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# NO CUT ENZYMES ( 44 enzymes) #
##################################
AccIII AgeI AhdI AscI Asp700I
AspEI AvrII BclI BlnI BseAI
BsiWI BspEI BssHII BstBI CpoI
DrdI EagI Eam1105I EclXI Eco52I
FbaI FseI HpaI KspI MluI
MroI NdeI NheI NotI NruI
NspV PacI PinAI PmeI PvuI
RsrII SacII SalI ScaI SfuI
SgrAI SnaBI SpeI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 2665 GACGTC GACGT^C
Acc65I 3518 GGTACC G^GTACC
AclI 2710 AACGTT AA^CGTT
AflII 7 CTTAAG C^TTAAG
Aor51HI 1255 AGCGCT AGC^GCT
ApaI 1387 GGGCCC GGGCC^C
AseI 5681 ATTAAT AT^TAAT
AsnI 5681 ATTAAT AT^TAAT
Asp718I 3518 GGTACC G^GTACC
AviII 6399 TGCGCA TGC^GCA
BamHI 3727 GGATCC G^GATCC
BbeI 1324 GGCGCC GGCGC^C
BbrPI 6668 CACGTG CAC^GTG
BbvCI 5633 GCTGAGG CCTCAGC(-5/-2)
BfrI 7 CTTAAG C^TTAAG
BglII 1925 AGATCT A^GATCT
BsaAI 6668 CACGTG [CT]AC^GT[GA]
BsaBI 1743 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1712 ACCGGA [AT]^CCGG[AT]
BsiEI 1107 CGACCG CG[GA][CT]^CG
Bsp1407I 5093 TGTACA T^GTACA
BsrGI 5093 TGTACA T^GTACA
Eco47III 1255 AGCGCT AGC^GCT
EcoRV 2110 GATATC GAT^ATC
FspI 6399 TGCGCA TGC^GCA
HincII 2467 GTTGAC GT[CT]^[GA]AC
HindII 2467 GTTGAC GT[CT]^[GA]AC
HindIII 2289 AAGCTT A^AGCTT
KasI 1324 GGCGCC G^GCGCC
KpnI 3518 GGTACC GGTAC^C
MamI 1743 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5525 CAATTG C^AATTG
MunI 5525 CAATTG C^AATTG
NarI 1324 GGCGCC GG^CGCC
PmaCI 6668 CACGTG CAC^GTG
PmlI 6668 CACGTG CAC^GTG
PshAI 3777 GACCTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5681 ATTAAT AT^TAAT
Psp1406I 2710 AACGTT AA^CGTT
SapI 430 GAAGAGC GCTCTTC(1/4)
SbfI 1827 CCTGCAGG CCTGCA^GG
SexAI 652 ACCAGGT A^CC[AT]GGT
SfiI 1557 GGCCCCCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1324 GGCGCC GGC^GCC
SphI 3671 GCATGC GCATG^C
Sse8387I 1827 CCTGCAGG CCTGCA^GG
SspBI 5093 TGTACA T^GTACA
SwaI 6286 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Alw44I 1792 GTGCAC G^TGCAC
3755 GTGCAC
AocI 1662 CCTGAGG CC^T[ACGT]AGG
2948 CCTCAGG
ApaLI 1792 GTGCAC G^TGCAC
3755 GTGCAC
AspI 2296 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4433 GACAGCGTC
BciVI 4907 GGATAC GTATCC(6/5)
5615 GGATAC
BsmI 753 GAATGC GAATGC(1/-1)
3465 GAATGC
BspDI 3280 ATCGAT AT^CGAT
3322 ATCGAT
BspHI 2837 TCATGA T^CATGA
5573 TCATGA
BspLU11I 6058 ACATGT A^CATGT
6162 ACATGT
Bst1107I 6330 GTATAC GTA^TAC
6366 GTATAC
BstAPI 251 GCAGCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
519 GCAGTCTGTGC
BstZ17I 6330 GTATAC GTA^TAC
6366 GTATAC
Bsu36I 1662 CCTGAGG CC^T[ACGT]AGG
2948 CCTCAGG
ClaI 3280 ATCGAT AT^CGAT
3322 ATCGAT
Eco81I 1662 CCTGAGG CC^T[ACGT]AGG
2948 CCTCAGG
EcoT22I 3924 ATGCAT ATGCA^T
5270 ATGCAT
NsiI 3924 ATGCAT ATGCA^T
5270 ATGCAT
PflFI 2296 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4433 GACAGCGTC
RcaI 2837 TCATGA T^CATGA
5573 TCATGA
SacI 139 GAGCTC GAGCT^C
4339 GAGCTC
SmaI 1438 CCCGGG CCC^GGG
1652 CCCGGG
StuI 3260 AGGCCT AGG^CCT
3830 AGGCCT
Tth111I 2296 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4433 GACAGCGTC
XmaCI 1438 CCCGGG C^CCGGG
1652 CCCGGG
XmaI 1438 CCCGGG C^CCGGG
1652 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcgI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BssSI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI EcoNI EcoO109I
EcoO65I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PaeR7I PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XcmI XhoI
XhoII