##################################
# NO CUT ENZYMES (   45 enzymes) #
##################################

AflII       AgeI        Alw44I      ApaLI       AscI        
AseI        AsnI        AspI        AviII       BamHI       
BfrI        BsiWI       BspDI       Bst1107I    BstBI       
BstZ17I     ClaI        CpoI        EcoT22I     FseI        
FspI        HpaI        KspI        MfeI        MluI        
MunI        NotI        NruI        NsiI        NspV        
PacI        PflFI       PinAI       PmeI        PshBI       
PvuI        RsrII       SacII       SalI        SfuI        
SgrAI       SnaBI       SpeI        SwaI        Tth111I     


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AatII        2957  GACGTC               GACGT^C
AccIII       1899  TCCGGA               T^CCGGA
AclI         5938  AACGTT               AA^CGTT
AhdI          662  GACTGTCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      3606  AGCGCT               AGC^GCT
Asp700I      2317  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         662  GACTGTCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI          229  GGCGCC               GGCGC^C
BbsI         1102  GAAGAC               GAAGAC(2/6)
BcgI          307  GCACAGCCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         220  GGATAC               GTATCC(6/5)
BpuAI        1102  GAAGAC               GAAGAC(2/6)
BsaBI        2637  GATTTGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1899  TCCGGA               [AT]^CCGG[AT]
BseAI        1899  TCCGGA               T^CCGGA
BsmI         5765  GAATGC               GAATGC(1/-1)
Bsp1407I     6315  TGTACA               T^GTACA
BspEI        1899  TCCGGA               T^CCGGA
BspHI        5855  TCATGA               T^CATGA
BsrBI        2377  GAGCGG               CCGCTC(-3/-3)
BsrGI        6315  TGTACA               T^GTACA
BssHII       1710  GCGCGC               G^CGCGC
BssSI        1438  CTCGTG               CACGAG(-5/-1)
DrdI         2867  GACACCCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      662  GACTGTCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     3606  AGCGCT               AGC^GCT
EcoRV        2426  GATATC               GAT^ATC
HindIII      4771  AAGCTT               A^AGCTT
KasI          229  GGCGCC               G^GCGCC
MamI         2637  GATTTGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         1899  TCCGGA               T^CCGGA
NaeI         4043  GCCGGC               GCC^GGC
NarI          229  GGCGCC               GG^CGCC
NgoMIV       4043  GCCGGC               G^CCGGC
NheI         5103  GCTAGC               G^CTAGC
PaeR7I       3089  CTCGAG               C^TCGAG
Psp1406I     5938  AACGTT               AA^CGTT
RcaI         5855  TCATGA               T^CATGA
SapI         3428  GAAGAGC              GCTCTTC(1/4)
SbfI         2947  CCTGCAGG             CCTGCA^GG
ScaI         5178  AGTACT               AGT^ACT
SexAI        3462  ACCTGGT              A^CC[AT]GGT
SfoI          229  GGCGCC               GGC^GCC
SmlI         3089  CTCGAG               C^T[CT][GA]AG
SphI          911  GCATGC               GCATG^C
Sse8387I     2947  CCTGCAGG             CCTGCA^GG
SspBI        6315  TGTACA               T^GTACA
XhoI         3089  CTCGAG               C^TCGAG
XmnI         2317  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

Acc65I       3654  GGTACC               G^GTACC
             5800  GGTACC              
Asp718I      3654  GGTACC               G^GTACC
             5800  GGTACC              
AvrII        2885  CCTAGG               C^CTAGG
             4946  CCTAGG              
BclI         1034  TGATCA               T^GATCA
             3435  TGATCA              
BlnI         2885  CCTAGG               C^CTAGG
             4946  CCTAGG              
BlpI          770  GCTCAGC              GC^T[ACGT]AGC
             3359  GCTGAGC             
Bpu1102I      770  GCTCAGC              GC^T[ACGT]AGC
             3359  GCTGAGC             
BsaI         1545  GAGACC               GGTCTC(1/5)
             2875  GGTCTC              
BsiEI        2914  CGGCCG               CG[GA][CT]^CG
             3533  CGGCCG              
BspLU11I     3777  ACATGT               A^CATGT
             4564  ACATGT              
BsrFI        4043  GCCGGC               [GA]^CCGG[CT]
             5422  ACCGGC              
BstAPI        254  GCAGGCCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3069  GCAAAGTCTGC         
BstEII        122  GGTCACC              G^GT[ACGT]ACC
              439  GGTGACC             
BstPI         122  GGTCACC              G^GT[ACGT]ACC
              439  GGTGACC             
CelII         770  GCTCAGC              GC^T[ACGT]AGC
             3359  GCTGAGC             
Cfr10I       4043  GCCGGC               [GA]^CCGG[CT]
             5422  ACCGGC              
DraI         6031  TTTAAA               TTT^AAA
             6073  TTTAAA              
EagI         2914  CGGCCG               C^GGCCG
             3533  CGGCCG              
EclXI        2914  CGGCCG               C^GGCCG
             3533  CGGCCG              
Eco52I       2914  CGGCCG               C^GGCCG
             3533  CGGCCG              
EcoO65I       122  GGTCACC              G^GT[ACGT]ACC
              439  GGTGACC             
FbaI         1034  TGATCA               T^GATCA
             3435  TGATCA              
KpnI         3654  GGTACC               GGTAC^C
             5800  GGTACC              
NdeI          723  CATATG               CA^TATG
             3488  CATATG              
PshAI         116  GACCAGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             5040  GACAGCGGTC          
SfiI         1883  GGCCTGCGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             2906  GGCCAGGGCGGCC       
SspI         4572  AATATT               AAT^ATT
             6016  AATATT              


#########################################
# 3 OR MORE CUT ENZYMES (  137 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AflIII      AluI        AlwI        AlwNI       
AocI        ApaI        ApoI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbrPI       
BbvCI       BbvI        BcnI        BfaI        BglI        
BglII       BmyI        BpmI        BsaAI       BsaHI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Bsu36I      Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DraIII      DsaI        EaeI        
EarI        Eco81I      EcoNI       EcoO109I    EcoRI       
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PmaCI       PmlI        
PpuMI       PstI        PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XbaI        XcmI        XhoII       
XmaCI       XmaI