##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII AccIII AflII AhdI AscI
Asp700I AspEI BbeI BfrI BglII
BlpI Bpu1102I BseAI BsiEI BsiWI
BspEI BssHII BstAPI BstBI CelII
CpoI EagI Eam1105I EclXI Eco52I
EcoRV FseI KasI KspI MluI
MroI NaeI NarI NgoMIV NotI
NruI NspV PacI PaeR7I PmeI
PshAI PvuI RsrII SacII SalI
SbfI SfiI SfoI SfuI SnaBI
SpeI Sse8387I SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 1376 GGTACC G^GTACC
AccII 3518 CGCG CG^CG
AclI 417 AACGTT AA^CGTT
AcyI 4827 GGCGTC G[GA]^CG[CT]C
AflIII 5900 ACATGT A^C[GA][CT]GT
AgeI 3877 ACCGGT A^CCGGT
AocI 2750 CCTCAGG CC^T[ACGT]AGG
Asp718I 1376 GGTACC G^GTACC
AspI 6072 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3628 TGCGCA TGC^GCA
AvrII 127 CCTAGG C^CTAGG
BbrPI 5659 CACGTG CAC^GTG
BbvCI 803 CCTCAGC CCTCAGC(-5/-2)
BcgI 1021 GCATAGTTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4860 GTATCC GTATCC(6/5)
BclI 15 TGATCA T^GATCA
BglI 6666 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 127 CCTAGG C^CTAGG
BsaBI 2479 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 4827 GGCGTC G[GA]^CG[CT]C
BsaWI 3877 ACCGGT [AT]^CCGG[AT]
BspDI 4 ATCGAT AT^CGAT
BspLU11I 5900 ACATGT A^CATGT
BssSI 810 CACGAG CACGAG(-5/-1)
BstEII 6179 GGTTACC G^GT[ACGT]ACC
BstPI 6179 GGTTACC G^GT[ACGT]ACC
BstUI 3518 CGCG CG^CG
Bsu36I 2750 CCTCAGG CC^T[ACGT]AGG
ClaI 4 ATCGAT AT^CGAT
Eco81I 2750 CCTCAGG CC^T[ACGT]AGG
EcoO65I 6179 GGTTACC G^GT[ACGT]ACC
FbaI 15 TGATCA T^GATCA
FspI 3628 TGCGCA TGC^GCA
Hin1I 4827 GGCGTC G[GA]^CG[CT]C
HpaI 6565 GTTAAC GTT^AAC
KpnI 1376 GGTACC GGTAC^C
MamI 2479 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 3518 CGCG CG^CG
NheI 338 GCTAGC G^CTAGC
PflFI 6072 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3877 ACCGGT A^CCGGT
PmaCI 5659 CACGTG CAC^GTG
PmlI 5659 CACGTG CAC^GTG
Psp1406I 417 AACGTT AA^CGTT
ScaI 6895 AGTACT AGT^ACT
SgrAI 1410 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 7076 CCCGGG CCC^GGG
Tth111I 6072 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5369 TCTAGA T^CTAGA
XcmI 31 CCACATTGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 7076 CCCGGG C^CCGGG
XmaI 7076 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Alw44I 1925 GTGCAC G^TGCAC
3032 GTGCAC
ApaI 431 GGGCCC GGGCC^C
3758 GGGCCC
ApaLI 1925 GTGCAC G^TGCAC
3032 GTGCAC
AvaI 1898 CCCGAG C^[CT]CG[GA]G
7076 CCCGGG
BalI 4834 TGGCCA TGG^CCA
6606 TGGCCA
BamHI 367 GGATCC G^GATCC
1958 GGATCC
BsmBI 1031 CGTCTC CGTCTC(1/5)
4562 GAGACG
BsoBI 1898 CCCGAG C^[CT]CG[GA]G
7076 CCCGGG
BsrBI 3586 GAGCGG CCGCTC(-3/-3)
4484 GAGCGG
BsrFI 1411 GCCGGT [GA]^CCGG[CT]
3877 ACCGGT
Bst1107I 3452 GTATAC GTA^TAC
6113 GTATAC
BstZ17I 3452 GTATAC GTA^TAC
6113 GTATAC
Cfr10I 1411 GCCGGT [GA]^CCGG[CT]
3877 ACCGGT
DraIII 1038 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
5705 CACTTTGTG
EcoNI 1510 CCTTACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2746 CCTTCCTCAGG
HgaI 4828 GCGTC GACGC(5/10)
5359 GCGTC
HindIII 10 AAGCTT A^AGCTT
407 AAGCTT
MfeI 6118 CAATTG C^AATTG
6790 CAATTG
MluNI 4834 TGGCCA TGG^CCA
6606 TGGCCA
MscI 4834 TGGCCA TGG^CCA
6606 TGGCCA
MunI 6118 CAATTG C^AATTG
6790 CAATTG
SphI 3069 GCATGC GCATG^C
8112 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI Aor51HI ApoI AseI
AsnI AspHI AvaII BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspHI BspMI BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DrdI DsaI EaeI
EarI Eco47III EcoO109I EcoRI EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeI NdeII NlaIII
NlaIV NsiI NspI PflMI PleI
PpuMI PshBI PstI PvuII RcaI
RsaI SacI SapI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
SspBI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII