##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI Aor51HI ApaI AscI Asp718I
AspI AviII BbeI BbrPI BcgI
BciVI BglI BlpI Bpu1102I BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspHI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DrdI EagI EclXI
Eco47III Eco52I EcoO65I FauI FseI
FspI HaeII HhaI HinP1I HpaI
KasI KpnI KspI MfeI MluI
MroI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI StuI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AcyI 245 GACGCC G[GA]^CG[CT]C
AflII 1893 CTTAAG C^TTAAG
Alw44I 3308 GTGCAC G^TGCAC
ApaLI 3308 GTGCAC G^TGCAC
AseI 963 ATTAAT AT^TAAT
AsnI 963 ATTAAT AT^TAAT
BalI 1882 TGGCCA TGG^CCA
BamHI 2572 GGATCC G^GATCC
BanI 2650 GGCACC G^G[CT][GA]CC
BanII 2211 GGGCTC G[GA]GC[CT]^C
BfrI 1893 CTTAAG C^TTAAG
BsaAI 2184 CACGTA [CT]AC^GT[GA]
BsaBI 2298 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 245 GACGCC G[GA]^CG[CT]C
BsaI 1235 GGTCTC GGTCTC(1/5)
BsaWI 788 ACCGGA [AT]^CCGG[AT]
BsmI 2801 GCATTC GAATGC(1/-1)
BstXI 2992 CCAGCAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 2567 CCACGG C^C[GA][CT]GG
EaeI 1882 TGGCCA [CT]^GGCC[GA]
EcoRV 3662 GATATC GAT^ATC
Hin1I 245 GACGCC G[GA]^CG[CT]C
HincII 2021 GTCAAC GT[CT]^[GA]AC
HindII 2021 GTCAAC GT[CT]^[GA]AC
HindIII 3472 AAGCTT A^AGCTT
MamI 2298 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1882 TGGCCA TGG^CCA
MscI 1882 TGGCCA TGG^CCA
NheI 68 GCTAGC G^CTAGC
PshAI 1422 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 963 ATTAAT AT^TAAT
PstI 1704 CTGCAG CTGCA^G
ScaI 3108 AGTACT AGT^ACT
SmaI 2496 CCCGGG CCC^GGG
SphI 4447 GCATGC GCATG^C
SspI 1256 AATATT AAT^ATT
XmaCI 2496 CCCGGG C^CCGGG
XmaI 2496 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AciI 2541 CCGC CCGC(-3/-1)
2514 GCGG
AhdI 371 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
834 GACAATGTGTC
AocI 2576 CCTCAGG CC^T[ACGT]AGG
2642 CCTGAGG
AspEI 371 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
834 GACAATGTGTC
AvaI 2496 CCCGGG C^[CT]CG[GA]G
2654 CCCGAG
AvrII 2240 CCTAGG C^CTAGG
2910 CCTAGG
BclI 1265 TGATCA T^GATCA
3620 TGATCA
BlnI 2240 CCTAGG C^CTAGG
2910 CCTAGG
BsmBI 243 GAGACG CGTCTC(1/5)
2481 GAGACG
BsoBI 2496 CCCGGG C^[CT]CG[GA]G
2654 CCCGAG
BsrDI 1465 CATTGC GCAATG(2/0)
4281 CATTGC
BstAPI 4466 GCATACGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4475 GCACATTATGC
Bsu36I 2576 CCTCAGG CC^T[ACGT]AGG
2642 CCTGAGG
DraII 736 AGGCCCC [GA]G^G[ACGT]CC[CT]
2887 AGGCCCC
Eam1105I 371 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
834 GACAATGTGTC
Eco81I 2576 CCTCAGG CC^T[ACGT]AGG
2642 CCTGAGG
EcoNI 454 CCTTGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3261 CCTTTCCCAGG
EcoO109I 736 AGGCCCC [GA]G^G[ACGT]CC[CT]
2887 AGGCCCC
EcoRI 2267 GAATTC G^AATTC
3162 GAATTC
EcoT22I 1079 ATGCAT ATGCA^T
1760 ATGCAT
FbaI 1265 TGATCA T^GATCA
3620 TGATCA
HgaI 245 GACGC GACGC(5/10)
1017 GCGTC
NsiI 1079 ATGCAT ATGCA^T
1760 ATGCAT
PflMI 1190 CCATCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2683 CCAGCTCCTGG
SapI 1112 GAAGAGC GCTCTTC(1/4)
3007 GCTCTTC
Van91I 1190 CCATCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2683 CCAGCTCCTGG
XbaI 407 TCTAGA T^CTAGA
2306 TCTAGA
XcmI 2335 CCAGACTGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2846 CCAGATCTCCATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BbsI BbvCI BbvI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspLU11I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraIII EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI