##################################
# NO CUT ENZYMES (   58 enzymes) #
##################################

AatII       AclI        AcyI        AgeI        ApaI        
AscI        AvaI        AviII       AvrII       BbeI        
BbrPI       BlnI        BsaHI       BsiEI       BsiWI       
BsmBI       BsoBI       BsrBI       BssHII      BssSI       
CpoI        EagI        EclXI       Eco52I      FseI        
FspI        HgaI        Hin1I       KasI        KspI        
MluI        NarI        NheI        NotI        NruI        
PacI        PaeR7I      PinAI       PmaCI       PmlI        
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SbfI        SfiI        SfoI        SgrAI       
SmaI        SnaBI       SphI        Sse8387I    StuI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

Acc65I       7639  GGTACC               G^GTACC
AccIII       5489  TCCGGA               T^CCGGA
AflII        1900  CTTAAG               C^TTAAG
AhdI         9567  GACTGGTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI     10399  AGCGCT               AGC^GCT
Asp718I      7639  GGTACC               G^GTACC
AspEI        9567  GACTGGTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         6002  GACTAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI         400  GCTGAGG              CCTCAGC(-5/-2)
BcgI         3112  CGAGAGTCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         1900  CTTAAG               C^TTAAG
BglI         7406  GCCAGCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         8557  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     8557  GCTGAGC              GC^T[ACGT]AGC
BseAI        5489  TCCGGA               T^CCGGA
BspDI        5795  ATCGAT               AT^CGAT
BspEI        5489  TCCGGA               T^CCGGA
BsrFI        4346  GCCGGC               [GA]^CCGG[CT]
BstBI        4499  TTCGAA               TT^CGAA
CelII        8557  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       4346  GCCGGC               [GA]^CCGG[CT]
ClaI         5795  ATCGAT               AT^CGAT
Eam1105I     9567  GACTGGTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III    10399  AGCGCT               AGC^GCT
EcoNI        7699  CCTGCCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI         6250  GCGGG                CCCGC(4/6)
HaeII       10399  AGCGCT               [GA]GCGC^[CT]
KpnI         7639  GGTACC               GGTAC^C
MroI         5489  TCCGGA               T^CCGGA
NaeI         4346  GCCGGC               GCC^GGC
NgoMIV       4346  GCCGGC               G^CCGGC
NspV         4499  TTCGAA               TT^CGAA
PflFI        6002  GACTAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmeI        10619  GTTTAAAC             GTTT^AAAC
PshAI         982  GACAACAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI        1260  ACCTGGT              A^CC[AT]GGT
SfuI         4499  TTCGAA               TT^CGAA
SwaI          251  ATTTAAAT             ATTT^AAAT
Tth111I      6002  GACTAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AccII        1966  CGCG                 CG^CG
             5765  CGCG                
AocI         6212  CCTCAGG              CC^T[ACGT]AGG
             9051  CCTGAGG             
BamHI        3544  GGATCC               G^GATCC
             4318  GGATCC              
BclI         1385  TGATCA               T^GATCA
             4044  TGATCA              
BcnI         1269  CCCGG                CC^[GC]GG
             6994  CCCGG               
BsaAI        1038  CACGTA               [CT]AC^GT[GA]
             8001  TACGTG              
BsaI         7331  GAGACC               GGTCTC(1/5)
             1462  GGTCTC              
BsaWI        5489  TCCGGA               [AT]^CCGG[AT]
             7117  ACCGGA              
BstAPI       5665  GCATGGGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             8939  GCAACCTTTGC         
BstUI        1966  CGCG                 CG^CG
             5765  CGCG                
Bsu36I       6212  CCTCAGG              CC^T[ACGT]AGG
             9051  CCTGAGG             
DrdI         2608  GACCCAGGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3654  GACTATTTTGTC        
Eco81I       6212  CCTCAGG              CC^T[ACGT]AGG
             9051  CCTGAGG             
EcoRV        5228  GATATC               GAT^ATC
             5792  GATATC              
FbaI         1385  TGATCA               T^GATCA
             4044  TGATCA              
HindIII      7725  AAGCTT               A^AGCTT
             8959  AAGCTT              
HpaI         8392  GTTAAC               GTT^AAC
            10294  GTTAAC              
MvnI         1966  CGCG                 CG^CG
             5765  CGCG                
NciI         1269  CCCGG                CC^[GC]GG
             6994  CCCGG               
PstI         3920  CTGCAG               CTGCA^G
             5137  CTGCAG              
SmlI         1900  CTTAAG               C^T[CT][GA]AG
             2197  CTCAAG              


#########################################
# 3 OR MORE CUT ENZYMES (  140 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflIII      
AluI        Alw44I      AlwI        AlwNI       ApaLI       
ApoI        AseI        AsnI        Asp700I     AspHI       
AvaII       BalI        BanI        BanII       BbsI        
BbvI        BciVI       BfaI        BglII       BmyI        
BpmI        BpuAI       BsaBI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        Bsp1286I    Bsp1407I    BspHI       
BspLU11I    BspMI       BsrDI       BsrGI       BsrI        
BssKI       Bst1107I    BstEII      BstF5I      BstNI       
BstPI       BstXI       BstYI       BstZ17I     Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DraII       DraIII      DsaI        EaeI        EarI        
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoT14I     
EcoT22I     Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MamI        MboI        MboII       
MfeI        MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MunI        
MvaI        MwoI        NcoI        NdeI        NdeII       
NlaIII      NlaIV       NsiI        NspI        PflMI       
PleI        PpuMI       PshBI       PvuII       RcaI        
RsaI        SapI        Sau3AI      Sau96I      ScaI        
ScrFI       SfaNI       SfcI        SpeI        SspBI       
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XbaI        XcmI        XhoII       XmnI