BssHII BssSI
BstBI ClaI CpoI EcoRI FseI
KspI MfeI MluI MunI NheI
NotI NruI NspV PacI PinAI
PmeI Psp1406I PvuI RsrII SacII
SalI SapI SfuI SgrAI SnaBI
SwaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 5253 GGTACC G^GTACC
AccIII 2605 TCCGGA T^CCGGA
AseI 5523 ATTAAT AT^TAAT
AsnI 5523 ATTAAT AT^TAAT
Asp718I 5253 GGTACC G^GTACC
AviII 4359 TGCGCA TGC^GCA
BbeI 7758 GGCGCC GGCGC^C
BcgI 5204 CGAAGGTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 8947 TGATCA T^GATCA
BseAI 2605 TCCGGA T^CCGGA
BsiEI 463 CGGCCG CG[GA][CT]^CG
BspEI 2605 TCCGGA T^CCGGA
Bst1107I 4917 GTATAC GTA^TAC
BstZ17I 4917 GTATAC GTA^TAC
DraI 8155 TTTAAA TTT^AAA
EagI 463 CGGCCG C^GGCCG
EclXI 463 CGGCCG C^GGCCG
Eco52I 463 CGGCCG C^GGCCG
FbaI 8947 TGATCA T^GATCA
FspI 4359 TGCGCA TGC^GCA
HpaI 2200 GTTAAC GTT^AAC
KasI 7758 GGCGCC G^GCGCC
KpnI 5253 GGTACC GGTAC^C
MroI 2605 TCCGGA T^CCGGA
NaeI 7961 GCCGGC GCC^GGC
NarI 7758 GGCGCC GG^CGCC
NdeI 4254 CATATG CA^TATG
NgoMIV 7961 GCCGGC G^CCGGC
PshAI 2517 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5523 ATTAAT AT^TAAT
ScaI 6392 AGTACT AGT^ACT
SfiI 2027 GGCCAGACCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 7758 GGCGCC GGC^GCC
SpeI 8534 ACTAGT A^CTAGT
SphI 7466 GCATGC GCATG^C
SspI 4202 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflII 870 CTTAAG C^TTAAG
6796 CTTAAG
Aor51HI 2300 AGCGCT AGC^GCT
5010 AGCGCT
Asp700I 819 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
7926 GAATCCCTTC
AvrII 2950 CCTAGG C^CTAGG
4973 CCTAGG
BfrI 870 CTTAAG C^TTAAG
6796 CTTAAG
BlnI 2950 CCTAGG C^CTAGG
4973 CCTAGG
BsaBI 7523 GATAGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
9755 GATGCGTATC
BsmBI 1854 CGTCTC CGTCTC(1/5)
9591 CGTCTC
Bsp1407I 890 TGTACA T^GTACA
9280 TGTACA
BspLU11I 887 ACATGT A^CATGT
5484 ACATGT
BsrFI 2033 ACCGGC [GA]^CCGG[CT]
7961 GCCGGC
BsrGI 890 TGTACA T^GTACA
9280 TGTACA
Cfr10I 2033 ACCGGC [GA]^CCGG[CT]
7961 GCCGGC
Eco47III 2300 AGCGCT AGC^GCT
5010 AGCGCT
EcoRV 4809 GATATC GAT^ATC
9266 GATATC
HincII 2200 GTTAAC GT[CT]^[GA]AC
2233 GTCAAC
HindII 2200 GTTAAC GT[CT]^[GA]AC
2233 GTCAAC
MamI 7523 GATAGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
9755 GATGCGTATC
SacI 3902 GAGCTC GAGCT^C
8109 GAGCTC
SbfI 540 CCTGCAGG CCTGCA^GG
3456 CCTGCAGG
SexAI 1781 ACCAGGT A^CC[AT]GGT
5995 ACCTGGT
Sse8387I 540 CCTGCAGG CCTGCA^GG
3456 CCTGCAGG
SspBI 890 TGTACA T^GTACA
9280 TGTACA
XbaI 4589 TCTAGA T^CTAGA
6524 TCTAGA
XmnI 819 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
7926 GAATCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 161 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AhdI AluI AlwI
AlwNI AocI ApaI ApoI AspEI
AspHI AspI AvaI AvaII BalI
BamHI BanI BanII BbrPI BbsI
BbvCI BbvI BciVI BcnI BfaI
BglI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DraIII
DrdI DsaI EaeI Eam1105I EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NsiI
NspI PaeR7I PflFI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoI XhoII XmaCI
XmaI