##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AccIII AflIII AgeI Aor51HI
AscI AviII AvrII BcgI BglI
BlnI BsaWI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspLU11I BssHII
BssSI BstBI ClaI CpoI EagI
EclXI Eco47III Eco52I EcoRV EcoT22I
FseI FspI KspI MluI MroI
NaeI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PvuI RsrII SacII
SbfI SfiI SfuI SgrAI SpeI
Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 3677 GGTACC G^GTACC
AccII 637 CGCG CG^CG
AflII 4575 CTTAAG C^TTAAG
Alw44I 1545 GTGCAC G^TGCAC
AocI 5067 CCTCAGG CC^T[ACGT]AGG
ApaI 1096 GGGCCC GGGCC^C
ApaLI 1545 GTGCAC G^TGCAC
AseI 3129 ATTAAT AT^TAAT
AsnI 3129 ATTAAT AT^TAAT
Asp718I 3677 GGTACC G^GTACC
AspI 3537 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 9 CCCGGG C^[CT]CG[GA]G
BamHI 1711 GGATCC G^GATCC
BbrPI 1513 CACGTG CAC^GTG
BclI 5960 TGATCA T^GATCA
BfrI 4575 CTTAAG C^TTAAG
BsaBI 6000 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 9 CCCGGG C^[CT]CG[GA]G
BspHI 2821 TCATGA T^CATGA
BsrBI 5876 CCGCTC CCGCTC(-3/-3)
BsrFI 1814 ACCGGC [GA]^CCGG[CT]
BstEII 4724 GGTTACC G^GT[ACGT]ACC
BstPI 4724 GGTTACC G^GT[ACGT]ACC
BstUI 637 CGCG CG^CG
Bsu36I 5067 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1814 ACCGGC [GA]^CCGG[CT]
DraIII 2072 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3468 GACTACCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 5067 CCTCAGG CC^T[ACGT]AGG
EcoO65I 4724 GGTTACC G^GT[ACGT]ACC
EcoRI 2722 GAATTC G^AATTC
FbaI 5960 TGATCA T^GATCA
HpaI 4456 GTTAAC GTT^AAC
KpnI 3677 GGTACC GGTAC^C
MamI 6000 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 6291 CAATTG C^AATTG
MspA1I 467 CAGCTG C[AC]G^C[GT]G
MunI 6291 CAATTG C^AATTG
MvnI 637 CGCG CG^CG
NheI 2131 GCTAGC G^CTAGC
PflFI 3537 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1513 CACGTG CAC^GTG
PmlI 1513 CACGTG CAC^GTG
PshBI 3129 ATTAAT AT^TAAT
PvuII 467 CAGCTG CAG^CTG
RcaI 2821 TCATGA T^CATGA
SacI 55 GAGCTC GAGCT^C
SalI 3332 GTCGAC G^TCGAC
ScaI 2660 AGTACT AGT^ACT
SexAI 5212 ACCTGGT A^CC[AT]GGT
SmaI 9 CCCGGG CCC^GGG
SnaBI 2843 TACGTA TAC^GTA
SphI 2773 GCATGC GCATG^C
StuI 2165 AGGCCT AGG^CCT
Tth111I 3537 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5207 TCTAGA T^CTAGA
XmaCI 9 CCCGGG C^CCGGG
XmaI 9 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AclI 3760 AACGTT AA^CGTT
5684 AACGTT
AcyI 14 GGCGCC G[GA]^CG[CT]C
1304 GGCGCC
AhdI 3388 GACAGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5053 GACTATAAGTC
AspEI 3388 GACAGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5053 GACTATAAGTC
BalI 1165 TGGCCA TGG^CCA
3606 TGGCCA
BbeI 14 GGCGCC GGCGC^C
1304 GGCGCC
BbvCI 691 CCTCAGC CCTCAGC(-5/-2)
5310 GCTGAGG
BciVI 2689 GGATAC GTATCC(6/5)
5906 GTATCC
BlpI 1473 GCTCAGC GC^T[ACGT]AGC
4393 GCTTAGC
Bpu1102I 1473 GCTCAGC GC^T[ACGT]AGC
4393 GCTTAGC
BsaHI 14 GGCGCC G[GA]^CG[CT]C
1304 GGCGCC
BsmI 2586 GAATGC GAATGC(1/-1)
2832 GAATGC
Bst1107I 2978 GTATAC GTA^TAC
6322 GTATAC
BstZ17I 2978 GTATAC GTA^TAC
6322 GTATAC
CelII 1473 GCTCAGC GC^T[ACGT]AGC
4393 GCTTAGC
Eam1105I 3388 GACAGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5053 GACTATAAGTC
Hin1I 14 GGCGCC G[GA]^CG[CT]C
1304 GGCGCC
HindIII 1062 AAGCTT A^AGCTT
3842 AAGCTT
KasI 14 GGCGCC G^GCGCC
1304 GGCGCC
MluNI 1165 TGGCCA TGG^CCA
3606 TGGCCA
MscI 1165 TGGCCA TGG^CCA
3606 TGGCCA
NarI 14 GGCGCC GG^CGCC
1304 GGCGCC
NcoI 3783 CCATGG C^CATGG
5330 CCATGG
Psp1406I 3760 AACGTT AA^CGTT
5684 AACGTT
PstI 2623 CTGCAG CTGCA^G
3406 CTGCAG
SfoI 14 GGCGCC GGC^GCC
1304 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspMI BsrDI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI