################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## AatII AclI AcyI AgeI ApaI AscI AvaI AviII AvrII BbeI BbrPI BlnI BsaHI BsiEI BsiWI BsmBI BsoBI BsrBI BssHII BssSI CpoI EagI EclXI Eco52I FseI FspI HgaI Hin1I KasI KspI MluI NarI NheI NotI NruI PacI PaeR7I PinAI PmaCI PmlI Psp1406I PvuI RsrII SacI SacII SalI SbfI SfiI SfoI SgrAI SmaI SnaBI SphI Sse8387I StuI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 7639 GGTACC G^GTACC AccIII 5489 TCCGGA T^CCGGA AflII 1900 CTTAAG C^TTAAG AhdI 9567 GACTGGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 10399 AGCGCT AGC^GCT Asp718I 7639 GGTACC G^GTACC AspEI 9567 GACTGGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 6002 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvCI 400 GCTGAGG CCTCAGC(-5/-2) BcgI 3112 CGAGAGTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 1900 CTTAAG C^TTAAG BglI 7406 GCCAGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 8557 GCTGAGC GC^T[ACGT]AGC Bpu1102I 8557 GCTGAGC GC^T[ACGT]AGC BseAI 5489 TCCGGA T^CCGGA BspDI 5795 ATCGAT AT^CGAT BspEI 5489 TCCGGA T^CCGGA BsrFI 4346 GCCGGC [GA]^CCGG[CT] BstBI 4499 TTCGAA TT^CGAA CelII 8557 GCTGAGC GC^T[ACGT]AGC Cfr10I 4346 GCCGGC [GA]^CCGG[CT] ClaI 5795 ATCGAT AT^CGAT Eam1105I 9567 GACTGGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 10399 AGCGCT AGC^GCT EcoNI 7699 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 6250 GCGGG CCCGC(4/6) HaeII 10399 AGCGCT [GA]GCGC^[CT] KpnI 7639 GGTACC GGTAC^C MroI 5489 TCCGGA T^CCGGA NaeI 4346 GCCGGC GCC^GGC NgoMIV 4346 GCCGGC G^CCGGC NspV 4499 TTCGAA TT^CGAA PflFI 6002 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmeI 10619 GTTTAAAC GTTT^AAAC PshAI 982 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SexAI 1260 ACCTGGT A^CC[AT]GGT SfuI 4499 TTCGAA TT^CGAA SwaI 251 ATTTAAAT ATTT^AAAT Tth111I 6002 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccII 1966 CGCG CG^CG 5765 CGCG AocI 6212 CCTCAGG CC^T[ACGT]AGG 9051 CCTGAGG BamHI 3544 GGATCC G^GATCC 4318 GGATCC BclI 1385 TGATCA T^GATCA 4044 TGATCA BcnI 1269 CCCGG CC^[GC]GG 6994 CCCGG BsaAI 1038 CACGTA [CT]AC^GT[GA] 8001 TACGTG BsaI 7331 GAGACC GGTCTC(1/5) 1462 GGTCTC BsaWI 5489 TCCGGA [AT]^CCGG[AT] 7117 ACCGGA BstAPI 5665 GCATGGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 8939 GCAACCTTTGC BstUI 1966 CGCG CG^CG 5765 CGCG Bsu36I 6212 CCTCAGG CC^T[ACGT]AGG 9051 CCTGAGG DrdI 2608 GACCCAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3654 GACTATTTTGTC Eco81I 6212 CCTCAGG CC^T[ACGT]AGG 9051 CCTGAGG EcoRV 5228 GATATC GAT^ATC 5792 GATATC FbaI 1385 TGATCA T^GATCA 4044 TGATCA HindIII 7725 AAGCTT A^AGCTT 8959 AAGCTT HpaI 8392 GTTAAC GTT^AAC 10294 GTTAAC MvnI 1966 CGCG CG^CG 5765 CGCG NciI 1269 CCCGG CC^[GC]GG 6994 CCCGG PstI 3920 CTGCAG CTGCA^G 5137 CTGCAG SmlI 1900 CTTAAG C^T[CT][GA]AG 2197 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 140 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI Alw44I AlwI AlwNI ApaLI ApoI AseI AsnI Asp700I AspHI AvaII BalI BanI BanII BbsI BbvI BciVI BfaI BglII BmyI BpmI BpuAI BsaBI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspHI BspLU11I BspMI BsrDI BsrGI BsrI BssKI Bst1107I BstEII BstF5I BstNI BstPI BstXI BstYI BstZ17I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI EcoO109I EcoO65I EcoRI EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MfeI MflI MluNI MnlI MscI MseI MslI MspA1I MspI MunI MvaI MwoI NcoI NdeI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PshBI PvuII RcaI RsaI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SpeI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XcmI XhoII XmnI