################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI AscI AseI AsnI AspEI AviII BbrPI BcgI BclI BfrI BglII BsaAI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BsrGI BssHII Bst1107I BstBI BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco52I FbaI FseI FspI HpaI KspI MfeI MluI MroI MunI NdeI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII ScaI SfuI SgrAI SnaBI SspBI SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### Acc65I 1247 GGTACC G^GTACC AflIII 268 ACATGT A^C[GA][CT]GT Aor51HI 3747 AGCGCT AGC^GCT Asp718I 1247 GGTACC G^GTACC AvrII 3766 CCTAGG C^CTAGG BamHI 2398 GGATCC G^GATCC BbeI 2773 GGCGCC GGCGC^C BlnI 3766 CCTAGG C^CTAGG BlpI 2929 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2929 GCTGAGC GC^T[ACGT]AGC BsaBI 3989 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmI 2316 GAATGC GAATGC(1/-1) BspLU11I 268 ACATGT A^CATGT CelII 2929 GCTGAGC GC^T[ACGT]AGC DraI 6901 TTTAAA TTT^AAA Eco47III 3747 AGCGCT AGC^GCT EcoRV 1223 GATATC GAT^ATC EcoT22I 5699 ATGCAT ATGCA^T HindIII 931 AAGCTT A^AGCTT KasI 2773 GGCGCC G^GCGCC KpnI 1247 GGTACC GGTAC^C MamI 3989 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 2773 GGCGCC GG^CGCC NheI 3472 GCTAGC G^CTAGC NsiI 5699 ATGCAT ATGCA^T SalI 3516 GTCGAC G^TCGAC SexAI 2383 ACCTGGT A^CC[AT]GGT SfoI 2773 GGCGCC GGC^GCC SpeI 1311 ACTAGT A^CTAGT XbaI 3827 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcsI 948 GAATTC [GA]^AATT[CT] 2499 GAATTC ApoI 948 GAATTC [GA]^AATT[CT] 2499 GAATTC Asp700I 2862 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3163 GAAGCCATTC AspI 796 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2382 GACCTGGTC BciVI 4556 GGATAC GTATCC(6/5) 6650 GTATCC BsaWI 2378 ACCGGA [AT]^CCGG[AT] 4658 ACCGGA BsmBI 636 CGTCTC CGTCTC(1/5) 5228 CGTCTC BsrFI 4178 GCCGGC [GA]^CCGG[CT] 5070 GCCGGC BssSI 1126 CACGAG CACGAG(-5/-1) 1626 CACGAG BstEII 1215 GGTCACC G^GT[ACGT]ACC 2995 GGTCACC BstPI 1215 GGTCACC G^GT[ACGT]ACC 2995 GGTCACC Cfr10I 4178 GCCGGC [GA]^CCGG[CT] 5070 GCCGGC DrdI 3507 GACCATGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 6871 GACAGCTAGGTC EcoO65I 1215 GGTCACC G^GT[ACGT]ACC 2995 GGTCACC EcoRI 948 GAATTC G^AATTC 2499 GAATTC HincII 3360 GTCAAC GT[CT]^[GA]AC 3516 GTCGAC HindII 3360 GTCAAC GT[CT]^[GA]AC 3516 GTCGAC NaeI 4178 GCCGGC GCC^GGC 5070 GCCGGC NgoMIV 4178 GCCGGC G^CCGGC 5070 GCCGGC PflFI 796 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2382 GACCTGGTC PshAI 1428 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 6859 GACCTGTGTC SbfI 452 CCTGCAGG CCTGCA^GG 1957 CCTGCAGG SfiI 2080 GGCCCGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 4991 GGCCCTGAGGGCC SphI 1658 GCATGC GCATG^C 5204 GCATGC Sse8387I 452 CCTGCAGG CCTGCA^GG 1957 CCTGCAGG TaqI 1373 TCGA T^CGA 3517 TCGA Tth111I 796 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2382 GACCTGGTC TthHB8I 1373 TCGA T^CGA 3517 TCGA XmnI 2862 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3163 GAAGCCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 131 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AluI Alw44I AlwI AlwNI AocI ApaI ApaLI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XcmI XhoII XmaCI XmaI