##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AcyI        
AgeI        AhdI        Aor51HI     ApaI        AscI        
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BamHI       BanI        BbeI        BbrPI       
BcgI        BciVI       BglI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsoBI       
BspDI       BspEI       BsrBI       BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstZ17I     
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
KasI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MunI        NaeI        NarI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PstI        PvuI        
RsrII       SacII       SalI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    Tth111I     XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccII         877  CGCG                 CG^CG
AclI         1177  AACGTT               AA^CGTT
AflII         182  CTTAAG               C^TTAAG
Alw44I       2016  GTGCAC               G^TGCAC
AlwNI         102  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          689  CCTGAGG              CC^T[ACGT]AGG
ApaLI        2016  GTGCAC               G^TGCAC
BalI         1226  TGGCCA               TGG^CCA
BanII         882  GAGCTC               G[GA]GC[CT]^C
BbsI         1643  GAAGAC               GAAGAC(2/6)
BcnI         1648  CCGGG                CC^[GC]GG
BfrI          182  CTTAAG               C^TTAAG
BglII         738  AGATCT               A^GATCT
BpuAI        1643  GAAGAC               GAAGAC(2/6)
BsaI         1020  GGTCTC               GGTCTC(1/5)
BseRI         507  GAGGAG               GAGGAG(10/8)
BsmAI        1021  GTCTC                GTCTC(1/5)
BsmI         2828  GCATTC               GAATGC(1/-1)
Bsp1407I      810  TGTACA               T^GTACA
BsrGI         810  TGTACA               T^GTACA
BstEII       1592  GGTCACC              G^GT[ACGT]ACC
BstPI        1592  GGTCACC              G^GT[ACGT]ACC
BstUI         877  CGCG                 CG^CG
Bsu36I        689  CCTGAGG              CC^T[ACGT]AGG
DraII        2488  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EaeI         1226  TGGCCA               [CT]^GGCC[GA]
Eco81I        689  CCTGAGG              CC^T[ACGT]AGG
EcoO109I     2488  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I      1592  GGTCACC              G^GT[ACGT]ACC
EcoRI        2364  GAATTC               G^AATTC
EcoRV        3916  GATATC               GAT^ATC
FauI          878  GCGGG                CCCGC(4/6)
HapII        1648  CCGG                 C^CGG
HincII       1004  GTTAAC               GT[CT]^[GA]AC
HindII       1004  GTTAAC               GT[CT]^[GA]AC
HpaI         1004  GTTAAC               GTT^AAC
HpaII        1648  CCGG                 C^CGG
MluNI        1226  TGGCCA               TGG^CCA
MscI         1226  TGGCCA               TGG^CCA
MspI         1648  CCGG                 C^CGG
MvnI          877  CGCG                 CG^CG
NciI         1648  CCGGG                CC^[GC]GG
PflMI         207  CCAAGAGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        2488  GGGTCCT              [GA]G^G[AT]CC[CT]
Psp1406I     1177  AACGTT               AA^CGTT
SacI          882  GAGCTC               GAGCT^C
SapI         3487  GAAGAGC              GCTCTTC(1/4)
ScaI         1386  AGTACT               AGT^ACT
SspBI         810  TGTACA               T^GTACA
SwaI         3936  ATTTAAAT             ATTT^AAAT
Van91I        207  CCAAGAGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI           93  CCACCTACACAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AspHI         882  GAGCTC               G[AT]GC[AT]^C
             2016  GTGCAC              
BbvCI        1785  CCTCAGC              CCTCAGC(-5/-2)
             1872  GCTGAGG             
BmyI          882  GAGCTC               G[AGT]GC[ACT]^C
             2016  GTGCAC              
BpmI          628  CTGGAG               CTGGAG(16/14)
             1965  CTGGAG              
BsiHKAI       882  GAGCTC               G[AT]GC[AT]^C
             2016  GTGCAC              
Bsp1286I      882  GAGCTC               G[AGT]GC[ACT]^C
             2016  GTGCAC              
BstXI        1096  CCACCTTTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1155  CCAGCTGCCTGG        
DsaI         1032  CCATGG               C^C[GA][CT]GG
             3048  CCATGG              
EarI          650  GAAGAG               CTCTTC(1/4)
             3487  GAAGAG              
HindIII      1838  AAGCTT               A^AGCTT
             2347  AAGCTT              
Ksp632I       650  GAAGAG               CTCTTC(1/4)
             3487  GAAGAG              
NcoI         1032  CCATGG               C^CATGG
             3048  CCATGG              
SphI         2605  GCATGC               GCATG^C
             3785  GCATGC              
SspI         3327  AATATT               AAT^ATT
             3665  AATATT              
StuI          186  AGGCCT               AGG^CCT
             1308  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (   83 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        ApoI        AseI        AsnI        Asp700I     
AvaII       BbvI        BclI        BfaI        BsaJI       
BsgI        BsiYI       BslI        BsmFI       BspHI       
BspLU11I    BspMI       BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DraI        EcoRII      EcoT14I     
EcoT22I     FbaI        Fnu4HI      FokI        HaeIII      
HinfI       HphI        ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MvaI        MwoI        
NdeI        NdeII       NlaIII      NlaIV       NsiI        
NspI        PleI        PshBI       PvuII       RcaI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       XmnI