##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AccI AclI AgeI Alw44I ApaLI
AscI AviII BamHI BsiEI BsiWI
Bsp1407I BspDI BspLU11I BsrGI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DraIII EagI EclXI Eco52I
FseI FspI HincII HindII HpaI
MluI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI Psp1406I
PvuI RsrII SalI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 3715 GACGTC GACGT^C
AflIII 3856 ACACGT A^C[GA][CT]GT
Aor51HI 3001 AGCGCT AGC^GCT
AseI 4397 ATTAAT AT^TAAT
AsnI 4397 ATTAAT AT^TAAT
Asp700I 2878 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 3857 CACGTG CAC^GTG
BcgI 4242 GCAGGAGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5573 GTATCC GTATCC(6/5)
BclI 397 TGATCA T^GATCA
BglII 890 AGATCT A^GATCT
BsaBI 4924 GATCGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 2744 CGTCTC CGTCTC(1/5)
BspHI 4905 TCATGA T^CATGA
BssSI 2711 CTCGTG CACGAG(-5/-1)
BstEII 2165 GGTTACC G^GT[ACGT]ACC
BstPI 2165 GGTTACC G^GT[ACGT]ACC
DrdI 2386 GACACAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3001 AGCGCT AGC^GCT
EcoO65I 2165 GGTTACC G^GT[ACGT]ACC
EcoRI 4703 GAATTC G^AATTC
EcoRV 5718 GATATC GAT^ATC
EcoT22I 5802 ATGCAT ATGCA^T
FbaI 397 TGATCA T^GATCA
HindIII 5412 AAGCTT A^AGCTT
KspI 2219 CCGCGG CCGC^GG
MamI 4924 GATCGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5218 CAATTG C^AATTG
MunI 5218 CAATTG C^AATTG
NdeI 5848 CATATG CA^TATG
NsiI 5802 ATGCAT ATGCA^T
NspI 1336 GCATGT [GA]CATG^[CT]
PmaCI 3857 CACGTG CAC^GTG
PmlI 3857 CACGTG CAC^GTG
PshBI 4397 ATTAAT AT^TAAT
RcaI 4905 TCATGA T^CATGA
SacII 2219 CCGCGG CCGC^GG
SapI 1496 GCTCTTC GCTCTTC(1/4)
SbfI 832 CCTGCAGG CCTGCA^GG
SexAI 4362 ACCTGGT A^CC[AT]GGT
Sse8387I 832 CCTGCAGG CCTGCA^GG
SspI 2003 AATATT AAT^ATT
XbaI 4435 TCTAGA T^CTAGA
XmnI 2878 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 2037 TCCGGA T^CCGGA
5067 TCCGGA
AcsI 4578 GAATTT [GA]^AATT[CT]
4703 GAATTC
AhdI 1698 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3182 GACCAGCTGTC
ApoI 4578 GAATTT [GA]^AATT[CT]
4703 GAATTC
AspEI 1698 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3182 GACCAGCTGTC
AspI 1091 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4326 GACCCTGTC
AvrII 671 CCTAGG C^CTAGG
5530 CCTAGG
BbeI 4 GGCGCC GGCGC^C
3528 GGCGCC
BlnI 671 CCTAGG C^CTAGG
5530 CCTAGG
BsaAI 1751 TACGTG [CT]AC^GT[GA]
3857 CACGTG
BsaWI 2037 TCCGGA [AT]^CCGG[AT]
5067 TCCGGA
BseAI 2037 TCCGGA T^CCGGA
5067 TCCGGA
BspEI 2037 TCCGGA T^CCGGA
5067 TCCGGA
Eam1105I 1698 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3182 GACCAGCTGTC
EcoNI 355 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2581 CCTGTCAGAGG
HgaI 3814 GCGTC GACGC(5/10)
5471 GCGTC
KasI 4 GGCGCC G^GCGCC
3528 GGCGCC
MroI 2037 TCCGGA T^CCGGA
5067 TCCGGA
NarI 4 GGCGCC GG^CGCC
3528 GGCGCC
NheI 1390 GCTAGC G^CTAGC
5213 GCTAGC
PflFI 1091 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4326 GACCCTGTC
SfoI 4 GGCGCC GGC^GCC
3528 GGCGCC
SmaI 2866 CCCGGG CCC^GGG
4260 CCCGGG
Tth111I 1091 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4326 GACCCTGTC
XcmI 1842 CCACAAGGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3468 CCACAGACACCATGG
XmaCI 2866 CCCGGG C^CCGGG
4260 CCCGGG
XmaI 2866 CCCGGG C^CCGGG
4260 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AflII AluI AlwI AlwNI AocI
ApaI Asp718I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BfrI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII