##################################
# NO CUT ENZYMES ( 43 enzymes) #
##################################
AatII AccI AclI AgeI AseI
AsnI BciVI BclI BsiWI BsmI
BspDI BspLU11I Bst1107I BstAPI BstZ17I
ClaI DraI EcoRI EcoRV FbaI
HincII HindII HpaI MfeI MunI
NdeI NheI NotI NruI PacI
PinAI PmeI PshBI Psp1406I PvuI
SacI SalI SexAI SgrAI SpeI
SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 2386 GGTACC G^GTACC
AcsI 6943 GAATTT [GA]^AATT[CT]
AflII 6679 CTTAAG C^TTAAG
AhdI 3149 GACAGTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2871 GTGCAC G^TGCAC
ApaLI 2871 GTGCAC G^TGCAC
ApoI 6943 GAATTT [GA]^AATT[CT]
AscI 2593 GGCGCGCC GG^CGCGCC
Asp718I 2386 GGTACC G^GTACC
AspEI 3149 GACAGTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3241 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 6789 CCTAGG C^CTAGG
BfrI 6679 CTTAAG C^TTAAG
BglII 650 AGATCT A^GATCT
BlnI 6789 CCTAGG C^CTAGG
BlpI 6794 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 6794 GCTTAGC GC^T[ACGT]AGC
BsaBI 2357 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 3158 TCATGA T^CATGA
BsrBI 2301 GAGCGG CCGCTC(-3/-3)
BsrDI 6531 GCAATG GCAATG(2/0)
BssHII 2594 GCGCGC G^CGCGC
BstBI 6603 TTCGAA TT^CGAA
BstEII 1512 GGTCACC G^GT[ACGT]ACC
BstPI 1512 GGTCACC G^GT[ACGT]ACC
CelII 6794 GCTTAGC GC^T[ACGT]AGC
CpoI 6477 CGGACCG CG^G[AT]CCG
DraIII 2681 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 682 GACCCCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3149 GACAGTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1512 GGTCACC G^GT[ACGT]ACC
EcoT22I 3627 ATGCAT ATGCA^T
FseI 3652 GGCCGGCC GGCCGG^CC
KpnI 2386 GGTACC GGTAC^C
MamI 2357 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 6305 ACGCGT A^CGCGT
NcoI 104 CCATGG C^CATGG
NsiI 3627 ATGCAT ATGCA^T
NspV 6603 TTCGAA TT^CGAA
PflFI 3241 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1256 GACTCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3158 TCATGA T^CATGA
RsrII 6477 CGGACCG CG^G[AT]CCG
SapI 524 GCTCTTC GCTCTTC(1/4)
ScaI 1013 AGTACT AGT^ACT
SfiI 2417 GGCCCTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 6603 TTCGAA TT^CGAA
SphI 3625 GCATGC GCATG^C
TfiI 3323 GATTC G^A[AT]TC
Tth111I 3241 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
ApaI 1688 GGGCCC GGGCC^C
2953 GGGCCC
Asp700I 222 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1548 GAACCCCTTC
BamHI 1470 GGATCC G^GATCC
1897 GGATCC
BbrPI 1572 CACGTG CAC^GTG
1891 CACGTG
BglI 2418 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3057 GCCTGGCAGGC
BsiEI 1149 CGGCCG CG[GA][CT]^CG
1217 CGGCCG
BssSI 454 CACGAG CACGAG(-5/-1)
748 CACGAG
EagI 1149 CGGCCG C^GGCCG
1217 CGGCCG
EclXI 1149 CGGCCG C^GGCCG
1217 CGGCCG
Eco52I 1149 CGGCCG C^GGCCG
1217 CGGCCG
KspI 1643 CCGCGG CCGC^GG
2106 CCGCGG
PmaCI 1572 CACGTG CAC^GTG
1891 CACGTG
PmlI 1572 CACGTG CAC^GTG
1891 CACGTG
SacII 1643 CCGCGG CCGC^GG
2106 CCGCGG
SbfI 2464 CCTGCAGG CCTGCA^GG
3563 CCTGCAGG
SnaBI 2898 TACGTA TAC^GTA
6260 TACGTA
Sse8387I 2464 CCTGCAGG CCTGCA^GG
3563 CCTGCAGG
XmnI 222 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1548 GAACCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
Aor51HI AspHI AvaI AvaII AviII
BalI BanI BanII BbeI BbsI
BbvCI BbvI BcgI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BspEI BspMI BsrFI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco47III Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HindIII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PaeR7I PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI SspBI StuI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoI XhoII
XmaCI XmaI