##################################
# NO CUT ENZYMES ( 43 enzymes) #
##################################
AatII AclI AflII AgeI ApaI
AscI BamHI BbrPI BfrI BsiEI
BsiWI BspDI BssHII BstBI ClaI
CpoI EagI EclXI Eco52I HpaI
KspI NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SfiI SfuI SgrAI
SnaBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 2410 GGTACC G^GTACC
AocI 7465 CCTAAGG CC^T[ACGT]AGG
Asp718I 2410 GGTACC G^GTACC
AviII 934 TGCGCA TGC^GCA
AvrII 6157 CCTAGG C^CTAGG
BbeI 4674 GGCGCC GGCGC^C
BclI 3343 TGATCA T^GATCA
BglI 3306 GCCACATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 6157 CCTAGG C^CTAGG
BsaAI 9163 CACGTA [CT]AC^GT[GA]
BspHI 3340 TCATGA T^CATGA
Bst1107I 11265 GTATAC GTA^TAC
BstZ17I 11265 GTATAC GTA^TAC
Bsu36I 7465 CCTAAGG CC^T[ACGT]AGG
Eco81I 7465 CCTAAGG CC^T[ACGT]AGG
EcoRV 588 GATATC GAT^ATC
FbaI 3343 TGATCA T^GATCA
FseI 2555 GGCCGGCC GGCCGG^CC
FspI 934 TGCGCA TGC^GCA
KasI 4674 GGCGCC G^GCGCC
KpnI 2410 GGTACC GGTAC^C
MluI 10371 ACGCGT A^CGCGT
NarI 4674 GGCGCC GG^CGCC
RcaI 3340 TCATGA T^CATGA
SapI 2582 GAAGAGC GCTCTTC(1/4)
SbfI 1451 CCTGCAGG CCTGCA^GG
SexAI 6118 ACCAGGT A^CC[AT]GGT
SfoI 4674 GGCGCC GGC^GCC
SmaI 5770 CCCGGG CCC^GGG
SpeI 126 ACTAGT A^CTAGT
Sse8387I 1451 CCTGCAGG CCTGCA^GG
XmaCI 5770 CCCGGG C^CCGGG
XmaI 5770 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 10372 CGCG CG^CG
10394 CGCG
Alw44I 8345 GTGCAC G^TGCAC
8777 GTGCAC
Aor51HI 3418 AGCGCT AGC^GCT
4831 AGCGCT
ApaLI 8345 GTGCAC G^TGCAC
8777 GTGCAC
AspI 5244 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
6709 GACTGAGTC
BciVI 1289 GGATAC GTATCC(6/5)
1474 GGATAC
BlpI 1127 GCTGAGC GC^T[ACGT]AGC
10342 GCTGAGC
Bpu1102I 1127 GCTGAGC GC^T[ACGT]AGC
10342 GCTGAGC
BsaBI 6685 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
11272 GATTGTCATC
BsmBI 5263 CGTCTC CGTCTC(1/5)
8039 GAGACG
BsrBI 1064 CCGCTC CCGCTC(-3/-3)
8425 CCGCTC
BssSI 5413 CTCGTG CACGAG(-5/-1)
6985 CTCGTG
BstEII 5118 GGTTACC G^GT[ACGT]ACC
10146 GGTAACC
BstPI 5118 GGTTACC G^GT[ACGT]ACC
10146 GGTAACC
BstUI 10372 CGCG CG^CG
10394 CGCG
CelII 1127 GCTGAGC GC^T[ACGT]AGC
10342 GCTGAGC
Eco47III 3418 AGCGCT AGC^GCT
4831 AGCGCT
EcoO65I 5118 GGTTACC G^GT[ACGT]ACC
10146 GGTAACC
EcoRI 6344 GAATTC G^AATTC
9880 GAATTC
EcoT22I 11051 ATGCAT ATGCA^T
11246 ATGCAT
MamI 6685 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
11272 GATTGTCATC
MfeI 1294 CAATTG C^AATTG
4612 CAATTG
MunI 1294 CAATTG C^AATTG
4612 CAATTG
MvnI 10372 CGCG CG^CG
10394 CGCG
NdeI 5068 CATATG CA^TATG
5622 CATATG
NsiI 11051 ATGCAT ATGCA^T
11246 ATGCAT
PflFI 5244 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
6709 GACTGAGTC
SacI 1640 GAGCTC GAGCT^C
9449 GAGCTC
Tth111I 5244 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
6709 GACTGAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 153 enzymes) #
#########################################
AccI AccIII AciI AcsI AcyI
AfaI AflIII AhdI AluI AlwI
AlwNI ApoI AseI AsnI Asp700I
AspEI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcgI BcnI BfaI BglII BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspEI BspLU11I
BspMI BsrDI BsrFI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DraIII
DrdI DsaI EaeI Eam1105I EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MroI MscI MseI MslI MspA1I
MspI MvaI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PshBI
PstI PvuII RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SphI SspBI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI
XcmI XhoII XmnI