##################################
# NO CUT ENZYMES ( 30 enzymes) #
##################################
AclI AgeI Alw44I ApaLI AscI
BsiWI BspDI ClaI CpoI DraIII
DrdI FseI MluI NheI NotI
NruI PacI PinAI PmeI Psp1406I
PvuI RsrII SbfI SmaI SnaBI
SpeI Sse8387I SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 2980 GACGTC GACGT^C
AviII 759 TGCGCA TGC^GCA
BbrPI 4157 CACGTG CAC^GTG
BclI 8176 TGATCA T^GATCA
BsaAI 4157 CACGTG [CT]AC^GT[GA]
BsmI 8339 GCATTC GAATGC(1/-1)
Bsp1407I 2751 TGTACA T^GTACA
BspLU11I 6233 ACATGT A^CATGT
BsrGI 2751 TGTACA T^GTACA
BssHII 355 GCGCGC G^CGCGC
BstAPI 3706 GCACAACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3330 TTCGAA TT^CGAA
EcoRV 9399 GATATC GAT^ATC
FbaI 8176 TGATCA T^GATCA
FspI 759 TGCGCA TGC^GCA
HpaI 6954 GTTAAC GTT^AAC
NaeI 9886 GCCGGC GCC^GGC
NdeI 7540 CATATG CA^TATG
NgoMIV 9886 GCCGGC G^CCGGC
NspV 3330 TTCGAA TT^CGAA
PaeR7I 204 CTCGAG C^TCGAG
PmaCI 4157 CACGTG CAC^GTG
PmlI 4157 CACGTG CAC^GTG
PshAI 1775 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 4259 GTCGAC G^TCGAC
SfiI 6580 GGCCAGTTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 3330 TTCGAA TT^CGAA
SgrAI 4331 CACCGGCG C[GA]^CCGG[CT]G
SphI 2158 GCATGC GCATG^C
SspBI 2751 TGTACA T^GTACA
SspI 2228 AATATT AAT^ATT
XbaI 6986 TCTAGA T^CTAGA
XhoI 204 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 2928 TCCGGA T^CCGGA
4944 TCCGGA
AflIII 6233 ACATGT A^C[GA][CT]GT
6445 ACACGT
AseI 9646 ATTAAT AT^TAAT
10408 ATTAAT
AsnI 9646 ATTAAT AT^TAAT
10408 ATTAAT
Asp700I 6559 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
7858 GAACCCCTTC
BcgI 3523 CGAGGGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4789 CGACATCAATGC
BciVI 7922 GGATAC GTATCC(6/5)
9944 GGATAC
BsaBI 6684 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
9945 GATACCTATC
BsaWI 2928 TCCGGA [AT]^CCGG[AT]
4944 TCCGGA
BseAI 2928 TCCGGA T^CCGGA
4944 TCCGGA
BsiEI 252 CGGCCG CG[GA][CT]^CG
3072 CGGCCG
BspEI 2928 TCCGGA T^CCGGA
4944 TCCGGA
BssSI 2732 CACGAG CACGAG(-5/-1)
6240 CACGAG
Bst1107I 9002 GTATAC GTA^TAC
10004 GTATAC
BstEII 388 GGTGACC G^GT[ACGT]ACC
7913 GGTAACC
BstPI 388 GGTGACC G^GT[ACGT]ACC
7913 GGTAACC
BstZ17I 9002 GTATAC GTA^TAC
10004 GTATAC
EagI 252 CGGCCG C^GGCCG
3072 CGGCCG
EclXI 252 CGGCCG C^GGCCG
3072 CGGCCG
Eco52I 252 CGGCCG C^GGCCG
3072 CGGCCG
EcoO65I 388 GGTGACC G^GT[ACGT]ACC
7913 GGTAACC
EcoT22I 6104 ATGCAT ATGCA^T
7357 ATGCAT
KspI 647 CCGCGG CCGC^GG
7174 CCGCGG
MamI 6684 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
9945 GATACCTATC
MroI 2928 TCCGGA T^CCGGA
4944 TCCGGA
NsiI 6104 ATGCAT ATGCA^T
7357 ATGCAT
PshBI 9646 ATTAAT AT^TAAT
10408 ATTAAT
SacI 316 GAGCTC GAGCT^C
8290 GAGCTC
SacII 647 CCGCGG CCGC^GG
7174 CCGCGG
SexAI 4401 ACCTGGT A^CC[AT]GGT
4949 ACCTGGT
XmnI 6559 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
7858 GAACCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 164 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AcyI AfaI AflII AhdI AluI
AlwI AlwNI AocI Aor51HI ApaI
ApoI Asp718I AspEI AspHI AspI
AvaI AvaII AvrII BalI BamHI
BanI BanII BbeI BbsI BbvCI
BbvI BcnI BfaI BfrI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI Eam1105I
EarI Eco47III Eco81I EcoNI EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
KasI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MfeI MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MunI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI SapI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SfoI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII