##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcyI        
AgeI        AhdI        AocI        Aor51HI     AscI        
Asp700I     Asp718I     AspEI       AviII       AvrII       
BalI        BbeI        BbrPI       BbvCI       BcgI        
BglI        BlnI        BlpI        Bpu1102I    BsaBI       
BsaHI       BsiEI       BsiWI       BspDI       BspHI       
BsrBI       BsrFI       BssHII      Bst1107I    BstAPI      
BstBI       BstUI       BstXI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      FseI        FspI        HgaI        
Hin1I       HindIII     KasI        KpnI        KspI        
MamI        MfeI        MluI        MluNI       MscI        
MunI        MvnI        NaeI        NarI        NcoI        
NgoMIV      NheI        NotI        NruI        NspV        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SpeI        Sse8387I    SwaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI         4814  GTCTAC               GT^[AC][GT]AC
AccIII       1190  TCCGGA               T^CCGGA
AflII        6214  CTTAAG               C^TTAAG
AlwNI         572  CAGTAACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         2032  GGGCCC               GGGCC^C
AspI         3527  GACATAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          968  CCCGGG               C^[CT]CG[GA]G
BanI         5269  GGTGCC               G^G[CT][GA]CC
BciVI        3348  GTATCC               GTATCC(6/5)
BclI         2006  TGATCA               T^GATCA
BfrI         6214  CTTAAG               C^TTAAG
BsaAI        3462  TACGTA               [CT]AC^GT[GA]
BsaI          820  GGTCTC               GGTCTC(1/5)
BsaWI        1190  TCCGGA               [AT]^CCGG[AT]
BseAI        1190  TCCGGA               T^CCGGA
BsgI         1599  GTGCAG               GTGCAG(16/14)
BsmBI         556  CGTCTC               CGTCTC(1/5)
BsoBI         968  CCCGGG               C^[CT]CG[GA]G
Bsp1407I     4264  TGTACA               T^GTACA
BspEI        1190  TCCGGA               T^CCGGA
BspMI        4272  ACCTGC               ACCTGC(4/8)
BsrGI        4264  TGTACA               T^GTACA
BssSI        2125  CTCGTG               CACGAG(-5/-1)
BstEII       2169  GGTGACC              G^GT[ACGT]ACC
BstPI        2169  GGTGACC              G^GT[ACGT]ACC
EaeI         1343  TGGCCG               [CT]^GGCC[GA]
EcoO65I      2169  GGTGACC              G^GT[ACGT]ACC
FauI         1939  CCCGC                CCCGC(4/6)
FbaI         2006  TGATCA               T^GATCA
HaeII         593  AGCGCC               [GA]GCGC^[CT]
MroI         1190  TCCGGA               T^CCGGA
PacI         2548  TTAATTAA             TTAAT^TAA
PflFI        3527  GACATAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        4662  CCAGCTATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         649  GGGTCCC              [GA]G^G[AT]CC[CT]
SmaI          968  CCCGGG               CCC^GGG
SnaBI        3462  TACGTA               TAC^GTA
SspBI        4264  TGTACA               T^GTACA
StuI         2232  AGGCCT               AGG^CCT
Tth111I      3527  GACATAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       4662  CCAGCTATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         3219  TCTAGA               T^CTAGA
XmaCI         968  CCCGGG               C^CCGGG
XmaI          968  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AflIII       5135  ACATGT               A^C[GA][CT]GT
             5197  ACATGT              
AvaII         650  GGTCC                G^G[AT]CC
             5387  GGTCC               
BamHI        2465  GGATCC               G^GATCC
             3810  GGATCC              
BanII         965  GAGCCC               G[GA]GC[CT]^C
             2032  GGGCCC              
BbsI         3283  GAAGAC               GAAGAC(2/6)
             1304  GTCTTC              
BcnI          968  CCCGG                CC^[GC]GG
              969  CCGGG               
BpuAI        3283  GAAGAC               GAAGAC(2/6)
             1304  GTCTTC              
BsmI         5440  GAATGC               GAATGC(1/-1)
             1569  GCATTC              
BspLU11I     5135  ACATGT               A^CATGT
             5197  ACATGT              
DraII         649  GGGTCCC              [GA]G^G[ACGT]CC[CT]
             2031  GGGGCCC             
DsaI          918  CCGTGG               C^C[GA][CT]GG
             1346  CCGTGG              
EcoNI        1554  CCTCCTAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2153  CCTGGTGAAGG         
EcoO109I      649  GGGTCCC              [GA]G^G[ACGT]CC[CT]
             2031  GGGGCCC             
EcoRI        2139  GAATTC               G^AATTC
             4008  GAATTC              
EcoRV        3236  GATATC               GAT^ATC
             4408  GATATC              
HapII         969  CCGG                 C^CGG
             1191  CCGG                
HpaII         969  CCGG                 C^CGG
             1191  CCGG                
MspI          969  CCGG                 C^CGG
             1191  CCGG                
NciI          968  CCCGG                CC^[GC]GG
              969  CCGGG               
NdeI         1282  CATATG               CA^TATG
             1993  CATATG              
PleI          935  GAGTC                GAGTC(4/5)
             4613  GAGTC               
SmlI         3230  CTCAAG               C^T[CT][GA]AG
             6214  CTTAAG              
SphI         1617  GCATGC               GCATG^C
             3567  GCATGC              
XcmI         2120  CCATTCTCGTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3836  CCAGCACTGCTGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        Alw44I      
AlwI        ApaLI       ApoI        AseI        AsnI        
AspHI       BbvI        BfaI        BglII       BmyI        
BpmI        BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrDI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        EarI        EcoRII      EcoT14I     EcoT22I     
Fnu4HI      FokI        HaeIII      HhaI        HinP1I      
HincII      HindII      HinfI       HpaI        HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MvaI        MwoI        NdeII       
NlaIII      NlaIV       NsiI        NspI        PshBI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SspI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII