##################################
# NO CUT ENZYMES ( 39 enzymes) #
##################################
Acc65I AgeI Aor51HI AseI AsnI
Asp718I BclI BsaBI BsiWI BspDI
BsrDI Bst1107I BstBI BstZ17I ClaI
DrdI Eco47III FbaI HpaI KpnI
MamI MfeI MunI NdeI NotI
NruI NspV PacI PinAI PmeI
PshBI PvuI SbfI ScaI SfuI
SgrAI SnaBI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 369 GACGTC GACGT^C
AccIII 5658 TCCGGA T^CCGGA
AclI 3006 AACGTT AA^CGTT
AflII 4239 CTTAAG C^TTAAG
AscI 2843 GGCGCGCC GG^CGCGCC
BamHI 6308 GGATCC G^GATCC
BciVI 4428 GTATCC GTATCC(6/5)
BfrI 4239 CTTAAG C^TTAAG
BglII 514 AGATCT A^GATCT
BseAI 5658 TCCGGA T^CCGGA
BsmBI 1527 GAGACG CGTCTC(1/5)
BsmI 1561 GCATTC GAATGC(1/-1)
Bsp1407I 6740 TGTACA T^GTACA
BspEI 5658 TCCGGA T^CCGGA
BspHI 3249 TCATGA T^CATGA
BspLU11I 1504 ACATGT A^CATGT
BsrGI 6740 TGTACA T^GTACA
CpoI 5528 CGGACCG CG^G[AT]CCG
EcoNI 1181 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3245 GAATTC G^AATTC
EcoRV 4722 GATATC GAT^ATC
EcoT22I 3169 ATGCAT ATGCA^T
HincII 5308 GTCGAC GT[CT]^[GA]AC
HindII 5308 GTCGAC GT[CT]^[GA]AC
MluI 1875 ACGCGT A^CGCGT
MroI 5658 TCCGGA T^CCGGA
NheI 5160 GCTAGC G^CTAGC
NsiI 3169 ATGCAT ATGCA^T
PaeR7I 3414 CTCGAG C^TCGAG
PflMI 3489 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 3006 AACGTT AA^CGTT
RcaI 3249 TCATGA T^CATGA
RsrII 5528 CGGACCG CG^G[AT]CCG
SacI 798 GAGCTC GAGCT^C
SalI 5308 GTCGAC G^TCGAC
SexAI 2374 ACCTGGT A^CC[AT]GGT
SfiI 2194 GGCCCCTCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 4484 ACTAGT A^CTAGT
SspBI 6740 TGTACA T^GTACA
SspI 2955 AATATT AAT^ATT
Van91I 3489 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3947 TCTAGA T^CTAGA
XhoI 3414 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 2287 GTCTAC GT^[AC][GT]AC
5308 GTCGAC
Alw44I 1974 GTGCAC G^TGCAC
2481 GTGCAC
ApaLI 1974 GTGCAC G^TGCAC
2481 GTGCAC
AvrII 3317 CCTAGG C^CTAGG
6173 CCTAGG
BlnI 3317 CCTAGG C^CTAGG
6173 CCTAGG
BsaWI 253 TCCGGT [AT]^CCGG[AT]
5658 TCCGGA
BsiEI 116 CGGCCG CG[GA][CT]^CG
120 CGGCCG
BspMI 2050 ACCTGC ACCTGC(4/8)
3529 GCAGGT
BssHII 17 GCGCGC G^CGCGC
2844 GCGCGC
BstEII 157 GGTGACC G^GT[ACGT]ACC
2607 GGTGACC
BstPI 157 GGTGACC G^GT[ACGT]ACC
2607 GGTGACC
BstXI 688 CCACTGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1144 CCATGGATCTGG
DraI 2711 TTTAAA TTT^AAA
4802 TTTAAA
EagI 116 CGGCCG C^GGCCG
120 CGGCCG
EclXI 116 CGGCCG C^GGCCG
120 CGGCCG
Eco52I 116 CGGCCG C^GGCCG
120 CGGCCG
EcoO65I 157 GGTGACC G^GT[ACGT]ACC
2607 GGTGACC
FseI 113 GGCCGGCC GGCCGG^CC
117 GGCCGGCC
KspI 2090 CCGCGG CCGC^GG
2322 CCGCGG
PshAI 5199 GACCCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5998 GACCAAGGTC
SacII 2090 CCGCGG CCGC^GG
2322 CCGCGG
SapI 1211 GAAGAGC GCTCTTC(1/4)
1629 GAAGAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 154 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
AocI ApaI ApoI Asp700I AspEI
AspHI AspI AvaI AvaII AviII
BalI BanI BanII BbeI BbrPI
BbsI BbvCI BbvI BcgI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrBI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI Eam1105I EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HindIII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflFI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI SphI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XcmI
XhoII XmaCI XmaI XmnI