##################################
# NO CUT ENZYMES (   47 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AgeI        AhdI        ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       BciVI       BsaWI       
BseAI       BsiWI       BspEI       Bst1107I    BstZ17I     
CpoI        Eam1105I    EcoRI       EcoT22I     HpaI        
KpnI        MfeI        MluI        MroI        MunI        
NdeI        NotI        NsiI        PacI        PinAI       
PmeI        PshBI       Psp1406I    PvuI        RsrII       
SalI        SgrAI       SnaBI       SpeI        SphI        
SspI        SwaI        


###################################
# ONE CUT ENZYMES (   22 enzymes) #
###################################

AatII        3956  GACGTC               GACGT^C
BbrPI        8729  CACGTG               CAC^GTG
BcgI          523  GCAAGCTCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1234  TGATCA               T^GATCA
BsaBI        2243  GATCGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI        1650  ATCGAT               AT^CGAT
BspLU11I     6742  ACATGT               A^CATGT
BssHII        114  GCGCGC               G^CGCGC
BstBI        8604  TTCGAA               TT^CGAA
ClaI         1650  ATCGAT               AT^CGAT
DraI         9005  TTTAAA               TTT^AAA
EcoRV        3789  GATATC               GAT^ATC
FbaI         1234  TGATCA               T^GATCA
HincII       3117  GTCAAC               GT[CT]^[GA]AC
HindII       3117  GTCAAC               GT[CT]^[GA]AC
MamI         2243  GATCGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NruI         6758  TCGCGA               TCG^CGA
NspV         8604  TTCGAA               TT^CGAA
PmaCI        8729  CACGTG               CAC^GTG
PmlI         8729  CACGTG               CAC^GTG
ScaI         4342  AGTACT               AGT^ACT
SfuI         8604  TTCGAA               TT^CGAA


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AflII        2520  CTTAAG               C^TTAAG
             8415  CTTAAG              
AflIII       6742  ACATGT               A^C[GA][CT]GT
             7810  ACACGT              
Aor51HI      4109  AGCGCT               AGC^GCT
             8480  AGCGCT              
AviII        1024  TGCGCA               TGC^GCA
             4904  TGCGCA              
BamHI         358  GGATCC               G^GATCC
             8267  GGATCC              
BfrI         2520  CTTAAG               C^TTAAG
             8415  CTTAAG              
BglII         502  AGATCT               A^GATCT
             3643  AGATCT              
BsmI         1586  GAATGC               GAATGC(1/-1)
             4423  GCATTC              
Bsp1407I     9791  TGTACA               T^GTACA
             9827  TGTACA              
BsrGI        9791  TGTACA               T^GTACA
             9827  TGTACA              
BssSI         846  CACGAG               CACGAG(-5/-1)
             4686  CTCGTG              
BstEII       4658  GGTCACC              G^GT[ACGT]ACC
             5741  GGTTACC             
BstPI        4658  GGTCACC              G^GT[ACGT]ACC
             5741  GGTTACC             
EagI          139  CGGCCG               C^GGCCG
             5840  CGGCCG              
EclXI         139  CGGCCG               C^GGCCG
             5840  CGGCCG              
Eco47III     4109  AGCGCT               AGC^GCT
             8480  AGCGCT              
Eco52I        139  CGGCCG               C^GGCCG
             5840  CGGCCG              
EcoO65I      4658  GGTCACC              G^GT[ACGT]ACC
             5741  GGTTACC             
FseI          136  GGCCGGCC             GGCCGG^CC
              320  GGCCGGCC            
FspI         1024  TGCGCA               TGC^GCA
             4904  TGCGCA              
HindIII      1788  AAGCTT               A^AGCTT
             8608  AAGCTT              
KspI          142  CCGCGG               CCGC^GG
             6732  CCGCGG              
PaeR7I       1091  CTCGAG               C^TCGAG
             3943  CTCGAG              
PshAI        3110  GACCATTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             7517  GACGGCTGTC          
SacII         142  CCGCGG               CCGC^GG
             6732  CCGCGG              
SbfI         3173  CCTGCAGG             CCTGCA^GG
             9251  CCTGCAGG            
SexAI        1045  ACCTGGT              A^CC[AT]GGT
             6248  ACCAGGT             
Sse8387I     3173  CCTGCAGG             CCTGCA^GG
             9251  CCTGCAGG            
SspBI        9791  TGTACA               T^GTACA
             9827  TGTACA              
XbaI         1793  TCTAGA               T^CTAGA
             6188  TCTAGA              
XhoI         1091  CTCGAG               C^TCGAG
             3943  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  158 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
Alw44I      AlwI        AlwNI       AocI        ApaI        
ApaLI       Asp700I     AspHI       AspI        AvaI        
AvaII       AvrII       BalI        BanI        BanII       
BbeI        BbsI        BbvCI       BbvI        BcnI        
BfaI        BglI        BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaHI       
BsaI        BsaJI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsoBI       Bsp1286I    BspHI       BspMI       
BsrBI       BsrDI       BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
BstYI       Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DraIII      DrdI        DsaI        EaeI        EarI        
Eco81I      EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        KasI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeII       NgoMIV      NheI        NlaIII      
NlaIV       NspI        PflFI       PflMI       PleI        
PpuMI       PstI        PvuII       RcaI        RsaI        
SacI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SfiI        SfoI        SmaI        
SmlI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Tth111I     TthHB8I     Van91I      XcmI        XhoII       
XmaCI       XmaI        XmnI