##################################
# NO CUT ENZYMES (   36 enzymes) #
##################################

AclI        AgeI        AscI        AseI        AsnI        
BbeI        BsiWI       Bst1107I    BstBI       BstZ17I     
CpoI        EcoRI       EcoT22I     FseI        KasI        
KspI        NarI        NotI        NruI        NsiI        
NspV        PacI        PinAI       PmeI        PshBI       
Psp1406I    PvuI        RsrII       SacII       SfiI        
SfoI        SfuI        SgrAI       SnaBI       SwaI        
XbaI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AatII        6010  GACGTC               GACGT^C
Acc65I         42  GGTACC               G^GTACC
AccIII       7640  TCCGGA               T^CCGGA
AflII        8308  CTTAAG               C^TTAAG
Asp700I      5892  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I        42  GGTACC               G^GTACC
AviII        5969  TGCGCA               TGC^GCA
AvrII        8262  CCTAGG               C^CTAGG
BcgI         1383  CGACGCTGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1991  TGATCA               T^GATCA
BfrI         8308  CTTAAG               C^TTAAG
BglII        2300  AGATCT               A^GATCT
BlnI         8262  CCTAGG               C^CTAGG
BsaBI       10157  GATACTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        7640  TCCGGA               T^CCGGA
BspDI        2071  ATCGAT               AT^CGAT
BspEI        7640  TCCGGA               T^CCGGA
BssHII       3131  GCGCGC               G^CGCGC
ClaI         2071  ATCGAT               AT^CGAT
FbaI         1991  TGATCA               T^GATCA
FspI         5969  TGCGCA               TGC^GCA
HindIII      7654  AAGCTT               A^AGCTT
HpaI         5113  GTTAAC               GTT^AAC
KpnI           42  GGTACC               GGTAC^C
MamI        10157  GATACTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         7584  CAATTG               C^AATTG
MluI         4574  ACGCGT               A^CGCGT
MroI         7640  TCCGGA               T^CCGGA
MunI         7584  CAATTG               C^AATTG
NdeI         2465  CATATG               CA^TATG
SalI          434  GTCGAC               G^TCGAC
SpeI        10251  ACTAGT               A^CTAGT
SspI          760  AATATT               AAT^ATT
XmnI         5892  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccI          434  GTCGAC               GT^[AC][GT]AC
             2737  GTAGAC              
AhdI         2122  GACCAAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             8681  GACGAAACGTC         
Alw44I       1093  GTGCAC               G^TGCAC
             6097  GTGCAC              
Aor51HI       998  AGCGCT               AGC^GCT
             3209  AGCGCT              
ApaLI        1093  GTGCAC               G^TGCAC
             6097  GTGCAC              
AspEI        2122  GACCAAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             8681  GACGAAACGTC         
BamHI        5124  GGATCC               G^GATCC
             7893  GGATCC              
BciVI        1792  GTATCC               GTATCC(6/5)
             6998  GTATCC              
BsiEI        1966  CGGCCG               CG[GA][CT]^CG
             7778  CGGCCG              
BssSI        5723  CACGAG               CACGAG(-5/-1)
             6052  CACGAG              
DraI         8846  TTTAAA               TTT^AAA
            10122  TTTAAA              
DraIII       3112  CACAGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3280  CACGTGGTG           
DrdI         6688  GACAAACCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             7695  GACCACCAAGTC        
EagI         1966  CGGCCG               C^GGCCG
             7778  CGGCCG              
Eam1105I     2122  GACCAAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             8681  GACGAAACGTC         
EclXI        1966  CGGCCG               C^GGCCG
             7778  CGGCCG              
Eco47III      998  AGCGCT               AGC^GCT
             3209  AGCGCT              
Eco52I       1966  CGGCCG               C^GGCCG
             7778  CGGCCG              
EcoRV        1282  GATATC               GAT^ATC
             2956  GATATC              
SacI         1154  GAGCTC               GAGCT^C
             1932  GAGCTC              
SbfI         3405  CCTGCAGG             CCTGCA^GG
             4437  CCTGCAGG            
ScaI         1044  AGTACT               AGT^ACT
             8119  AGTACT              
SphI          587  GCATGC               GCATG^C
             4427  GCATGC              
Sse8387I     3405  CCTGCAGG             CCTGCA^GG
             4437  CCTGCAGG            


#########################################
# 3 OR MORE CUT ENZYMES (  164 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwI        AlwNI       AocI        
ApaI        ApoI        AspHI       AspI        AvaI        
AvaII       BalI        BanI        BanII       BbrPI       
BbsI        BbvCI       BbvI        BcnI        BfaI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaHI       BsaI        BsaJI       
BsaWI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssKI       BstAPI      BstEII      BstF5I      
BstNI       BstPI       BstUI       BstXI       BstYI       
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NciI        NcoI        NdeII       
NgoMIV      NheI        NlaIII      NlaIV       NspI        
PaeR7I      PflFI       PflMI       PleI        PmaCI       
PmlI        PpuMI       PshAI       PstI        PvuII       
RcaI        RsaI        SapI        Sau3AI      Sau96I      
ScrFI       SexAI       SfaNI       SfcI        SmaI        
SmlI        SspBI       StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     Van91I      XcmI        
XhoI        XhoII       XmaCI       XmaI