##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII AccII AgeI Alw44I ApaI
ApaLI AscI BsiEI BsiWI BsmBI
BsrBI BsrFI BssHII BstAPI BstBI
BstEII BstPI BstUI Cfr10I CpoI
EagI EclXI Eco52I EcoNI EcoO65I
FseI HpaI KspI MluI MvnI
NaeI NgoMIV NotI NruI NspV
PacI PaeR7I PinAI PmeI PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 723 TCCGGA T^CCGGA
AclI 6519 AACGTT AA^CGTT
Aor51HI 2349 AGCGCT AGC^GCT
AspI 10220 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 9823 TGCGCA TGC^GCA
BamHI 5907 GGATCC G^GATCC
BbeI 8986 GGCGCC GGCGC^C
BcgI 9991 GCAGGGATATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 9728 GCCTTTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 7482 AGATCT A^GATCT
BsaBI 1628 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 723 TCCGGA T^CCGGA
BspDI 2478 ATCGAT AT^CGAT
BspEI 723 TCCGGA T^CCGGA
Bst1107I 9342 GTATAC GTA^TAC
BstZ17I 9342 GTATAC GTA^TAC
ClaI 2478 ATCGAT AT^CGAT
DraIII 766 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 8063 GACTAATTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2349 AGCGCT AGC^GCT
FspI 9823 TGCGCA TGC^GCA
KasI 8986 GGCGCC G^GCGCC
MamI 1628 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 723 TCCGGA T^CCGGA
NarI 8986 GGCGCC GG^CGCC
NheI 5941 GCTAGC G^CTAGC
PflFI 10220 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 6519 AACGTT AA^CGTT
SacI 8102 GAGCTC GAGCT^C
SfoI 8986 GGCGCC GGC^GCC
SmaI 3816 CCCGGG CCC^GGG
SpeI 10525 ACTAGT A^CTAGT
Tth111I 10220 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 3816 CCCGGG C^CCGGG
XmaI 3816 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 5378 GGTACC G^GTACC
5796 GGTACC
AcyI 2328 GGCGTC G[GA]^CG[CT]C
8986 GGCGCC
AflII 5247 CTTAAG C^TTAAG
10925 CTTAAG
AhdI 1109 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4291 GACCTGTGGTC
AseI 2474 ATTAAT AT^TAAT
5271 ATTAAT
AsnI 2474 ATTAAT AT^TAAT
5271 ATTAAT
Asp718I 5378 GGTACC G^GTACC
5796 GGTACC
AspEI 1109 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4291 GACCTGTGGTC
AvrII 4052 CCTAGG C^CTAGG
6061 CCTAGG
BalI 6370 TGGCCA TGG^CCA
9699 TGGCCA
BbrPI 1278 CACGTG CAC^GTG
10063 CACGTG
BfrI 5247 CTTAAG C^TTAAG
10925 CTTAAG
BlnI 4052 CCTAGG C^CTAGG
6061 CCTAGG
BlpI 2827 GCTCAGC GC^T[ACGT]AGC
3039 GCTTAGC
Bpu1102I 2827 GCTCAGC GC^T[ACGT]AGC
3039 GCTTAGC
BsaHI 2328 GGCGTC G[GA]^CG[CT]C
8986 GGCGCC
BsaWI 723 TCCGGA [AT]^CCGG[AT]
5965 ACCGGA
BssSI 1436 CTCGTG CACGAG(-5/-1)
1971 CTCGTG
BstXI 803 CCATCCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2946 CCATGACAATGG
CelII 2827 GCTCAGC GC^T[ACGT]AGC
3039 GCTTAGC
DsaI 1456 CCATGG C^C[GA][CT]GG
7043 CCATGG
Eam1105I 1109 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4291 GACCTGTGGTC
FauI 8151 GCGGG CCCGC(4/6)
10197 GCGGG
HaeII 2349 AGCGCT [GA]GCGC^[CT]
8986 GGCGCC
HgaI 5594 GACGC GACGC(5/10)
2329 GCGTC
Hin1I 2328 GGCGTC G[GA]^CG[CT]C
8986 GGCGCC
KpnI 5378 GGTACC GGTAC^C
5796 GGTACC
MluNI 6370 TGGCCA TGG^CCA
9699 TGGCCA
MscI 6370 TGGCCA TGG^CCA
9699 TGGCCA
MspA1I 1296 CAGCTG C[AC]G^C[GT]G
3254 CAGCTG
NcoI 1456 CCATGG C^CATGG
7043 CCATGG
PmaCI 1278 CACGTG CAC^GTG
10063 CACGTG
PmlI 1278 CACGTG CAC^GTG
10063 CACGTG
PpuMI 3598 GGGACCT [GA]G^G[AT]CC[CT]
6614 AGGACCC
PshBI 2474 ATTAAT AT^TAAT
5271 ATTAAT
PvuII 1296 CAGCTG CAG^CTG
3254 CAGCTG
SphI 9263 GCATGC GCATG^C
9769 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaI AvaII BanI
BanII BbsI BbvCI BbvI BciVI
BclI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspHI BspLU11I BspMI BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EaeI EarI
Eco81I EcoO109I EcoRI EcoRII EcoRV
EcoT14I EcoT22I FbaI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MflI MnlI
MseI MslI MspI MunI MvaI
MwoI NciI NdeI NdeII NlaIII
NlaIV NsiI NspI PflMI PleI
PshAI PstI RcaI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI
XcmI XhoII XmnI