##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
Acc65I AccI AclI AflII AscI
AseI AsnI Asp718I BamHI BcgI
BclI BfrI BglII BsiWI BsmI
BspDI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI EcoRI EcoRV EcoT22I
FbaI FseI HpaI KpnI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SalI ScaI SfiI SfuI SgrAI
SnaBI SpeI SphI SspI SwaI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 4896 AAATTT [GA]^AATT[CT]
AflIII 2938 ACATGT A^C[GA][CT]GT
AgeI 512 ACCGGT A^CCGGT
AhdI 1602 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2679 GTGCAC G^TGCAC
Aor51HI 4656 AGCGCT AGC^GCT
ApaLI 2679 GTGCAC G^TGCAC
ApoI 4896 AAATTT [GA]^AATT[CT]
Asp700I 1401 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1602 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 657 CCTAGG C^CTAGG
BciVI 1169 GGATAC GTATCC(6/5)
BlnI 657 CCTAGG C^CTAGG
BspHI 896 TCATGA T^CATGA
BspLU11I 2938 ACATGT A^CATGT
BstEII 1803 GGTGACC G^GT[ACGT]ACC
BstPI 1803 GGTGACC G^GT[ACGT]ACC
Eam1105I 1602 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4656 AGCGCT AGC^GCT
EcoO65I 1803 GGTGACC G^GT[ACGT]ACC
PaeR7I 1462 CTCGAG C^TCGAG
PinAI 512 ACCGGT A^CCGGT
RcaI 896 TCATGA T^CATGA
SapI 3218 GAAGAGC GCTCTTC(1/4)
SbfI 3722 CCTGCAGG CCTGCA^GG
Sse8387I 3722 CCTGCAGG CCTGCA^GG
XbaI 2919 TCTAGA T^CTAGA
XhoI 1462 CTCGAG C^TCGAG
XmnI 1401 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 1106 TCCGGA T^CCGGA
1598 TCCGGA
AocI 1750 CCTCAGG CC^T[ACGT]AGG
2068 CCTCAGG
BbsI 936 GAAGAC GAAGAC(2/6)
1842 GTCTTC
BlpI 1218 GCTGAGC GC^T[ACGT]AGC
3055 GCTCAGC
Bpu1102I 1218 GCTGAGC GC^T[ACGT]AGC
3055 GCTCAGC
BpuAI 936 GAAGAC GAAGAC(2/6)
1842 GTCTTC
BsaBI 577 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4049 GATCAACATC
BseAI 1106 TCCGGA T^CCGGA
1598 TCCGGA
BsiEI 56 CGGCCG CG[GA][CT]^CG
3077 CGGCCG
Bsp1407I 1385 TGTACA T^GTACA
5178 TGTACA
BspEI 1106 TCCGGA T^CCGGA
1598 TCCGGA
BsrBI 76 GAGCGG CCGCTC(-3/-3)
912 GAGCGG
BsrDI 434 GCAATG GCAATG(2/0)
2431 GCAATG
BsrGI 1385 TGTACA T^GTACA
5178 TGTACA
BssSI 4359 CACGAG CACGAG(-5/-1)
4802 CTCGTG
Bsu36I 1750 CCTCAGG CC^T[ACGT]AGG
2068 CCTCAGG
CelII 1218 GCTGAGC GC^T[ACGT]AGC
3055 GCTCAGC
DraI 4889 TTTAAA TTT^AAA
4976 TTTAAA
EagI 56 CGGCCG C^GGCCG
3077 CGGCCG
EclXI 56 CGGCCG C^GGCCG
3077 CGGCCG
Eco52I 56 CGGCCG C^GGCCG
3077 CGGCCG
Eco81I 1750 CCTCAGG CC^T[ACGT]AGG
2068 CCTCAGG
HindIII 766 AAGCTT A^AGCTT
3914 AAGCTT
KspI 23 CCGCGG CCGC^GG
1808 CCGCGG
MamI 577 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4049 GATCAACATC
MroI 1106 TCCGGA T^CCGGA
1598 TCCGGA
SacI 666 GAGCTC GAGCT^C
5189 GAGCTC
SacII 23 CCGCGG CCGC^GG
1808 CCGCGG
SexAI 1681 ACCAGGT A^CC[AT]GGT
2978 ACCAGGT
SspBI 1385 TGTACA T^GTACA
5178 TGTACA
Tsp509I 1820 AATT ^AATT
4897 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AspI AvaI AvaII AviII BalI
BanI BanII BbeI BbrPI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DrdI
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflFI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmaCI XmaI