##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AccII AccIII AgeI
AhdI Aor51HI AscI Asp718I AspEI
AviII BbeI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BsrBI BsrFI
BssHII BssSI BstEII BstPI BstUI
Cfr10I ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I FseI
FspI KasI KpnI KspI MluI
MroI MvnI NaeI NarI NgoMIV
NotI NruI PaeR7I PinAI PmeI
PshAI PvuI RsrII SacII SalI
SbfI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AclI 5632 AACGTT AA^CGTT
AcyI 9916 GACGCC G[GA]^CG[CT]C
Alw44I 3225 GTGCAC G^TGCAC
AocI 2863 CCTCAGG CC^T[ACGT]AGG
ApaI 10385 GGGCCC GGGCC^C
ApaLI 3225 GTGCAC G^TGCAC
AvaI 9896 CCCGAG C^[CT]CG[GA]G
BbrPI 8389 CACGTG CAC^GTG
BcgI 4932 CGAGAACTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 7677 GGATAC GTATCC(6/5)
BglI 10045 GCCTTACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 8389 CACGTG [CT]AC^GT[GA]
BsaHI 9916 GACGCC G[GA]^CG[CT]C
BsaI 9086 GGTCTC GGTCTC(1/5)
BsoBI 9896 CCCGAG C^[CT]CG[GA]G
BsrDI 8042 GCAATG GCAATG(2/0)
BstBI 9993 TTCGAA TT^CGAA
Bsu36I 2863 CCTCAGG CC^T[ACGT]AGG
Eco81I 2863 CCTCAGG CC^T[ACGT]AGG
EcoRI 10348 GAATTC G^AATTC
EcoRV 10690 GATATC GAT^ATC
HaeII 8602 GGCGCT [GA]GCGC^[CT]
HgaI 9916 GACGC GACGC(5/10)
Hin1I 9916 GACGCC G[GA]^CG[CT]C
NheI 3023 GCTAGC G^CTAGC
NspV 9993 TTCGAA TT^CGAA
PacI 7064 TTAATTAA TTAAT^TAA
PmaCI 8389 CACGTG CAC^GTG
PmlI 8389 CACGTG CAC^GTG
Psp1406I 5632 AACGTT AA^CGTT
SfuI 9993 TTCGAA TT^CGAA
SphI 9014 GCATGC GCATG^C
SwaI 10917 ATTTAAAT ATTT^AAAT
TaqI 9994 TCGA T^CGA
TthHB8I 9994 TCGA T^CGA
XbaI 5516 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AflII 5017 CTTAAG C^TTAAG
5872 CTTAAG
BamHI 2189 GGATCC G^GATCC
2390 GGATCC
BanI 1907 GGCACC G^G[CT][GA]CC
2820 GGTGCC
BfrI 5017 CTTAAG C^TTAAG
5872 CTTAAG
BlpI 6328 GCTCAGC GC^T[ACGT]AGC
8536 GCTCAGC
Bpu1102I 6328 GCTCAGC GC^T[ACGT]AGC
8536 GCTCAGC
BseRI 1000 CTCCTC GAGGAG(10/8)
1818 CTCCTC
Bsp1407I 3778 TGTACA T^GTACA
6596 TGTACA
BsrGI 3778 TGTACA T^GTACA
6596 TGTACA
CelII 6328 GCTCAGC GC^T[ACGT]AGC
8536 GCTCAGC
DraIII 642 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
2466 CACATCGTG
EcoNI 1806 CCTGTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4772 CCTTGAGAAGG
SacI 1837 GAGCTC GAGCT^C
8072 GAGCTC
SapI 9388 GAAGAGC GCTCTTC(1/4)
2230 GCTCTTC
SexAI 1397 ACCTGGT A^CC[AT]GGT
4452 ACCTGGT
SspBI 3778 TGTACA T^GTACA
6596 TGTACA
StuI 1814 AGGCCT AGG^CCT
7840 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AspHI AspI AvaII
AvrII BalI BanII BbsI BbvCI
BbvI BclI BcnI BfaI BglII
BlnI BmyI BpmI BpuAI BsaBI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI Bsp1286I
BspHI BspLU11I BspMI BsrI BssKI
Bst1107I BstAPI BstF5I BstNI BstXI
BstYI BstZ17I Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DrdI
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I EcoT22I FauI FbaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MfeI MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MunI
MvaI MwoI NciI NcoI NdeI
NdeII NlaIII NlaIV NsiI NspI
PflFI PflMI PleI PpuMI PshBI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
Van91I XcmI XhoII XmnI