##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AclI AflII AscI AseI AsnI
AviII BfrI BsaBI BsiEI BsiWI
BspDI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI EagI EclXI Eco52I
EcoRI EcoT22I FseI FspI HpaI
KspI MamI MfeI MluI MunI
NotI NruI NsiI NspV PacI
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SfuI SgrAI SnaBI
SpeI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 21 enzymes) #
###################################
AgeI 2678 ACCGGT A^CCGGT
AhdI 1467 GACCCGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 4545 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1467 GACCCGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Bsp1407I 4954 TGTACA T^GTACA
BsrGI 4954 TGTACA T^GTACA
DraI 10196 TTTAAA TTT^AAA
Eam1105I 1467 GACCCGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 6073 GATATC GAT^ATC
HindIII 5860 AAGCTT A^AGCTT
NdeI 8730 CATATG CA^TATG
NheI 5023 GCTAGC G^CTAGC
PaeR7I 8994 CTCGAG C^TCGAG
PinAI 2678 ACCGGT A^CCGGT
SfiI 3951 GGCCATCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 6465 CCCGGG CCC^GGG
SspBI 4954 TGTACA T^GTACA
XhoI 8994 CTCGAG C^TCGAG
XmaCI 6465 CCCGGG C^CCGGG
XmaI 6465 CCCGGG C^CCGGG
XmnI 4545 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AatII 4336 GACGTC GACGT^C
7917 GACGTC
AccII 2929 CGCG CG^CG
3280 CGCG
Alw44I 6889 GTGCAC G^TGCAC
9724 GTGCAC
ApaLI 6889 GTGCAC G^TGCAC
9724 GTGCAC
AspI 4180 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5155 GACATTGTC
BamHI 1010 GGATCC G^GATCC
9899 GGATCC
BcgI 6333 CGACCCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
7578 GCACTGCCGTCG
BciVI 9312 GGATAC GTATCC(6/5)
10037 GTATCC
BclI 7110 TGATCA T^GATCA
7775 TGATCA
BspLU11I 4007 ACATGT A^CATGT
9754 ACATGT
BstEII 5457 GGTCACC G^GT[ACGT]ACC
6696 GGTAACC
BstPI 5457 GGTCACC G^GT[ACGT]ACC
6696 GGTAACC
BstUI 2929 CGCG CG^CG
3280 CGCG
DrdI 883 GACACCTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5476 GACACCAGTGTC
EcoO65I 5457 GGTCACC G^GT[ACGT]ACC
6696 GGTAACC
FbaI 7110 TGATCA T^GATCA
7775 TGATCA
MvnI 2929 CGCG CG^CG
3280 CGCG
NaeI 5084 GCCGGC GCC^GGC
6596 GCCGGC
NgoMIV 5084 GCCGGC G^CCGGC
6596 GCCGGC
PflFI 4180 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5155 GACATTGTC
ScaI 9540 AGTACT AGT^ACT
10129 AGTACT
SexAI 246 ACCAGGT A^CC[AT]GGT
7847 ACCTGGT
Tth111I 4180 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5155 GACATTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 165 enzymes) #
#########################################
Acc65I AccI AccIII AciI AcsI
AcyI AfaI AflIII AluI AlwI
AlwNI AocI Aor51HI ApaI ApoI
Asp718I AspHI AvaI AvaII AvrII
BalI BanI BanII BbeI BbrPI
BbsI BbvCI BbvI BcnI BfaI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco47III Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MroI MscI MseI MslI MspA1I
MspI MvaI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
PshAI PstI PvuII RcaI RsaI
SacI SapI Sau3AI Sau96I SbfI
ScrFI SfaNI SfcI SfoI SmlI
SphI Sse8387I StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII