################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AgeI AhdI AocI Aor51HI AscI Asp700I Asp718I AspEI AviII AvrII BalI BbeI BbrPI BbvCI BcgI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BsiEI BsiWI BspDI BspHI BsrBI BsrFI BssHII Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I FseI FspI HgaI Hin1I HindIII KasI KpnI KspI MamI MfeI MluI MluNI MscI MunI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NspV PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SpeI Sse8387I SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 4814 GTCTAC GT^[AC][GT]AC AccIII 1190 TCCGGA T^CCGGA AflII 6214 CTTAAG C^TTAAG AlwNI 572 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 2032 GGGCCC GGGCC^C AspI 3527 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 968 CCCGGG C^[CT]CG[GA]G BanI 5269 GGTGCC G^G[CT][GA]CC BciVI 3348 GTATCC GTATCC(6/5) BclI 2006 TGATCA T^GATCA BfrI 6214 CTTAAG C^TTAAG BsaAI 3462 TACGTA [CT]AC^GT[GA] BsaI 820 GGTCTC GGTCTC(1/5) BsaWI 1190 TCCGGA [AT]^CCGG[AT] BseAI 1190 TCCGGA T^CCGGA BsgI 1599 GTGCAG GTGCAG(16/14) BsmBI 556 CGTCTC CGTCTC(1/5) BsoBI 968 CCCGGG C^[CT]CG[GA]G Bsp1407I 4264 TGTACA T^GTACA BspEI 1190 TCCGGA T^CCGGA BspMI 4272 ACCTGC ACCTGC(4/8) BsrGI 4264 TGTACA T^GTACA BssSI 2125 CTCGTG CACGAG(-5/-1) BstEII 2169 GGTGACC G^GT[ACGT]ACC BstPI 2169 GGTGACC G^GT[ACGT]ACC EaeI 1343 TGGCCG [CT]^GGCC[GA] EcoO65I 2169 GGTGACC G^GT[ACGT]ACC FauI 1939 CCCGC CCCGC(4/6) FbaI 2006 TGATCA T^GATCA HaeII 593 AGCGCC [GA]GCGC^[CT] MroI 1190 TCCGGA T^CCGGA PacI 2548 TTAATTAA TTAAT^TAA PflFI 3527 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 4662 CCAGCTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 649 GGGTCCC [GA]G^G[AT]CC[CT] SmaI 968 CCCGGG CCC^GGG SnaBI 3462 TACGTA TAC^GTA SspBI 4264 TGTACA T^GTACA StuI 2232 AGGCCT AGG^CCT Tth111I 3527 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 4662 CCAGCTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 3219 TCTAGA T^CTAGA XmaCI 968 CCCGGG C^CCGGG XmaI 968 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AflIII 5135 ACATGT A^C[GA][CT]GT 5197 ACATGT AvaII 650 GGTCC G^G[AT]CC 5387 GGTCC BamHI 2465 GGATCC G^GATCC 3810 GGATCC BanII 965 GAGCCC G[GA]GC[CT]^C 2032 GGGCCC BbsI 3283 GAAGAC GAAGAC(2/6) 1304 GTCTTC BcnI 968 CCCGG CC^[GC]GG 969 CCGGG BpuAI 3283 GAAGAC GAAGAC(2/6) 1304 GTCTTC BsmI 5440 GAATGC GAATGC(1/-1) 1569 GCATTC BspLU11I 5135 ACATGT A^CATGT 5197 ACATGT DraII 649 GGGTCCC [GA]G^G[ACGT]CC[CT] 2031 GGGGCCC DsaI 918 CCGTGG C^C[GA][CT]GG 1346 CCGTGG EcoNI 1554 CCTCCTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2153 CCTGGTGAAGG EcoO109I 649 GGGTCCC [GA]G^G[ACGT]CC[CT] 2031 GGGGCCC EcoRI 2139 GAATTC G^AATTC 4008 GAATTC EcoRV 3236 GATATC GAT^ATC 4408 GATATC HapII 969 CCGG C^CGG 1191 CCGG HpaII 969 CCGG C^CGG 1191 CCGG MspI 969 CCGG C^CGG 1191 CCGG NciI 968 CCCGG CC^[GC]GG 969 CCGGG NdeI 1282 CATATG CA^TATG 1993 CATATG PleI 935 GAGTC GAGTC(4/5) 4613 GAGTC SmlI 3230 CTCAAG C^T[CT][GA]AG 6214 CTTAAG SphI 1617 GCATGC GCATG^C 3567 GCATGC XcmI 2120 CCATTCTCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3836 CCAGCACTGCTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AciI AcsI AfaI AluI Alw44I AlwI ApaLI ApoI AseI AsnI AspHI BbvI BfaI BglII BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI EarI EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HhaI HinP1I HincII HindII HinfI HpaI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NsiI NspI PshBI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII