##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AclI AflIII AgeI AhdI
Aor51HI AscI AspEI AviII BsaBI
BsiEI BsiWI BspDI BspHI BspLU11I
BsrFI BssHII Bst1107I BstZ17I Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoT22I
FseI FspI MamI MfeI MluI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PinAI PmeI Psp1406I PvuI RcaI
RsrII SbfI ScaI SfiI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 3228 GGTACC G^GTACC
AflII 4544 CTTAAG C^TTAAG
Alw44I 5308 GTGCAC G^TGCAC
ApaLI 5308 GTGCAC G^TGCAC
AseI 1564 ATTAAT AT^TAAT
AsnI 1564 ATTAAT AT^TAAT
Asp718I 3228 GGTACC G^GTACC
BbrPI 3063 CACGTG CAC^GTG
BbsI 2053 GAAGAC GAAGAC(2/6)
BcgI 22 GCAGTGGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3204 GTATCC GTATCC(6/5)
BclI 720 TGATCA T^GATCA
BfrI 4544 CTTAAG C^TTAAG
BglII 1711 AGATCT A^GATCT
BpuAI 2053 GAAGAC GAAGAC(2/6)
BsaAI 3063 CACGTG [CT]AC^GT[GA]
BsmBI 3079 CGTCTC CGTCTC(1/5)
Bsp1407I 1558 TGTACA T^GTACA
BsrGI 1558 TGTACA T^GTACA
BssSI 2923 CACGAG CACGAG(-5/-1)
BstAPI 4955 GCAAGTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4018 TTCGAA TT^CGAA
BstEII 5050 GGTAACC G^GT[ACGT]ACC
BstPI 5050 GGTAACC G^GT[ACGT]ACC
DraI 4818 TTTAAA TTT^AAA
EcoO65I 5050 GGTAACC G^GT[ACGT]ACC
EcoRV 4560 GATATC GAT^ATC
FbaI 720 TGATCA T^GATCA
HpaI 5371 GTTAAC GTT^AAC
KpnI 3228 GGTACC GGTAC^C
KspI 283 CCGCGG CCGC^GG
NspV 4018 TTCGAA TT^CGAA
PmaCI 3063 CACGTG CAC^GTG
PmlI 3063 CACGTG CAC^GTG
PshBI 1564 ATTAAT AT^TAAT
SacII 283 CCGCGG CCGC^GG
SalI 563 GTCGAC G^TCGAC
SfuI 4018 TTCGAA TT^CGAA
SspBI 1558 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 563 GTCGAC GT^[AC][GT]AC
3195 GTCTAC
AccIII 4027 TCCGGA T^CCGGA
4522 TCCGGA
AcyI 107 GGCGCC G[GA]^CG[CT]C
3847 GGCGCC
Asp700I 1591 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5747 GAATAAATTC
AspI 2512 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5202 GACTGAGTC
BamHI 1412 GGATCC G^GATCC
3855 GGATCC
BbeI 107 GGCGCC GGCGC^C
3847 GGCGCC
BglI 44 GCCGGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1048 GCCAGACTGGC
BsaHI 107 GGCGCC G[GA]^CG[CT]C
3847 GGCGCC
BseAI 4027 TCCGGA T^CCGGA
4522 TCCGGA
BsmI 1640 GCATTC GAATGC(1/-1)
1920 GCATTC
BspEI 4027 TCCGGA T^CCGGA
4522 TCCGGA
BsrBI 4532 CCGCTC CCGCTC(-3/-3)
300 GAGCGG
BsrDI 4120 CATTGC GCAATG(2/0)
3673 GCAATG
BstXI 577 CCACAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2070 CCAGTGGTTTGG
EcoRI 698 GAATTC G^AATTC
2484 GAATTC
HaeII 107 GGCGCC [GA]GCGC^[CT]
3847 GGCGCC
HgaI 2948 GACGC GACGC(5/10)
1084 GCGTC
Hin1I 107 GGCGCC G[GA]^CG[CT]C
3847 GGCGCC
HindIII 4822 AAGCTT A^AGCTT
5314 AAGCTT
KasI 107 GGCGCC G^GCGCC
3847 GGCGCC
MroI 4027 TCCGGA T^CCGGA
4522 TCCGGA
NarI 107 GGCGCC GG^CGCC
3847 GGCGCC
NcoI 1036 CCATGG C^CATGG
3970 CCATGG
PflFI 2512 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5202 GACTGAGTC
PshAI 3556 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3960 GACTGCGGTC
PstI 1005 CTGCAG CTGCA^G
4620 CTGCAG
SacI 2847 GAGCTC GAGCT^C
4526 GAGCTC
SapI 148 GAAGAGC GCTCTTC(1/4)
2721 GAAGAGC
SexAI 3199 ACCAGGT A^CC[AT]GGT
5707 ACCTGGT
SfoI 107 GGCGCC GGC^GCC
3847 GGCGCC
TaqI 564 TCGA T^CGA
4019 TCGA
Tth111I 2512 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5202 GACTGAGTC
TthHB8I 564 TCGA T^CGA
4019 TCGA
XbaI 1042 TCTAGA T^CTAGA
1740 TCTAGA
XmnI 1591 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5747 GAATAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII AvrII BalI
BanI BanII BbvCI BbvI BcnI
BfaI BlnI BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XcmI XhoII XmaCI
XmaI